MMP-9和VEGF在腮腺多形性腺瘤中的表达研究

›› 2015, Vol. 35 ›› Issue (4): 258-261.

MMP-9和VEGF在腮腺多形性腺瘤中的表达研究

程晗菲,尹林,孟令旭

南京医科大学第一附属医院

收稿日期:2014-09-05 修回日期:2014-12-03 出版日期:2015-04-28 发布日期:2015-04-27
通讯作者: 程晗菲 E-mail:173179664@qq.com
基金资助:
江苏省教育厅自然科学基金

Expression of MMP9 and VEGF in parotid gland pleomorphic adenoma

Received:2014-09-05 Revised:2014-12-03 Online:2015-04-28 Published:2015-04-27
Contact: Han-fei CHENG E-mail:173179664@qq.com

摘要/Abstract

摘要： 目的研究MMP-9和VEGF在腮腺多形性腺瘤中的表达情况及其与肿瘤良恶性的关系。方法应用荧光实时定量PCR和Western blotting方法检测32对多形性腺瘤及其瘤旁组织中MMP-9和VEGF的表达。结果 32例多形性腺瘤中26例为良性，6例为恶性。MMP-9和VEGF的mRNA和蛋白表达量在多形性腺瘤组织中高于瘤旁组织（P＜0.05）；在恶性多形性腺瘤组织中高于良性多形性腺瘤组织（P＜0.05）。结论 MMP-9和VEGF在腮腺多形性腺瘤中高表达，并且可能与腮腺多形性腺瘤的恶变相关；腮腺多形性腺瘤肿瘤外1 cm为安全切除界限。

关键词: 多形性腺瘤, 基质金属蛋白酶-9, 血管内皮生长因子

Abstract: Objective To investigate the relationship between the expressions of MMP9 and VEGF in parotid pleomorphic adenoma,benign pleomorphic adenoma and malignant pleomorphic adenoma.Methods 　 Realtime fluorescent quantitative PCR and Western Blotting methods were used to detect the expression of MMP9 and VEGF in 32 pairs of pleomorphic adenoma tissue and the tissue beside the tumor.Results 　26 out of 32 cases were benign pleomorphic adenoma,and other 6 were malignant.The mRNA and protein expression rate of MMP9 and VEGF in pleomorphic adenoma tissues were higher than those in the tissue beside the tumor(P＜0.05).The mRNA and protein expression rate of MMP9 and VEGF in malignant pleomorphic adenoma tissues were higher than those in benign pleomorphic adenoma tissues(P＜0.05).Conclusions 　 MMP9 and VEGF are highly expressed in parotid pleomorphic adenoma,and MMP9 and VEGF may be related with malignant pleomorphic adenoma.Taking 1.0 cm away from parotid pleomorphic adenoma as a boundary is safe for the excision of parotid pleomorphic adenoma.

Key words: pleomorphic adenoma, MMP9, VEGF

中图分类号:

　R739.87

程晗菲尹林孟令旭. MMP-9和VEGF在腮腺多形性腺瘤中的表达研究[J]. 口腔医学, 2015, 35(4): 258-261.

参考文献

[1]Seifert G,Sobin LH.The World Health Organization′ Histological classification of salivary gland tumors[J].Cancer,1992,70(2):379-385
[2]Vandooren J,Van den Steen PE,Opdenakker G.Biochemistry and molecular biology of gelatinase B or matrix metalloproteinase9 (MMP9):the next decade[J].Crit Rev Biochem Mol Biol,2013,48(3):222-72
[3]赵坚，张望群，李金源，等.VEGF、NFκB/p50在不同分型涎腺多形性腺瘤中的表达及意义[J].军医进修学院学报,2011,32(5):516-518
[4]Harada H.Histomorphological investigation regarding to malignant transformation of pleomorphic adenoma (socalled malignant mixed tumor) of salivary gland origin:special reference to carcinoma[J].Kurume Mcd,2000,47(4):307-323
[5]张学斌,杨文军,刘应时,等.细胞核增值抗原几血管内皮生长因子在多形性腺瘤中表达的相关性研究[J].中华临床医师杂志,2011,05(9):2728-2730
[6]Groblewska M,Siewko M,Mroczko B,et al.The role of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) in the development of esophageal cancer[J].Folia Histochem Cytobiol,2012,50(1):12-19
[7]Yang X,Zhang P,Ma Q,et al.EMMPRIN contributes to the in vitro invasion of human salivary adenoid cystic carcinoma cells[J].Oncol Rep,2012,27(4):1123-1127
[8]Vilen ST,Salo T,Sorsa T,et al.Fluctuating roles of matrix metalloproteinase9 in oral squamous cell carcinoma[J].Scientific World Journal,2013,2013:920595-
[9]Omran OM,Thabet M.Gelatinases A and B expression in human colorectal cancer in upper egypt:a clinicopathological study[J].Ultrastruct Pathol,2012,36(2):108-116
[10]Yüce I,Bayram A,Call S,et al.The role of CD44 and matrix metalloproteinase9 expression in predicting neck metastasis of supraglottic laryngeal carcinoma[J].Am J Otolaryngol,2011,32(2):141-146
[11]Morris SF,O′Hanlon DM,McLaughlin R,et al.The prognostic significance of CD44s and CD44v6 expression in stage two breast carcinoma:an immunohistochemical study[J].Sturg Oncol,2001,27(6):527-531
[12]Ribatti D.The crucial role of vascular permeability factor/vascular endothelial growth factor in angiogenesis：a historical review[J].Br J Haematol,2005,128(3):303-309
[13]杨重恒，赵宝东.腮腺多形性腺瘤中的VEGF表达及其意义[J].辽宁医学院学报,2007,28(2):10-12
[14]Kondo S,Mukudai Y,Soqa D,et al.Differential expression of vascular endothelial growth factor in high and lowmetastasis cell lines of salivary gland adenoid cystic carcinoma[J].Anticancer Res,2014,34(2):671-677
[15]Borase AP,Ganvir SM,Hazarey VK,et al.Estimation of vascular endothelial growth factor gene460 C/T polymorphism as a biomarker in oral squamous cell carcinoma patients from the Indian subcontinent［J/OL］.J Investiq Clin Dent,2014［2014-11-14］doi:10.1111/jicd.12103.
[16]Li M,Wang Z,Xing Y,et al.A multicenter study on expressions of vascular endothelial growth factor,matrix metallopeptidase9 and t3 inhibitor of metalloproteinase2 in oral and maxillofacial squamous cell carcinoma[J].Iran Red Crescent Med,2014,16(3):e13185-
[17]Liang L,Li HJ,Zhang YR,et al.Expressions of VEGF,MMP9 and Ki67 in salivary gland tumor[J].Pract Stomatol,2008,24(5):728-732
[18]邱嘉旋,朱声荣,黄松,等，腮腺多形性腺瘤并腺体区域性切除边界的界定［J］.临床口腔医学杂志，2008,24（1）:30-31.