p27在调节小鼠下颌牙槽骨生长发育中的作用

›› 2015, Vol. 35 ›› Issue (6): 421-424.

p27在调节小鼠下颌牙槽骨生长发育中的作用

尹颖¹,周芷萱²,陈宁²,苗登顺³

1. 南京大学医学院附属口腔医学院
2. 南京医科大学口腔医学研究所
3. 南京医科大学人体解剖学系

收稿日期:2015-03-03 修回日期:2015-03-22 出版日期:2015-06-28 发布日期:2015-07-01
通讯作者: 尹颖 E-mail:yinying5413@163.com
基金资助:
973项目

p27kip1 Regulates Alveolar Bone Formation in Postnatal Mice

Received:2015-03-03 Revised:2015-03-22 Online:2015-06-28 Published:2015-07-01

摘要/Abstract

摘要： 目的：明确细胞周期依赖性激酶抑制剂p27kip1在小鼠下颌骨发育形成过程中的作用和机制。方法：通过影像学、组织化学染色、免疫组织化学染色等方法比较分析了2周龄p27基因敲除（p27-/-）小鼠与同窝野生型（WT）小鼠下颌牙槽骨的表型差异。取下颌骨提取蛋白，利用Western blot的方法检测了Sonic Hedgehog（Shh）信号通路相关分子表达的变化。结果：与同窝2周龄WT小鼠相比，p27-/-小鼠下颌骨体积增大，矿化显著增强；下颌牙槽骨碱性磷酸酶（ALP）阳性面积和I型胶原（Col-I）阳性面积的增加，表明p27-/-小鼠成骨细胞活性和功能显著增加。同时发现Shh信号通路相关蛋白Shh、Patched、Smoothened、Gli2的表达水平在p27-/-小鼠均显著上调。结论：我们的结果说明细胞周期依赖性激酶抑制剂p27kip1能够通过抑制Shh信号通路，抑制细胞增殖，发挥负性调控小鼠颌骨生长发育的作用。本研究不仅揭示了p27kip1在调控小鼠颌骨生长发育中的作用机制，而且为p27kip1作为调节颌骨生长发育的新靶标提供了理论和实验依据。

关键词: p27, 牙槽骨, 发育

Abstract: Objective: To explore the mechanism of action of the p27kip1 in intramembranous ossification. Methods: The phenotypes of the mandible were compared between p27kip1 gene knockout (p27-/-) mice and their wild-type littermates at 2 weeks of age by radiograph, histochemistry and immunohistochemistry. Sonic Hedgehog (Shh) pathway is analyzed by Western blot. Results: Compared with wild-type mice, the volume and mineral density was increased in the mandibles. Both the alkaline phosphatase positive areas and type I collagen immunopositive areas were increased significantly in the mandibles, whereas protein expression levels of Shh, Patched, Smoothened and Gli2 were up-regulated in mandibles from p27-/- mice compared with those from wild-type mice. Conclusion: Results from this study indicate that p27 plays a negative regulatory role in alveolar bone development by inhibiting shh signal pathway and cell proliferation. This study elucidates the role and mechanism of p27 in regulating tooth and mandible development, thus provide experimental and theoretical basis for p27 as a new target for the growth and development of tooth and mandible.

Key words: p27, Alveolar bone, development

尹颖周芷萱陈宁苗登顺. p27在调节小鼠下颌牙槽骨生长发育中的作用[J]. 口腔医学, 2015, 35(6): 421-424.

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