牙龈卟啉单胞菌对db/db小鼠骨髓来源巨噬细胞极化作用的初步探究

›› 2020, Vol. 40 ›› Issue (1): 13-16.

牙龈卟啉单胞菌对db/db小鼠骨髓来源巨噬细胞极化作用的初步探究

杨亚楠¹,于时卉²,闫香珍¹,罗礼君¹

1. 同济大学附属口腔医院
2. 山东省平度市人民医院

收稿日期:2019-07-22 修回日期:2019-09-03 出版日期:2020-01-28 发布日期:2020-01-16
通讯作者: 罗礼君 E-mail:juneluo@yeah.net
基金资助:
国家自然科学基金;上海市卫计委自然基金

A preliminary study of polarization of bone marrow-derived macrophages stimulated by porphyromonas gingivalis in db/db mice

Received:2019-07-22 Revised:2019-09-03 Online:2020-01-28 Published:2020-01-16

摘要/Abstract

摘要： 目的初步探讨牙龈卟啉单胞菌（Pg）对db/db小鼠骨髓来源的巨噬细胞（BMDMs）极化的影响。方法以Pg刺激野生型C57BL/6J及db/db小鼠BMDMs，检测M1、M2基因标志物的表达水平。采用qRT-PCR法分别检测Tnf-α、Il-12以及Arg1、Ym1、Fizz1和Mrc1的基因相对表达量。结果与野生型相比，在IL-4诱导下，Pg感染与否对db/db小鼠BMDMs表达基因Arg1、Fizz1和Mrc1的影响无统计学差异；db/db小鼠BMDMs中Ym1的表达明显下降，M1极化标志基因Tnf-α无明显差异，Il-12的表达显著降低。结论在db/db小鼠中Pg诱导M2向M1转变的促进作用减弱。

关键词: ［关键词］牙周炎, 牙龈卟啉单胞菌, 2型糖尿病, 巨噬细胞极化

Abstract: Objective To investigate the effects of porphyromonas gingivalis (Pg) on polarization of bone marrow-derived macrophages (BMDMs) in db/db mice. Methods Pg was used to stimulate BMDMs from wild type C57BL / 6 or db/db mice, the expression level of M1 and M2 gene markers was detected. The relative expression levels of Tnf-α, Il-12, Arg1, Ym1, Fizz1 and Mrc1 were detected by qRT-PCR. Results Compared with wild type mice, there was no statistically significant difference in the effect of Pg infection or not on BMDMs expressing Arg1, Fizz1 and Mrc1 in db/db mice under IL-4 induction; however, the expression of Ym1 in BMDMs of db/db mice was significantly decreased, while no significant difference was found in Tnf-α, and the expression of il-12 was markedly decreased. Conclusion The ability for Pg to switch from M2 to M1 was weakened in BMDMs of db/db mice.

Key words: Key words: periodontitis, porphyromonas gingivalis, type 2 diabetes mellitus, macrophage polarization

中图分类号:

R781.42

杨亚楠于时卉闫香珍罗礼君. 牙龈卟啉单胞菌对db/db小鼠骨髓来源巨噬细胞极化作用的初步探究[J]. 口腔医学, 2020, 40(1): 13-16.

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