浮游状态和生物膜状态白念珠菌总RNA提取方法的比较研究

doi:10.13591/j.cnki.kqyx.2022.05.006

Abstract

Abstract: Objective To provide an effective method to obtain total RNA of Candida albicans(C.albicans) in biofilm and planktonic states by comparing different extraction approaches. Methods Biofilms and planktonic C.albicans SC5314 were incubated for 24 h. Total RNA of C. albicans were extracted using modified Trizol method and modified hot phenol method, respectively. The difference in output, purity and integrity of total RNA extracted by two methods were compared by ultraviolet spectrophotometer, and further confirmed using agarose gel electrophoresis and RT-PCR. Results Modified Trizol method appeared more efficient than modified hot phenol method with less operation time (P＜0.000 5). The purity of the total RNA extracted by modified Trizol method was higher than that by modified hot phenol method (P<0.05). The result of electrophoresis showed that there were three clear bands, 28S rRNA, 18S rRNA and 5S rRNA in total RNA extracted by both modified Trizol method and modified hot phenol method. The result of RT-PCR confirmed the purity and integrity of total RNA of C.albicans with clear target bands. Conclusion Modified Trizol method contributes to more stable purity of the total RNA of C.albicans extracted in the form of planktonic than modified hot phenol method. Moreover, the modified Trizol method is rapid with easy operation and can be applied in experiments with efficiency.

Key words: Candida albicans, RNA extraction, Trizol, biofilm, planktonic

CLC Number:

R379.4

DUAN Wei, LIU Siqi, HUANG Yun, GU Ning, WEI Xin. A comparative study on the extraction methods of Candida albicans in the form of planktonic and biofilm[J]. Stomatology, 2022, 42(5): 416-419.

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A comparative study on the extraction methods of Candida albicans in the form of planktonic and biofilm

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