›› 2021, Vol. 41 ›› Issue (7): 588-593.

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Regulatory effects of hyperbaric oxygen on cell apoptosis and apoptosis factors expression in gingival cells of diabetes-associated periodontitis

  

  • Received:2020-09-15 Revised:2020-11-16 Online:2021-07-28 Published:2021-07-28
  • Contact: Tie-Lou CHEN E-mail:xbctl@sh163.net

Abstract: Objective To investigate the regulatory effects of hyperbaric oxygen (HBO) on cell apoptosis and apoptosis factors expression of diabetesassociated periodontitis (DAP). Methods Sixty cases of DAP were divided into 3 groups, 20 cases in each group. The first group was treated with 0.25 MPa hyperbaric oxygen (HBO) for 2 weeks; the second group was treated with periodontal scaling and root planning (SRP); the third group was treated with HBO combining SRP. Periodontal clinical indicators(PLI, SBI, GI, PD, AL) in each group before and one month after treatment were measured. The apoptosis of gingival cells was observed by HE staining, TUNNEL staining and transmission electron microscopy. The IL1β, TNFα, PGE2 and Caspase3 expression in gingival tissues were detected by immunohistochemistry (IHC). Results The periodontal indices of SBI, GI, PD significantly decreased in HBO group, SRP group and HBO+SRP group at one month after treatment than pretreatment. Cell apoptosis and percentage of cell apoptosis in gingival epithelium and lamina propria decreased significantly. The expressions of IL1β, TNFα, PGE2 and Caspase3 in gingival at one month after treatment significantly decreased than pretreatment. There were significantly differences between the clinical indices (SBI, GI, PD, AL) and the percentage of apoptotic cells. The expressions of IL1β, TNFα, PGE2 and Caspase3 in gingival of HBO+SRP group were significantly lower than those of HBO group and SRP group. The positive expressions of IL1β, TNFα, PGE2 were macrophages and lymphocytes, and the positive expressions of Caspase3 were epithelial basal cells and spinous cells. Conclusion HBO could reduce the expression of IL1β, TNFα, PGE2 and Caspase3 in gingival to reduce cell apoptosis. HBO combined with SRP displays better efficacy which could maintain for more than one month.

Key words: hyperbaric oxygen, diabetes-associated periodontitis, cell apoptosis, inflammatory mediators, apoptosis factors.

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