2种方法使用生物活性玻璃脱敏剂对牙本质小管封闭作用的场发射扫描电镜观察

›› 2016, Vol. 36 ›› Issue (11): 997-1001.

2种方法使用生物活性玻璃脱敏剂对牙本质小管封闭作用的场发射扫描电镜观察

马骞¹,罗瑞²,王婕³,王天达¹,陈亚明¹

1. 南京医科大学附属口腔医院
2. 南京医科大学附属口腔医院综合科
3. 江苏省口腔医院

收稿日期:2016-06-01 修回日期:2016-07-22 出版日期:2016-11-28 发布日期:2016-11-29
通讯作者: 陈亚明 E-mail:593983325@qq.com
基金资助:
江苏高校优势学科建设工程资助项目;南京市科技计划项目;南京医科大学口腔医学院横向合作项目

Scanning electron microscope observation of occlusion effect on dentinal tubule by two application methods of bioglass

Received:2016-06-01 Revised:2016-07-22 Online:2016-11-28 Published:2016-11-29

摘要/Abstract

摘要： 目的使用场发射扫描电镜观察涂抹法和透明压膜托盘法使用生物活性玻璃脱敏剂在短时间内对牙本质小管的封闭效果，为增强生物活性玻璃的脱敏疗效提供新思路。方法制取56颗离体牙的牙本质片，6%柠檬酸脱矿后按处理方法和处理时间不同随机分为7组，涂抹法1 d组（A1组），涂抹法3 d组（A2组），涂抹法7 d组（A3组），压膜法1 d组（B1组），压膜法3 d组（B2组）,压膜法7 d组（B3组），空白对照组。通过FESEM观察比较各组牙本质小管封闭效果。结果涂抹法和透明压膜托盘法使用生物活性玻璃脱敏剂后均能很好的封闭牙本质小管，牙本质小管内均可见明显矿化物沉积，A1、A2、A3、B1、B2、B3组牙本质小管的暴露率分别为（6.29±1.18）%、（3.40±0.84）%、（1.23±0.43）%、（5.31±0.97)%、（1.40±0.30）%、（0.36±0.15）%，A2组>B2组，A3组>B3组，差异有统计学意义；空白对照组牙本质小管口开放，周界清晰。结论透明压膜托盘法在短时间内可以增强生物活性玻璃对敏感牙本质小管的封闭效果，与涂抹法相比，封闭效果有提高，差异有统计学意义。

关键词: 生物活性玻璃, 再矿化, 透明压膜托盘法, 场发射扫描电镜

Abstract: Objective The study was carried out to evaluate In Vitro occlusion effect on dentinal tubule orifice by smearing or transparent pressureswage application of bioactive glass desensitizer using FESEM. To provide rationale and evidence for strengthening efficacy of bioactive glass desensitizing treatment. Methods 56 dentin slices were prepared from extracted teeth and treated with 6% citric acid solution to demineralization, then dentin slices were randomly divided into 7 groups according to different treating methods and durations: smearing 1day group (A1), smearing 3 day group (A2), smearing 7 day group (A3), pressureswage 1day group (B1), pressureswage 3day group (B2), pressureswage 7day group (B3) and negative control group. Occlusion effect was observed in dentinal tubule orifice in each group via Field emission scanning electron microscope (FESEM). Result FESEM results indicated that dentinal tubule was well occluded after smearing or transparent pressureswage application of bioactive glass desensitizer. Apparent mineral deposition was observed in dentinal tubule. Dentinal tubule exposure rates were A1(6.29±1.18)%、A2(3.40±0.84)%、A3(1.23±0.43)% 1、3、7 days after smearing method. Dentinal tubule exposure rates were B1(5.31±0.97)%、B2(1.4±0.30)%、B3(0.36±0.15)% 1、3、7 days after pressureswage method. The difference of dentinal tubule exposure between A1and B1 was not statistically significant (p value=0.42). While differences of exposure in A2/B2 and A3/B3 were statistically significant (p value=0.007 and 0.013). In negative control group, dentinal tubule was open with clear border and dentinal tubule exposure rate was (19.11±1.27)%. Conclusion transparent pressureswage method can improve occlusion effect of bioactive glass desensitizer in a short period of time. Compared with smearing method the improvement is significant.

