脂肪干细胞复合成骨性干细胞膜片用于修复兔颅骨缺损的实验研究

›› 2018, Vol. 38 ›› Issue (7): 0-0.

• 基础研究 • 下一篇

脂肪干细胞复合成骨性干细胞膜片用于修复兔颅骨缺损的实验研究

王之发¹,孙硕辉¹,马军利²,王桥¹,汪维健¹,段建民²,³

1. 中国人民解放军广州总医院口腔科
2. 广州军区广州总医院
3.

收稿日期:2018-01-24 修回日期:2018-04-16 出版日期:2018-07-28 发布日期:2018-07-28
通讯作者: 王之发 E-mail:wangzf19871014@163.com
基金资助:
国家自然科学基金;广东省自然基金;军队后勤科研计划重点项目

An experimental study of the application of adipose-derived stem cells compositing osteoblastic mesenchymal stem cell sheets to improveing bone formation in calvarial defects in rabbits

Received:2018-01-24 Revised:2018-04-16 Online:2018-07-28 Published:2018-07-28
Contact: Zhi-Fa WANG E-mail:wangzf19871014@163.com

摘要/Abstract

摘要： 目的　细胞膜片技术已经被证实了可以成功用于多种组织再生，我们前期实验证明了脂肪干细胞（ADSCs）可以显著促进成骨性细胞膜片（BMSCs sheet）的异位成骨能力。因此，本实验的目的是验证ADSCs能否促进成骨性BMSCs膜片的骨缺损修复能力。方法：ADSCs和BMSCs分别取自同一个供体的腹股沟和髂骨骨松质，通过连续成骨诱导的方法制备成骨性BMSCs膜片，并分析BMSCs膜片的特性；然后将ADSCs复合在BMSCs膜片上，构架ADSCs-BMSCs膜片复合体，移植到兔颅骨缺损模型，单纯BMSCs膜片和颅骨缺损组作为对照。移植八周后，Micro-CT扫描和组织学分析其骨修复再生情况。结果：BMSCs膜片由含有大量矿化结节的多层细胞膜片构成；移植八周后，相比于对照组，实验组新生组织骨密度更高，新生矿化组织量也更多，同时新生骨组织/总新生组织体积（BV/TV）的比值也更高，表明ADSCs显著提高了BMSCs膜片的骨缺损修复能力。结论：ADSCs复合BMSCs膜片可以明显促进兔颅骨缺损的骨再生修复，该方法为促进骨修复重建提供了一种新的方法。

关键词: 脂肪干细胞, 细胞膜片, 颅骨缺损, 骨再生

Abstract: Objective　Cell sheet technology has been proved successful for multiple tissue regeneration. Our previous study demonstrated that adipose-derived stem cells (ADSCs) can significantly promote the ectopic osteogenic potential of osteoblastic bone marrow mesenchymal stem cell (BMSCs) sheet. Therefore, the purpose of this study was to verify whether ADSCs can promote bony healing potential of BMSCs sheet in calvarial defects in rabbits. Methods ADSCs and BMSCs were obtained from the same donor's inguinal fat and iliac cancellous bone, respectively. BMSCs were cultivated by continuous osteoinduction method for two weeks, and the characteristics of BMSCs sheet were analyzed. Then the combination of ADSCs and BMSCs sheet was transplanted to calvarial defects in rabbits. BMSCs sheet and calvarial defects without any treatment were considered as control groups. Eight weeks after the transplantation, the bony restoration and regeneration were assessed by microcomputed tomography (Micro-CT) scanning and histological analysis. Results BMSCs sheet consisted of multilayer sheets containing a large number of mineralized calcium nodules. After eight weeks of transplantation, compared to control groups, BMSCs sheet with ADSCs increased better bone regeneration as evidenced by the greater density of bone, larger number of newly formed mineralized tissue and greater bone volume / total volume (BV / TV) ratio within the composite constructs. Conclusions The combination of ADSCs and BMSCs sheet can significantly promote bone regeneration in calvarial defects in rabbits, which provides a new way to improve bone healing.

Key words: Adipose-derived stem cells, Cell sheet, Calvarial defects, Bone regeneration

中图分类号:

　R329.28

王之发孙硕辉马军利王桥汪维健段建民. 脂肪干细胞复合成骨性干细胞膜片用于修复兔颅骨缺损的实验研究[J]. , 2018, 38(7): 0-0.

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脂肪干细胞复合成骨性干细胞膜片用于修复兔颅骨缺损的实验研究

An experimental study of the application of adipose-derived stem cells compositing osteoblastic mesenchymal stem cell sheets to improveing bone formation in calvarial defects in rabbits

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