BioAggregate和MTA对体内破骨细胞分化和功能的影响

摘要/Abstract

摘要： ［摘要］目的研究口腔生物陶瓷材料BioAggregate及MTA对体内LPS诱导的炎性骨吸收的影响。方法 6周龄C57/BL6雄性小鼠随机分为四组：PBS组、LPS组、BioAggregate+LPS组和MTA+LPS组，每组6只。LPS干预小鼠7 d后，取出颅盖骨进行显微-CT扫描、HE染色、酶组织化学染色和组织免疫荧光染色，观察骨破坏面积、破骨细胞的形成以及组织蛋白酶K（cathepsin K）的表达。结果 BioAggregate和MTA浸提液明显减少LPS诱导的破骨细胞形成和小鼠颅盖骨炎性骨破坏。与破骨细胞功能密切相关的组织蛋白酶K的表达在BioAggregate和MTA浸提液的作用下被显著下调。结论新型口腔生物陶瓷材料BioAggregate和传统材料MTA能够抑制体内炎性骨吸收。

关键词: BioAggregate, 三氧化矿物凝聚体（MTA）, LPS, 破骨细胞, 骨吸收, 骨吸收

Abstract: Objective　 To investigate the effect of BioAggregate on LPS-induced bone destruction in vivo, and to compare its performance with that of mineral trioxide aggregate. Methods Six-week-old C57/BL6 male mice were divided into the injection group containing 10 mg/kg LPS with or without the material extracts (n=6) and the vehicle-injection group with the culture medium alone (n=6). After LPS stimulation for 7 days, mice were sacrificed and calvarial bones were prepared for micro-computed tomography scanning, histologic analysis and double immunofluorescence staining. Results BioAggregate and MTA extracts markedly reduced osteoclast numbers and attenuated bone resorption in LPS-challenged mouse calvaria. The expression level of osteoclastogenic cathepsin K was also decreased by BioAggregate and MTA extracts. Conclusions BioAggregate and MTA showed comparable inhibitory effect on osteoclast differentiation and inflammatory bone resorption in vivo.

Key words: BioAggregate, MTA, osteoclast

中图分类号:

R781.3

王维倩刘秀艳李雪李靖敏. BioAggregate和MTA对体内破骨细胞分化和功能的影响[J]. , 2016, 36(6): 503-507.

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