VEGF和PDGF联合诱导的骨髓间充质干细胞膜片复合马鹿角粉/PVA支架的体内成血管相关研究

›› 2021, Vol. 41 ›› Issue (10): 872-877.

VEGF和PDGF联合诱导的骨髓间充质干细胞膜片复合马鹿角粉/PVA支架的体内成血管相关研究

裴培¹,周琦琪²,赵小琦²,韩祥祯²

1. 新疆医科大学第一附属医院
2. 新疆医科大学第一附属医院口腔修复科

收稿日期:2021-04-21 修回日期:2021-06-25 出版日期:2021-10-28 发布日期:2021-10-29
通讯作者: 裴培 E-mail:6449217@qq.com
基金资助:
国家自然科学基金资助项目

In vivo study of vascularization of bone marrow mesenchymal stem cell membrane combined with red antler powder/PVA scaffold induced by VEGF and PDGF

Received:2021-04-21 Revised:2021-06-25 Online:2021-10-28 Published:2021-10-29
Contact: Pei PEI E-mail:6449217@qq.com
Supported by:
National Science Fund subsidized project

摘要/Abstract

摘要： 目的探讨联合应用VEGF和PDGF诱导的细胞膜片-马鹿角粉/PVA支架回植动物体内后，其在体内的可行性及相关成血管作用。方法选取36只10周龄的雄性SD大鼠,体重控制在250±30g，随机分为3组,每组12只。将3D打印的马鹿角粉/PVA支架回植大鼠肾被膜后进行大体观察、HE染色、RT-PCR。回植动物时分为3组，实验组：生长因子诱导骨髓干细胞膜片-支架复合物；对照组：骨髓干细胞膜片-支架复合物；空白组：造模后不移植任何材料。结果经回植取材后实验组中成血管相关因子Ang-1（血管生成素-1）、HIF-1-α（低氧诱导因子-1-α）、HGF（肝细胞生长因子）和IGF-1（胰岛素样生长因子-1）的表达增高（p＜0.05），HE染色中实验组可见有更多的新生血管生成。结论 VEGF与PDGF联合诱导的骨髓间充质干细胞膜片-马鹿角粉支架有效促进新血管的生成,有助于组织工程骨的血管化。

关键词: VEGF, PDGF, 成血管, 细胞膜片, 组织工程

Abstract: Objective To explore SD rat bone marrow mesenchymal stem cells (BMSCs) induced by combined application of vascular endothelial growth factor (VEGF) and plateletderived growth factorBB (PDGFBB); to explore its feasibility in vivo and the expression of related angiogenesis, after bone marrow mesenchymal stem cells cell membranered antler powder/PVA scaffold is replanted in animals. Methods Thirtysix 10weekold male SD rats were selected and their weight was controlled at (250±30)g, and they were randomly divided into 3 groups, each with 12 rats. The 3D printed red deer antler powder/PVA scaffold was replanted into the rat kidney capsule, and then gross observation, HE staining, blood vessel count and RTPCR were performed. The animals were classified into 3 groups, experimental group:growth factor induced BMSCs patchstent complex; control group:BMSCs patchstent complex; blank group:no materials were transplanted after modeling. Results After replanting, the expression of factors related to angiogenesis, hypoxia inducible factor1α (hypoxia inducible factor1α, HIF1α), angiopoietin1 (angiopoietin1, Ang1), hepatocyte growth factor (HGF) and insulinlike growth factor1 (IGF1), were all increased (P＜0.05) in experimental group. The HE staining showed obvious angiogenesis in the experimental group. The number of blood vessels in the experimental group was higher than that in the control group. Conclusion The BMSCs membranered antler powder scaffold induced by VEGF and PDGFBB can effectively promote the formation of new blood vessels and contribute to the vascularization of tissue engineered bone.

Key words: VEGF, PDGF, angiogenesis, cell membrane, tissue engineering

中图分类号:

R783.1

裴培周琦琪赵小琦韩祥祯. VEGF和PDGF联合诱导的骨髓间充质干细胞膜片复合马鹿角粉/PVA支架的体内成血管相关研究[J]. 口腔医学, 2021, 41(10): 872-877.

