不同处理液对脱位牙牙牙周膜修复作用的实验研究

摘要/Abstract

摘要： 目的　　观察比较Hanks′平衡盐溶液(简称HBSS)、牛奶及2%NaF这几种不同处理液对干燥放置的脱位牙牙周膜作用效果的差异，为临床选择适宜的处理液提供实验依据。方法　收集因正畸治疗需要而拔除的前磨牙277颗模拟外伤脱位牙，在不同干燥放置时间点分别用HBSS、牛奶及2%NaF进行浸泡处理后，采用台盼蓝染色计数法及HE染色技术，观察比较各种条件下根面牙周膜细胞的存活状况及组织学表现。结果　干燥时间为0.5 h时，HBSS组牙周膜细胞存活率（94.60±0.72）%，大于2%NaF组（84.37±3.98）%，差异有显著性（P＜0.05）；干燥时间为2、4 h时，HBSS组牙周膜细胞存活率（63.03±3.05）%、（13.38±1.04）%大于牛奶组（48.9±3.47）%、（5.73±1.29）%及2%NaF组（51.93±1.72）%、（8.60±0.74）%，有统计学意义（P＜0.05）。结论　不同处理液对于离体干燥保存的脱位牙的牙周膜的修复作用各有不同，HBSS的效果相对较好。

关键词: 脱出牙, 牙周膜, 处理液

Abstract: Abstract: Objective To provide fundamental data for understanding the difference between various treatment fluids and to investigate the best treatment fluid for teeth replantation. Methods 277 premolars extracted for orthodontics will be collected to simulate the avulsed teeth and then soaked in HBSS, milk and 2% NaF at different drying time. Trypan blue staining technique will be used to detect the viability of PDL cells, and then subjected to statistical analysis using analysis of variance. And the morphologic changes of PDL will be observed under the microscope. Results The viability of PDL cells soaked in HBSS showed higher viability（94.60±0.72）than that in 2%NaF（84.37±3.98) at 30mins; At 60mins and 120mins, the viability of PDL cells soaked in HBSS(63.03±3.05、13.38±1.04) was higher than that in milk(48.9±3.47、5.73±1.29) and 2%NaF(51.93±1.72、8.60±0.74) respectively. Conclusions All the three solutions have repaired effect on viability of the PDL cells ,and HBSS is the best one.

Key words: avulsed teeth, periodontal ligament, treating solution

中图分类号:

R782.12

薛阳高洁. 不同处理液对脱位牙牙牙周膜修复作用的实验研究[J]. , 2015, 35(10): 813-817.

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