载锌纳米线修饰钛表面的血管内皮细胞生物学行为研究

doi:10.13591/j.cnki.kqyx.2022.05.004

Abstract

Abstract: Objective To investigate the effects of titanium surface modified with zinc-containing nanowire on the behavior of vascular endothelial cells. Methods After polishing up, acid-etching and alkali-heat treatment, the titanium surface modified with nanowires (NW-Ti) and zinc-containing nanowires (Zn-NW-Ti) were prepared on the commercially pure titanium(cp-Ti) surfaces. The cp-Ti were set as the control group, and two modified titanium surfaces were set as the experimental groups. Scanning electron microscopy(SEM), X-ray photoelectron spectroscopy (XPS) and contact angle meter were used to analyze the surface morphology, elemental compositions and hydrophi licity of three specimens. The concentration of zinc ions released from Zn-NW-Ti was tested by zinc ion releasing kit. Human umbilical vein endothelial cells (HUVECs) were seeded on different titanium surfaces to study the effects on angiogenesis properties by cell adhesion, proliferation, migration and real-time quantitative PCR assays. Results Trace zinc element was detected on Zn-NW-Ti, which presented a uniform topological structure of nanowires. The concentration of zinc ions released from Zn-NW-Ti was approximately 1 mg/L. Compared with the other two groups, Zn-NW-Ti could significantly promote the adhesion, proliferation, migration of HUVECs, and up-regulate mRNA levels of angiogenic factors: HIF-1α and VEGF-A. Conclusion The titanium surface modified with zinc-containing nanowires can promote the adhesion, proliferation, migration and angiogenic gene expression of vascular endothelial cells.

Key words: zinc, nanowires, titanium, endothelial cell

CLC Number:

R783.1

SU Shan, ZHU Wenqing, QIU Jing. The in vitro study on biological behaviors of endothelial cells on the titanium surface modified with zinc-containing nanowires[J]. Stomatology, 2022, 42(5): 404-410.

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The in vitro study on biological behaviors of endothelial cells on the titanium surface modified with zinc-containing nanowires

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