›› 2015, Vol. 35 ›› Issue (6): 429-432.

• 基础研究 • 上一篇    下一篇

舌癌酸性环境下雷公藤多甙对单核/巨噬细胞杀伤活性的影响

任柏林1,冯红超2,宋宇峰3,马洪3   

  1. 1. 广州市荔湾区口腔医院
    2. 贵阳市口腔医院
    3. 贵阳医学院
  • 收稿日期:2015-01-08 修回日期:2015-03-11 出版日期:2015-06-28 发布日期:2015-07-01
  • 通讯作者: 冯红超 E-mail:hongchaofeng126@126.com
  • 基金资助:
    贵州省省科技厅科技计划研究项目

Effects of tripterygium wilfordii polyglycosidium on the cytotoxicity function of mononuclear-macrophages in acid environment of carcinoma of tongue

  • Received:2015-01-08 Revised:2015-03-11 Online:2015-06-28 Published:2015-07-01

摘要: 目的 通过模拟舌癌局部的酸性环境,加入雷公藤多甙(TWP),检测其对单核/巨噬细胞杀伤肿瘤细胞功能的影响。方法在pH值6.6,6.8酸性环境中,人单核/巨噬细胞THP-1与人舌鳞癌细胞Tca8113共同培养,经不同浓度的TWP干预,MTT法检测Tca8113细胞数量变化,观察此舌癌酸性环境下TWP对单核/巨噬细胞杀伤舌癌细胞作用的影响。结果酸性环境下,经不同浓度TWP干预,THP-1细胞与Tca8113细胞共同培养4小时,THP-1细胞杀伤活性随TWP浓度增加而增大,与浓度成正相关(P<0.05);pH值为6.6,TWP浓度为5×10-1μg/ml时,THP-1细胞杀伤活性随时间延长而增加,与时间成正相关(P<0.05)。结论体外模拟的舌癌酸性环境中,经一定浓度的雷公藤多甙干预,可使单核/巨噬细胞杀伤活性较未干预组增强,与药物浓度及时间成正相关。

关键词: 酸性环境, 单核/巨噬细胞, 雷公藤多甙, 舌鳞状细胞癌

Abstract: ObjectiveTo investigate the effects of tripterygium wilfordii polyglycosidium (TWP) on the cytotoxicity function of mononuclear-macrophagesin vitro acid environment of squamous-cell carcinoma of tongue.Methods MTT assay was used totest the effects on the cytotoxicity of THP-1 monocytes which were interfered by different concentrations of TWP, and co-cultured with Tca8113 cells in vitro acid environments (pH6.6 or 6.8). Results MTT assay showed thatinterfered by different concentrations of TWP and co-cultured with Tca8113 cells for 4h in the identical vitro acidenvironment(pH6.6 or 6.8), the cytotoxicity of THP-1 monocytes increased as the concentration of TWP increased, and was significantly positively related to the concentration of TWP (P<0.05). The cytotoxicity also increased with time (p<0.05) at pH6.6 and TWP of 5×10-1μg/ml, which was significantly positively related to time (P<0.05). Conclusion Cultured in vitro acid environment of squamous-cell carcinoma of tongue, The cytotoxicity function of mononuclear-macrophages interfered by TWP of certain concentrationincreased compared to those with no interference, and presented significantly positive correlations withTWP concentrations and time.

Key words: acid environment, mononuclear-macrophage, tripterygium wilfordii polyglycosidium(TWP), squamous-cell carcinoma of tongue

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