氯通道阻断剂NPPB对鸡喙外胚间充质细胞增殖分化影响的研究

›› 2015, Vol. 35 ›› Issue (6): 438-441.

氯通道阻断剂NPPB对鸡喙外胚间充质细胞增殖分化影响的研究

田美玉¹,张静²,陈晓红，孟繁荣¹,杨婷³

1. 中国医科大学航空总医院
2. 徐州市中心医院
3. 第四军医大学口腔医学院

收稿日期:2013-10-31 修回日期:2014-02-07 出版日期:2015-06-28 发布日期:2015-07-01
通讯作者: 田美玉 E-mail:mytian32502@163.com

Study on effect of Chloride channel blocker NPPB on the proliferation and differentiation of chicken mandibular mesenchymal cells

Received:2013-10-31 Revised:2014-02-07 Online:2015-06-28 Published:2015-07-01

摘要/Abstract

摘要： 目的氯离子通道阻断剂NPPB对鸡喙外胚间充质细胞(chicken mandibular mesenchymal cells，CMMC)增殖及分化能力的影响。方法应用MTT、实时定量PCR的方法，观察NPPB阻断氯离子通道后，鸡喙外胚间充质细胞在AA-BGP诱导成骨过程中，细胞增殖及Sox9mRNA、Runx2mRNA及Col10mRNA的表达的变化。结果在AA-BGP诱导组及无AA-BGP诱导组，NPPB均抑制了CMMC的增殖(P<0.05)。在AA-BGP诱导组及无AA-BGP诱导组，NPPB下调Col10mRNA及Runx2mRNA的表达水平均有显著性差异(P<0.05),Sox9mRNA的表达水平没有显著改变。结论阻断氯离子通道可抑制鸡喙外胚间充质细胞增殖,并影响细胞向肥大化的软骨细胞分化，可能通过调节Runx2水平影响分化。

关键词: 氯离子通道, NPPB, 鸡喙下颌外胚间充质细胞, 细胞增殖分化

Abstract: Objective To study the effect of Chloride channel blocker NPPB on the proliferation and differentiation of chicken mandibular mesenchymal cells (CMMC). Methods In the study, chicken mandibular mesenchymal cells (CMMC) were isolated and chondrocyte maturation was induced by ascorbic acid and b-glycerophosphate (AA-BGP). The effect of the chloride channel inhibitor NPPB [5-nitro-2-(3-phenylpropylamino)benzoic acid] on regulation of growth, and changes in Col10、Sox9 and Runx2mRNA genes expressions were observed with MTT and real-time PCR assays. Results NPPB inhibited the growth of the CMMC with/without AA-BGP treatment(P< 0.05). NPPB antagonized expression of typeX collagen and Runx2 (P < 0.05) with/ without AA-BGP treatment, whilst it had no effect on the early chondrocyte differentiation marker Sox9. Conclusion We conclude that chloride channel in CMMC plays an important role in regulating chondrocyte proliferation and plays a major role in the progression of chondrocyte differentiation into hypertrophic cartilage. The data from the current study suggest that maybe chloride channel works in chondrocyte differentiation by regulating the Runx2 level.

Key words: chloride channel, NPPB, chicken mandibular mesenchymal cells, cell proliferation and differentiation

中图分类号:

R349.5

田美玉张静陈晓红，孟繁荣杨婷. 氯通道阻断剂NPPB对鸡喙外胚间充质细胞增殖分化影响的研究[J]. 口腔医学, 2015, 35(6): 438-441.

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