血小板衍生生长因子受体α基因在

›› 2015, Vol. 35 ›› Issue (12): 1008-1012.

血小板衍生生长因子受体α基因在

仵素珍,康鹏,肖文林,王双义,许尧祥

青岛大学附属医院黄岛院区

收稿日期:2015-06-23 修回日期:2015-08-17 出版日期:2015-12-28 发布日期:2015-12-15
通讯作者: 肖文林 E-mail:wenlinxiao@sina.com
基金资助:
靶向求源探索 Dhcr7 基因沉默影响腭胚突融合的机制及防治的基础研究;靶向下调胆固醇代谢关键基因Dhcr7表达诱发腭裂形成的机制

The study of PDGFRα gene expression in the C57BL/6J mouse embryo palate shelves

Received:2015-06-23 Revised:2015-08-17 Online:2015-12-28 Published:2015-12-15

摘要/Abstract

摘要： 目的观察小鼠胚胎腭突融合过程中血小板衍生生长因子受体α基因（platelet derived growth factor receptor alpha,PDGFR-α）的表达特点。方法 C57BL/6J近交系受孕小鼠分别于妊娠13.5 d（Gestation Day,GD13.5）、GD14.5、GD15.0、及GD15.5时断颈处死。取小鼠胚头及腭突，免疫组织化学检测不同胚胎发育时期的腭突组织中PDGFR-α蛋白的表达定位；Western blot法检测PDGFR-α蛋白的表达变化。结果免疫组化检测结果显示PDGFR-α在小鼠腭突中特异性表达：在GD13.5时主要表达在腭突上皮，而在GD14.5、GD15.0、GD15.5时在间充质和上皮都有表达，且在GD14.5时PDGFR-α的表达水平达到高峰。Western blot检测PDGFR-α在腭突不同发育时期的蛋白表达变化和免疫组化检测结果趋势一致。在GD14.5时，PDGFR-α的表达水平达到高峰，和GD13.5、GD15.0及GD15.5蛋白表达水平相比差异有统计学意义。结论研究证实PDGFR-α在小鼠胚胎腭突发育过程中存在时空表达变化的特征。PDGFR-α可能与胚胎腭突的发生有关。

关键词: C57BL/6J小鼠, 胚胎, 腭突, 血小板衍生生长因子受体α

Abstract: Objective To observe the expression of PDGFRα in the development stages of mouse embryo palatal shelves.Methods Palatal shelves of C57BL/6J inbred mouse embryos were obtained on the day of GD13.5,GD14.5 and GD15.5 respectively by using stereoscope microscope.The expression location of PDGFRα protein in the palatal shelf was observed by immunohistochemistry（IHC） staining; and the quantities expression of PDGFRα protein were examined by Western blot（WB） analysis.Results IHC staining showed that PDGFRα was specifically expressed in mouse palatal shelves.In GD13.5,PDGFRα expressed mainly in the palatal epithelium; but in GD14.5,GD15.0,and GD15.5,the PDGFRα expressed both in mesenchymal cells and epithelial cells.The WB analysis showed that proteins expression of PDGFRα in different developmental stages of palatal was consistent with IHC results.In GD14.5，the expression level of PDGFRα reached the peak.The proteins expression in GD13.5,GD15.0 or GD15.5 was significantly different compared with that of in GD14.5.Conclusions The study showed PDGFRα had spatiotemporal expression features during the development stages of mouse embryo palatal shelves.PDGFRα gene may be associated with the development of palatal shelves.

Key words: C57BL/6J mouse, embryo, palatal shelves, PDGFRα

中图分类号:

R394.3

仵素珍康鹏肖文林王双义许尧祥. 血小板衍生生长因子受体α基因在[J]. , 2015, 35(12): 1008-1012.

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