流体压力对骨髓间充质干细胞软骨向分化影响的体外实验研究

›› 2016, Vol. 36 ›› Issue (6): 481-484.

• 基础研究 • 下一篇

流体压力对骨髓间充质干细胞软骨向分化影响的体外实验研究

易飞舟¹,赵萤²,张旻²

1. 安徽合肥解放军第105医院
2. 第四军医大学口腔医院

收稿日期:2015-12-11 修回日期:2016-02-26 出版日期:2016-06-28 发布日期:2016-06-30
通讯作者: 张旻 E-mail:zhangmin@fmmu.edu.cn
基金资助:
MGF在力学刺激促PDLSCs/PRF修复脱位再植牙牙周损伤中的作用及机理研究;一种新的力学敏感分子ANTXR1在压力促BMSCs成软骨响应中的作用及力学信号传导机制

Effects of hydrolic pressure on chondrogenic differentiation of bone marrow mesenchymal stem cells

Received:2015-12-11 Revised:2016-02-26 Online:2016-06-28 Published:2016-06-30

摘要/Abstract

摘要： ［摘要］目的探讨持续性与周期性流体静压力对骨髓间充质干细胞（BMSCs）软骨向分化潜能的影响。方法采用全骨髓贴壁法分离培养大鼠BMSCs，并采用成骨成脂分化鉴定。细胞随机分为5组，分别为对照组，持续性静压力45 kPa、90 kPa组，周期性动压力0~45 kPa、0~90 kPa作用组，压力刺激组细胞采用每天1 h连续2 d的加载方式。采用Real-time PCR对细胞成软骨分化标志基因Sox9、Aggrecan及Col-Ⅱ表达水平进行检测。结果 45 kPa、90 kPa的静压力及0~45 kPa、0~90kPa动压力作用下，BMSCs中Sox9的基因表达量显著高于对照组（P<0.05）；45 kPa，90 kPa，0~90 kPa组中Aggrecan mRNA的表达量明显升高（P<0.01）；90 kPa，0~90 kPa压力作用组中Col-Ⅱ的表达量显著高于对照（P<0.01）。结论压力微环境刺激可明显促进BMSCs成软骨标志基因的表达，且持续90 kPa的压力作用下BMSCs成软骨分化效果显著。

关键词: 流体压力, 骨髓间充质干细胞, 成软骨分化

Abstract: Objective　To investigate the different effects of continuous and cyclic hydrolic pressure on marker gene expression of chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Methods: BMSCs were obtained by whole bone marrow adherent separation culture method and identified by osteogenic and adipogenic induction assays. BMSCs were treated by continuous hydrostatic pressure at 45, 90kPa and cyclic hydrostatic pressure at 0~45, 0~90kPa. Four groups were treated with pressure 1 h per day for 2 days. The expression of Sox9, Aggrecan and Col-Ⅱ was detected by Real-time PCR. Results: Real-time PCR showed that under pressure the expression Sox9 was enhanced. The expression of Aggrecan in the cells under 45, 90 and 0~90kPa was significant higher than that of control group. The expression of Col-Ⅱgene was significantly increased under 90 and 0~90 kPa .Conclusions: Hydrolic pressure could promote the chondrogenic differentiation of BMSCs. The effect of 90 kPa on the chondrogenic differentiation is remarkable.

Key words: Hydrolic pressure, Bone marrow mesenchymal stem cells (BMSCs), chondrogenic differentiation

中图分类号:

R393

易飞舟赵萤张旻. 流体压力对骨髓间充质干细胞软骨向分化影响的体外实验研究[J]. , 2016, 36(6): 481-484.

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流体压力对骨髓间充质干细胞软骨向分化影响的体外实验研究

Effects of hydrolic pressure on chondrogenic differentiation of bone marrow mesenchymal stem cells

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