甘草多糖抑制唾液腺腺样囊性癌生长和迁移能力的研究

doi:10.13591/j.cnki.kqyx.2025.11.005

Abstract

Abstract:

Objective To explore the effects of glycyrrhizic polysaccharide(GP) on the biological activity of salivary adenoid cystic carcinoma tumor cells and its mechanisms of anti-tumor action. Methods GP was prepared, and human adenoid cystic carcinoma high-metastatic cell line SACC-LM and low-metastatic cell line SACC-83 were used as target cells. Different concentrations of GP were used for intervention. The cell counting kit-8 (CCK-8) assay was employed to detect cell proliferation and apoptosis. Reverse transcription polymerase chain reaction (RT-PCR) and Western blot experiments were conducted to detect the expression of genes and proteins related to tumor invasion and metastasis. The scratch assay was used to observe the effect of GP on the migration ability of SACC cells. In vivo experiments were conducted to verify the inhibitory effect of GP on tumor cells. Results Glycyrrhiza polysaccharides significantly inhibited the proliferation and migration of the highly metastatic human adenoid cystic carcinoma cell line SACC-LM by regulating the expression of epithelial-mesenchymal transition (EMT)-related markers. In vivo experiments showed that glycyrrhiza polysaccharides had no significant hepatotoxicity, could significantly reduce the volume and weight of tumors, and inhibit the growth rate of tumors in nude mice. Conclusion GP has a certain inhibitory effect on the growth and migration ability of SACC.

Key words: salivary adenoid cystic carcinoma, glycyrrhizic polysaccharide, migration

CLC Number:

R780.2

LIU Tangjun, SHAN Tianyu, WANG Xinwei, SUN Dandan, LIU Jiajia, LIU Yunxia. Research on the inhibition of glycyrrhizic polysaccharide on the growth and migration of salivary adenoid cystic carcinoma cells[J]. Stomatology, 2025, 45(11): 826-831.

Figures/Tables 6

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References 23

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基因名称	上游引物(5'-3')	下游引物(5'-3')
BTBD7	AGTCAAATGCCTGGTTACGG	TGTCTGGCACATTGGACATT
FN	GAGAATAAGCTGTACCATCGCAA	CGACCACATAGGAAGTCCCAG
E-cadherin	ATTTTTCCCTCGACACCCGAT	TCCCAGGCGTAGACCAAGA
Vimentin	GGCGAGGAGAGCAGGATTTC	TGGGTATCAACCAGAGGGAGT
β-actin	CTGCAATCCGAAAGAAGCTG	ATCTTCAAACCTCCATGATG

Research on the inhibition of glycyrrhizic polysaccharide on the growth and migration of salivary adenoid cystic carcinoma cells

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