青皮对主要致龋细菌生长的影响及网络药理学分析其防龋机制

›› 2020, Vol. 40 ›› Issue (2): 101-107.

青皮对主要致龋细菌生长的影响及网络药理学分析其防龋机制

吴泽钰¹,薛瑞²,袁曦玉¹,从兆霞¹,赵今³

1. 新疆医科大学附属口腔医（学）院牙体牙髓科，新疆乌鲁木齐（830054）
2. 新疆医科大学附属口腔医（学）
3. 新疆医科大学第一附属医院

收稿日期:2019-08-21 修回日期:2019-10-12 出版日期:2020-02-28 发布日期:2020-02-20
通讯作者: 赵今 E-mail:merryljin@sina.com
基金资助:
哈萨克族肥胖儿童口腔与肠道微生物群落相关性研究

Effect of Citrus reticulate on the growth of main cariogenic bacteria and its pharmacological analysis of its anti-caries mechanism

Received:2019-08-21 Revised:2019-10-12 Online:2020-02-28 Published:2020-02-20

摘要/Abstract

摘要： ［摘要］　目的　研究青皮提取物对6种主要致龋菌生长的影响，基于网络药理学方法探讨青皮潜在的防龋机制。方法　采用液体稀释法测定6种主要致龋菌MIC和MBC。基于TCMSP数据库获取青皮化学成分及其对应靶点，通过Gene Cards、OMIM数据库获取龋病靶点，将药物与疾病靶点映射得到青皮化学成分的预测靶点。构建成分-靶点调控网络及蛋白-蛋白相互作用网络，利用DAVID数据库分析预测靶点的GO富集分析，利用Kobas数据库分析预测靶点的KEGG富集分析。结果　青皮提物对变形链球菌、血链球菌、远缘链球菌、黏性放线菌、内氏放线菌和嗜酸乳杆菌MIC分别为1, 0.5, 1, 2, 2, 2mg/mL，MBC分别为2, 1, 2, 4, 4, 4mg/mL。网络药理学方法得到青皮化学成分12个，靶点28个，关键化学成分包括新橙皮苷、柚皮素、川陈皮素等，关键靶点包括TNF、CAT、MMP9等。KEGG富集分析得到AGE-RAGE、NF-κB等信号通路。结论　青皮提取物对6种主要致龋细菌生长具有显著抑制作用，网络药理学揭示了其化学成分防治龋病的药理基础及作用机制，能为后续从青皮中开发防龋药物提供实验和理论依据。

关键词: 青皮, 致龋细菌, 网络药理学, 靶点, 作用机制

Abstract: Abstract：　Objective　To study the effects of Qingpi extract on the growth of six major cariogenic bacteria, and to explore the potential anti-caries mechanism of Qingpi based on network pharmacology. Methods　The MIC and MBC of six major cariogenic bacteria were determined by liquid dilution method. Based on TCMSP, the chemical constituents and corresponding targets of Qingpi were obtained. The related targets of caries were obtained through Gene Cards and OMIM database. The chemical constituents of Qingpi were mapped to disease targets to predict caries. Using Cytoscape software to construct a component-target regulatory network and protein-protein interaction network, use the DAVID database to analyze the GO classification enrichment analysis, and use the Kobas database to analyze the target KEGG signal path analysis. Results　The MIC of Qingpi against S. mutans, S. sanguis, S. sobrinus, A. viscosus, A. naeslundii and L. acidophilus was 1, 0.5, 1, 2, 2, 2mg/mL, MBC was 2, 1, 2, 4, 4, 4mg/mL．According to the results of network pharmacology, there are 12 effective components for the treatment of caries and 28 targets. The key active ingredients include neohesperidin, naringenin and nobiletin. The key targets include TNF, CAT, MMP9 et al. Pathway enrichment analysis showed signal pathways such as AGE-RAGE、NF-κB signaling pathway. Conclusions　The extract of Qingpi has an inhibitory effect on the growth of six major cariogenic bacteria, and network pharmacology reveals the pharmacological basis and mechanism of its chemical constituents against caries. It can provide experimental and theoretical basis for the subsequent development of drugs for preventing caries from the Qingpi.

Key words: Citrus reticulate, cariogenic bacteria, network pharmacology, target, mechanism of action

中图分类号:

R965.2

吴泽钰薛瑞袁曦玉从兆霞赵今. 青皮对主要致龋细菌生长的影响及网络药理学分析其防龋机制[J]. 口腔医学, 2020, 40(2): 101-107.

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