参考文献 20

[1]	Tian T, Zhang T, Lin Y, et al.Vascularization in craniofacial bone tissue engineering[J].J Dent Res, 2018, 97(9):969-976
[2]	Chan SJ, Esposito E, Hayakawa K, et al.Vascular endothelial growth factor 165-binding heparan sulfate promotes functional recovery from cerebral ischemia[J].Stroke, 2020, 51(9):2844-2853
[3]	Liu WW, Li XK, Jiao YL, et al.Biological effects of a three-dimensionally printed Ti6Al4V scaffold coated with piezoelectric BaTiO3 nanoparticles on bone formation[J].ACS Appl Mater Interfaces, 2020, 12(46):51885-51903
[4]	毕翔宇, 孙安, 何惠宇.联合-和-促进骨髓间充质干细胞血管化的体内研究[J].口腔医学, 2020, 40(1):7-12
[5]	赵小琦, 丁刘闯, 韩祥祯, 等.打印鹿角粉聚乙烯醇支架与纳米级羟基磷灰石聚乙烯醇支架的性能比较[J].口腔医学研究, 2018, 34(9):1011-1015
[6]	熊美萍, 贾高智, 袁广银.全降解镁合金组织工程支架孔隙特征与性能关系研究[J].口腔医学, 2020, 40(11):971-975
[7]	倪王成, 胡琳驰, 张维丹, 等.打印和氧化锆种植体骨结合性能的动物实验评价[J].口腔医学, 2021, 41(2):144-148
[8]	Shi R, Huang YL, Ma C, et al.Current advances for bone regeneration based on tissue engineering strategies[J].Front Med, 2019, 13(2):160-188
[9]	Tang GK, Tan ZH, Zeng WS, et al.Recent advances of chitosan-based injectable hydrogels for bone and dental tissue regeneration[J]. Front Bioeng Biotechnol, 2020, 8: 587658.
[10]	de Witte TM, Wagner AM, Fratila-Apachitei LE, et al.Degradable poly(methyl methacrylate)-co-methacrylic acid nanoparticles for controlled delivery of growth factors for bone regeneration[J].Tissue Eng Part A, 2020, 26(2324):1226-1242
[11]	刘小元, 张凯, 韩祥祯, 等.打印复合骨组织工程支架研究现状[J].口腔疾病防治, 2020, 28(1):52-55
[12]	魏婷.打印辅助手术治疗下颌骨缺损疗效的分析[J].口腔医学, 2020, 40(3):232-238
[13]	Zhang RJ, Liu Y, Qi YQ, et al.Self-assembled peptide hydrogel scaffolds with VEGF and BMP-2 enhanced in vitro angiogenesis and osteogenesis[J]. Oral Dis, 2021: span.
[14]	Lu WY, Xu W, Li JJ, et al.Effects of vascular endothelial growth factor and insulin growth factor?1 on proliferation,migration,osteogenesis and vascularization of human carious dental pulp stem cells[J].Mol Med Rep, 2019, 20(4):3924-3932
[15]	Niu Y, Li Q, Ding Y, et al.Engineered delivery strategies for enhanced control of growth factor activities in wound healing[J]. Adv Drug Deliv Rev, 2019, 146: 190-208.
[16]	Shen Z, Chen ZH, Li ZG, et al.Total flavonoids of rhizoma drynariae enhances angiogenic-osteogenic coupling during distraction osteogenesis by promoting type H vessel formation through PDGF-BB/PDGFR-β instead of HIF-1α/ VEGF axis[J]. Front Pharmacol, 2020, 11: 503524.
[17]	Zhao N, Suzuki A, Zhang XL, et al.Dual aptamer-functionalized in situ injectable fibrin hydrogel for promotion of angiogenesis via codelivery of vascular endothelial growth factor and platelet-derived growth factor-BB[J].ACS Appl Mater Interfaces, 2019, 11(20):18123-18132
[18]	Dinh ND, Kukumberg M, Nguyen AT, et al.Functional reservoir microcapsules generated via microfluidic fabrication for long-term cardiovascular therapeutics[J].Lab Chip, 2020, 20(15):2756-2764
[19]	Shi Y, Liu Z, Zhang Q, et al.Phosphorylation of seryl-tRNA synthetase by ATMATR is essential for hypoxia-induced angiogenesis[J].PLoS Biol, 2020, 18(12):e3000991-
[20]	Macías-Pérez JR, Vázquez-López BJ, Mu?oz-Ortega MH, et al.Curcumin and α/β-adrenergic antagonists cotreatment reverse liver cirrhosis in hamsters: Participation of nrf-2 and NF-κB[J]. J Immunol Res, 2019, 2019: 3019794.