核受体共激活因子7在槟榔碱诱导的上皮间充质转化中的生物学功能

›› 2020, Vol. 40 ›› Issue (1): 43-47.

核受体共激活因子7在槟榔碱诱导的上皮间充质转化中的生物学功能

刘清辉,何黎明

长沙市口腔医院

收稿日期:2018-10-19 修回日期:2018-12-29 出版日期:2020-01-28 发布日期:2020-01-16
通讯作者: 何黎明 E-mail:liming1985420@qq.com
作者简介:2018-11-29

The biological functions of NCOA7 mediated EMT in oral submucous fibrosis

Received:2018-10-19 Revised:2018-12-29 Online:2020-01-28 Published:2020-01-16

摘要/Abstract

摘要： 目的研究NCOA7在口腔黏膜下纤维性变（OSF）中的表达及意义，并探讨其在口腔黏膜角质细胞株HOKs间充质转化过程中的作用。方法收集OSF组织30例及正常口腔黏膜组织15例，采用免疫印迹法检测NCOA7、上皮间充质转化相关标记物在OSF组织及正常黏膜组织中蛋白水平的表达情况；以western blot法检测槟榔碱刺激后HOKs中NCOA7、上皮及间充质标记物的蛋白水平变化。结果 NCOA7在30例OSF组织高表达（P<0.05）；同时在细胞基底层出现间充质标记物的阳性表达。NCOA7在槟榔碱刺激的HOKs中高表达,且具有槟榔碱浓度依赖性（P=0.0043）；随着槟榔碱浓度的升高，上皮标志物表达逐渐下调，而间充质标记物表达逐渐升高。结论口腔黏膜下纤维性变组织中高表达的NCOA7，可能参与调控上皮细胞间充质转化过程，进一步促进纤维化进程。

关键词: 口腔黏膜下纤维性变, NCOA7, 上皮间充质转化, 槟榔碱, 人口腔角质细胞

Abstract: Objective To investigate the expression of nuclear receptor coactivator 7 (NCOA7) in oral submucous fibrosis (OSF) and explore its effect on epithelial to mesenchymal transformation (EMT). Methods 30 cases of OSF tissues, and 15 cases of normal tissues were collected. The protein expression level of NCOA7 and Vimentin in OSF tissues was determined by immunohistochemical (IHC) method. NCOA7 protein and epithelial-mesenchymal transformation related proteins (E-cadherin, Vimentin) in arecoline-treated HOKs were detected by western blot. Results NCOA7 was highly expressed (P<0.05) in OSF tissues at protein level compared to normal oral mucosa. Epithelial basal cells showed a higher vimentin expression in OSF tissues. After 24 hours of treatment with different arecoline concentration, aberrant expression of NCOA7, E-cadherin, vimentin and α-SMA were found in a dose-depended manner. Conclusion Overexpression of NCOA7 was found in OSF tissues and arecoline stimulated epithelial cells and EMT process was found play a role in the pathogenesis of OSF. NCOA7 might be a new therapeutic target for OSF.

Key words: Oral submucous fibrosis, Nuclear receptor coactivator 7 (NCOA7), Epithelial to mesenchymal transformation (EMT), Arecoline, Human oral keratinocytes (HOKs)

中图分类号:

R781.59

刘清辉何黎明. 核受体共激活因子7在槟榔碱诱导的上皮间充质转化中的生物学功能[J]. 口腔医学, 2020, 40(1): 43-47.

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