Key words: bioactive glass, remineralization, transparent pressureswage method, field emission scanning electron microscope

中图分类号:

R781.05

马骞罗瑞王婕王天达陈亚明. 2种方法使用生物活性玻璃脱敏剂对牙本质小管封闭作用的场发射扫描电镜观察[J]. , 2016, 36(11): 997-1001.

参考文献

[1]Rees JS,Addy M.A cross-sectional study of dentin hypersensitivity[J].J Clin Periodontol,2002,29:997-1003
[2]Litkowski LJ.[J].Hack GD,Greenspan DC. Compositions containing bioactive glass and their use in treating tooth hypersensitivity. US Patent 5,735,942. April,1998,7:-
[3]Litkowski LJ.[J].Hack GD,Greenspan DC. Methods of treatment using bioactive glass. US Patent 6,086,374. July,2000,11:-
[4]Du MQ,Tai BJ,Jiang H,et al.Clinical evaluation of a dentifrice containing calcium sodium phosphosilicate (novamin) for the treatment of dentin hypersensitivity[J].Am J Dent,2008,21(4):210-214
[5]Pradeep AR,Sharma A.Comparison of clinical efficacy of a dentifrice containing calcium sodium phosphosilicate to a dentifrice containing potassium nitrate and to a placebo on dentinal hypersensitivity: a randomized clinical trial[J].J Periodontol,2010,81(8):1167-1173
[6]Bissada NF.Symptomatology and clinical features of hypersensitive teeth[J].Arch Oral Biol,1994,39(suppl):31-
[7]Brannstrom M.Sensitivity of dentine[J].Oral Surg Oral Med Oral Pathol,1966,21:517-26
[8] Schmidlin PR,Sahrmann P.Current management of dentin hypersensitivity. Clin Oral Invest.2013 Mar,17(Suppl 1):S55–S59
[9]Orchardson R,Gillam DG .The efficacy of potassium salts as agents for treating dentin hypersensitivity. J Orofac Pain.2000,14(1):9–1
[10]Poulsen S,Errboe M,Hovgaard O,Worthington HW .Potassium nitrate toothpaste for dentine hypersensitivity. Cochrane Database Syst Rev .2001;(2): CD001476
[11]Du Min Q,Bian Z,Jiang H,Greenspan DC,Burwell AK,Zhong Jet al.Clinical evaluation of a dentifrice containing calcium sodium phosphosilicate (novamin) for the treatment of dentin hypersensitivity[J].Am J Dent,2008,21(4):210-214
[12]Mehta AB,Kumari V,Jose R et al.Remineralization potential of bioactive glass and casein phosphopeptide-amorphous calcium phosphate on initial carious lesion: An in-vitro pH-cycling study. J Conserv Dent. 2014 Jan;17(1):3-7
[13] Hench LL,Jones JR .Bioactive glasses: Frontiers and Challenges. Front Bioeng Biotechnol. 2015 Nov 30;3:194
[14]Nithin Manchery Gopinath1,Joseph John2,et al.Journal of International Oral Health 2015; 7(8):118-122
[15]Acharya AB,Surve SM,Thakur SL.Aclinical study of the effect of calcium sodium phosphosilicate on dentin hypersensitivity[J].J Clin Exp Dent,2013,5(1):-
[16]Litkowski L,Greenspan DC.Aclinical study of the effect of calcium sodium phosphosilicate on dentin hypersensitivity – Proof of principle[J].JClin Dent,2010,21(3):77-81
[17]Bennett T.Amaechi Sapna M. Mathews Poornima K. Mensinkai. Effect of theobromine-containing toothpaste on dentin tubule occlusion in situ. Clin Oral Invest (2015) 19:109–116
[18]DANIEL RODRIGUES DE ABREU,ROBSONTETSUO SASAKI et al(2011),Effect of Home-Use and In-Office Bleaching Agents Containing Hydrogen Peroxide Associated with Amorphous Calcium Phosphate on Enamel Microhardness and Surface Roughness.J Esthet Restor Dent.2011. 23 (3): 158–168