感染根管中牙龈卟啉单胞菌fimA基因多态性研究

摘要/Abstract

摘要：

目的　研究根尖周炎患牙感染根管内牙龈卟啉单胞菌（P. gingivalis）不同fimA基因型的分布情况。方法　收集200例感染根管内样本，经DNA抽提后使用16S rDNA PCR方法检测P. gingivalis。在P. gingivalis检出阳性样本中，根据各fimA基因型的特异引物，采用PCR检测不同fimA基因型菌株的分布，并通过Pearson χ2检验对不同fimA基因型与临床症状进行相关性分析。结果　感染根管中P. gingivalis的检出率为48%，其中79例样本只检测到1种fimA基因型P. gingivalis菌株（82.3%）；12例样本检测到2种或2种以上fimA基因型P. gingivalis （12.5%）。各fimA基因型P. gingivalis的检出情况：Ⅰ型29.2%、Ⅰb型8.3%、Ⅱ型37.5%、Ⅲ型14.6%、Ⅳ型19.8%。各型fimA基因与临床症状之间无统计学相关性（P＜0.05）。结论　感染根管内P. gingivalis的fimA基因存在基因多态性，其中以fimAⅡ型P. gingivalis菌株检出率最高，其次为Ⅰ型和Ⅳ型。

关键词: 根尖周感染, 感染根管, 牙龈卟啉单胞菌, fimA基因, 基因多态性

Abstract: Objective: To investigate the distribution of different fimA genotypes of Porphyromonas gingivalis (P. gingivalis) strains in root canals among periapical periodontitis patients. Method: A total of 200 samples of infected root canals with periapical periodontitis were collected. After DNA was extracted from the samples, 16S rRNA gene-based polymerase chain reaction (PCR) method was applied to identify the appearance of P. gingivalis. Among the P. gingivalis-positive samples, fimA type-specific primers were used to amplify the target DNA fragments by PCR, aiming to obtain the information about the distribution of different fimA genotypes. Pearson χ2 test was performed to investigate the correlation between fimA types and symptoms. Result: The presence of P. gingivalis was demonstrated in 48% of total number of infected root canals. A single fimA genotype was detected in 79 samples (82.3%). Combinations of up 2 different genotypes were detected in 12 cases. The distribution of six fimA genotypes among the P. gingivalis-positive samples was as follows: type I, 29.2%; type Ib, 8.3%, type II, 37.5%; type III, 14.6%; type IV, 19.8% and type V, 0.00%. There is no significant correlation between fimA genotypes and symptoms of periapical infection. Conclusion: There is fimA genetic polymorphism of P. gingivalis in infected root canals from different patients with periapical periodontitis. Relevance ratio of type II fimA is the highest, followed by type I and IV.

Key words: Apical periodontitis, infected root canals, Porphyromonas gingivalis, fimA genotype, gene polymorphism

中图分类号:

R780.2

王畅唐子圣漆正楠毛骁俊何剑亮诸晓丹邹岩黄正蔚何智妍朱彩莲. 感染根管中牙龈卟啉单胞菌fimA基因多态性研究[J]. , 2016, 36(1): 18-21.

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