PLGA/HA/生物活性玻璃复合膜的制备及抗菌性能研究

摘要/Abstract

摘要： 目的　通过对PLGA/HA/生物活性玻璃复合膜的制备和检测，探索一种便于牙周临床使用的缓释杀菌体系。方法：采用溶剂挥发法制备PLGA/HA/生物活性玻璃复合膜。实验中各组PLGA/HA载体相同，向其中添加不同质量的BAG粉末，测量复合膜的厚度和表面微观结构变化情况。提取复合膜以及与复合膜中BAG等量的粉末的浸提液，测量浸提过程中离子释放、复合膜质量损失、浸提液酸碱性变化情况，以牙周膜成纤维细胞为实验对象测试浸提液的细胞毒性水平；并以MTT法测试浸提液对牙龈卟啉单胞菌（P.g.）和伴放线放线杆菌（A.a.）的杀菌作用。结果：在复合膜体系中，BAG的释放速度受到控制；较之BAG粉末，复合膜对P.g.和A.a.的杀灭作用更持久。结论：PLGA/HA/生物活性玻璃复合膜有望成为一种局部使用的牙周炎缓释药物。

关键词: 聚乳酸-羟基乙酸, 透明质酸, 生物活性玻璃（BAG）, 复合膜, 抗菌

Abstract: Objective　 To fabricate and assess the PLGA/HA/BAG composite membranes as a brand-new control-releasing delivery system in treatment of periodontitis. Methods The PLGA/HA/BAG composite films were synthesized by a solvent-evaporation method. SEM was utilized for the morphology and mass loss during the immersion, ion release and changes in pH were also analyzed. The immersion of the films and pristine BAG powder were extracted, between which the efficiency of antibacterial effect was compared with MTT assay. CCK-8 kit was employed to evaluate the cytotoxicity with human periodontal ligament fibroblast cells. Results The film had longer antibacterial effects against Porphyromonas gingivalis (ATCC 33277) and Actinobacillus actinomycetemcomitans (ATCC 29523) than powder. Conclusion The film has great potentials of being a treatment for periodontitis in the future.

Key words: PLGA, hyaluronic acid, bioactive glass, composite film, antibacterial

中图分类号:

R783.1

许晓马骞陈亚明. PLGA/HA/生物活性玻璃复合膜的制备及抗菌性能研究[J]. , 2018, 38(7): 606-611.

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