口腔医学 ›› 2023, Vol. 43 ›› Issue (1): 52-56.doi: 10.13591/j.cnki.kqyx.2023.01.009

• 基础研究 • 上一篇    下一篇

超声处理对牙本质玷污层性状及粘接强度的影响

王如玉1,2,潘雅慧1,聂蓉蓉1(),孟翔峰3()   

  1. 1 南京大学医学院附属口腔医院,南京市口腔医院修复科,江苏南京(210008)
    2 南京大学医学院附属口腔医院,南京市口腔医院中心实验室,江苏南京(210008)
    3 南京大学医学院附属口腔医院,南京市口腔医院修复工艺科,江苏南京(210008)
  • 修回日期:2022-08-15 出版日期:2023-01-28 发布日期:2023-01-11
  • 通讯作者: 聂蓉蓉, E-mail: nrr63@nju.edu.cn; 孟翔峰, E-mail: mengsoar@nju.edu.cn。
  • 基金资助:
    南京市医学科技发展一般性课题(YKK17142)

Studies on the effect of ultrasound on properties and bonding strength of dentin smear layer

WANG Ruyu1,2,PAN Yahui1,NIE Rongrong1(),MENG Xiangfeng3()   

  1. Department of Prosthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China
  • Revised:2022-08-15 Online:2023-01-28 Published:2023-01-11
  • Contact: NIE Rongrong,MENG Xiangfeng

摘要: 目的 探究超声处理对牙本质表面玷污层性状及其对通用型树脂粘接剂自酸蚀模式下粘接强度的影响。方法 将40颗人无龋下颌第三磨牙随机分为320目碳化硅砂纸打磨组以及砂纸打磨加超声处理组,运用扫描电子显微镜观察牙本质表面玷污层性状;处理后的牙体分别用Rhodamine B染色的2种通用型树脂粘接剂(Clearfil Universal Bond和All-Bond Universal, pH值分别为2.3和3.1)粘接复合树脂,观察粘接界面的渗透情况;最后制作微拉伸试件进行微拉伸强度测试。结果 扫描电子显微镜显示,超声处理能够使大量牙本质小管暴露;激光扫描共聚焦显微镜显示超声处理过后2种粘接剂都能沿牙本质小管渗透入牙本质深部;微拉伸强度测试结果表明超声处理能够增强2种通用型树脂粘接剂自酸蚀模式下的粘接强度;但在相同的牙本质表面处理条件下,这两种粘接剂间的粘接强度差异无统计学意义。结论 超声处理能够部分去除牙本质表面玷污层,暴露牙本质小管,增加通用型树脂粘接剂自酸蚀模式下的粘接强度。

关键词: 超声, 牙本质, 玷污层, 通用型树脂粘接剂, 微拉伸强度

Abstract: Objective To explore the effect of ultrasound on dentin smear layer's surface and bonding strength of the universal resin adhesive under self-etching mode. Methods Forty mandibular third molars without caries were randomly divided into two groups; one was polished with silicon carbide sandpaper; the other was polished with silicon carbide sandpaper followed by ultrasonic treatment. Scanning electron microscope (SEM) was used to observe surface of the dentin. Treated teeth were bonded with two universal resin adhesives, Clearfil Universal Bond (pH=2.3) and All-Bond Universal (pH=3.1), and the penetration of the bonding interface was observed with a confocal laser scanning microscope (CLSM) after Rhodamine B staining. Finally, the micro tensile bond strength test was conducted to test the adhesion. Results The SEM showed that after polishing with silicon carbide sandpaper, the smear layer of the dentin surface was scratched, and dentin tubules were almost completely blocked, with no obvious dentin tubules exposed. After ultrasonic treatment, the scratches were reduced, and a large number of dentin tubules were exposed. CLSM showed that both adhesives could penetrate the dentin along the dentin tubules more deeply after ultrasound treatment. Micro tensile bond strength tests showed that ultrasonic treatment could enhance the bonding strength of two universal resin adhesives. However, there was no statistical difference in bonding strength between these two universal resin adhesives under the same treatment. Conclusion Ultrasound can partially remove the smear layer on dentin's surface, expose dentin tubules, and increase universal resin adhesives' penetration depth and bonding strength under self-etching mode.

Key words: ultrasound, dentin, smear layer, universal resin adhesive, micro tensile bond strength

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