PAAc-NHS酯/明胶复合水凝胶口腔贴剂的性能研究

doi:10.13591/j.cnki.kqyx.2023.04.003

口腔医学 ›› 2023, Vol. 43 ›› Issue (4): 301-307.doi: 10.13591/j.cnki.kqyx.2023.04.003

PAAc-NHS酯/明胶复合水凝胶口腔贴剂的性能研究

敦芷悦¹,孙川越²,张志豪¹,舒菲¹,张青红²,刘梅¹()

1.南京医科大学附属口腔医院修复科,江苏省口腔疾病研究重点实验室,江苏省口腔转化医学工程研究中心,江苏南京(210029)
2.东华大学材料科学与工程学院纤维材料改性国家重点实验室,上海(201620)

修回日期:2023-01-09 出版日期:2023-04-28 发布日期:2023-05-08
通讯作者: 刘梅 E-mail:liumei2017@njmu.edu.cn
基金资助:
国家自然科学基金青年项目(81701025);江苏省高校优势学科建设工程资助项目(2018-87);江苏省科教能力提升工程——江苏省研究型医院(YJXYYJSDW4);江苏省医学创新中心(CXZX202227)

Study on properties of PAAc-NHS ester/gelatin composite oral patches

DUN Zhiyue¹,SUN Chuanyue²,ZHANG Zhihao¹,SHU Fei¹,ZHANG Qinghong²,LIU Mei¹()

Department of Prosthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing 210029, China

Revised:2023-01-09 Online:2023-04-28 Published:2023-05-08
Contact: LIU Mei E-mail:liumei2017@njmu.edu.cn

摘要/Abstract

摘要：

目的比较不同n-羟基琥珀酰亚胺酯接枝的聚丙烯酸(PAAc-NHS酯)含量对PAAc-NHS酯/明胶复合水凝胶机械性能及体外黏附性能的影响。方法采用紫外交联法制备PAAc-NHS酯/明胶复合水凝胶,根据PAAc-NHS酯质量分数分为A(10%)、B(20%)、C(30%)3组。采用扫描电子显微镜(SEM)观察复合水凝胶表面形貌;傅里叶红外光谱(FT-IR)仪检测PAAc-NHS酯/明胶复合水凝胶表面特征性官能团;通过拉伸实验检测水凝胶机械性能;检测复合水凝胶的溶胀率与保水率;评价水凝胶的黏附性能及细胞毒性。结果 SEM结果显示PAAc-NHS酯/明胶复合水凝胶具备多孔的微观结构,孔隙大小随PAAc-NHS酯质量分数增大而减小;FT-IR结果表明各组PAAc-NHS酯均与明胶交联;拉伸结果显示C组具有最强的机械性能,拉伸应力(140.15±45.24)kPa,断裂伸长率(310.19±11.43)%;溶胀性和保水性实验结果显示A组具有最高的吸水率和保水率;体外黏附实验显示3组PAAc-NHS酯/明胶复合水凝胶均具有良好的体外黏附性能且均未表现出细胞毒性。结论各组PAAc-NHS酯/明胶复合水凝胶均具有良好的机械性能及体外黏附性能,且未显示出细胞毒性,具有作为口腔贴剂的应用潜能。

关键词: 水凝胶, 聚丙烯酸, 口腔贴剂, 伤口敷料

Abstract:

Objective To compare the effects of different levels of poly(acrylic acid)grafted with N-hydrosuccinimide ester(PAAc-NHS ester)on the mechanical properties and in vitro adhesion properties of PAAc-NHS ester/gelatin composite hydrogels. Methods The PAAc-NHS ester/gelatin composite hydrogels were constructed via ultraviolet cross-linking method and divided into three groups by mass fraction of PAAc-NHS ester:A(10%), B(20%)and C(30%). Scanning electron microscopy(SEM) was used to observe the surface morphology of the composite hydrogels, and Fourier infrared spectroscopy(FT-IR)was used to detect the surface characteristic functional groups of PAAc-NHS ester/gelatin composite hydrogels; mechanical properties of hydrogel were tested by tensile test; the swelling ratio and water retention ratio of the composite hydrogels were calculated and the adhesive properties and cytotoxicity of hydrogels were evaluated. Results SEM results showed that PAAc-NHS ester/gelatin composite hydrogel had porous microstructure, and the pore size decreased with the increase of PAAc-NHS ester content; the FT-IR results showed that PAAc-NHS ester was cross-linked with gelatin in all groups; the tensile results revealed that Group C had the strongest mechanical properties (tensile stress was (140.15±45.24) kPa; elongation at break was (310.19±11.43)%); the swelling and water retention test results showed that group A had the highest water absorption and water retention rates; in vitro adhesion experiments showed that all three groups of PAAc-NHS ester/gelatin composite hydrogels had good adhesion properties in vitro and showed no cytotoxicity. Conclusion All the groups of PAAc-NHS ester/gelatin composite hydrogels have good mechanical properties and adhesion in vitro, and have no cytotoxicity. Therefore, they have the potential to be applied as oral patches.

Key words: hydrogel, polyacrylic acid, oral patch, wound dressings

中图分类号:

R783.1

敦芷悦,孙川越,张志豪,舒菲,张青红,刘梅. PAAc-NHS酯/明胶复合水凝胶口腔贴剂的性能研究[J]. 口腔医学, 2023, 43(4): 301-307.

DUN Zhiyue,SUN Chuanyue,ZHANG Zhihao,SHU Fei,ZHANG Qinghong,LIU Mei. Study on properties of PAAc-NHS ester/gelatin composite oral patches[J]. Stomatology, 2023, 43(4): 301-307.

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组别	拉伸强度/kPa	断裂伸长率/%
A	54.54±21.39	130.93±54.09
B	73.83±22.15	217.92±75.16
C	140.15±45.24^*	310.19±11.43^*

PAAc-NHS酯/明胶复合水凝胶口腔贴剂的性能研究

Study on properties of PAAc-NHS ester/gelatin composite oral patches

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组别	丙烯酸/%	AAc-NHS酯/%	明胶/%	gelMA/%	α-酮戊二酸/%	去离子水/%
A	10	0.33	10	0.1	0.2	79.37
B	20	0.67	10	0.1	0.2	69.03
C	30	1.00	10	0.1	0.2	58.70

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