GPNMB对口腔鳞癌细胞增殖、侵袭影响的研究

›› 2015, Vol. 35 ›› Issue (10): 818-821.

GPNMB对口腔鳞癌细胞增殖、侵袭影响的研究

刘欣¹,谢鑫²

1. 陕西省咸阳市铁一局咸阳医院
2. 西北大学

收稿日期:2014-07-23 修回日期:2014-09-04 出版日期:2015-10-28 发布日期:2015-10-27
通讯作者: 刘欣 E-mail:huanghai598@163.com

Effect of GPNMB on proliferation and invasion of oral squamous cell carcinoma cells

Received:2014-07-23 Revised:2014-09-04 Online:2015-10-28 Published:2015-10-27

摘要/Abstract

摘要： 目的　　研究非转移性黑色素瘤糖蛋白B（glycoprotein (transmembrane) nonmetastatic melanoma protein b，GPNMB）对口腔鳞癌细胞增殖、侵袭的影响。方法　采用RT-PCR及Western blot法检测口腔鳞癌组织、口腔鳞癌细胞中GPNMB的表达变化；构建GPNMB-siRNA载体，转染细胞后分别采用MTT法、Transwell小室法观察其对Tca-8113细胞增殖、侵袭的影响。结果　GPNMB在口腔鳞癌组织及口腔鳞癌细胞中均呈现高表达；增殖实验结果表明GPNMB-siRNA可显著抑制Tca-8113增殖；细胞侵袭结果表明GPNMB-siRNA可显著抑制Tca-8113侵袭。结论　GPNMB在口腔鳞癌细胞的增殖和侵袭中起着重要作用。

关键词: 非转移性黑色素瘤糖蛋白B, 口腔鳞癌细胞, 增殖, 侵袭

Abstract: AIM: To investigate the effect of glycoprotein (transmembrane) nonmetastatic melanoma protein b (GPNMB) on proliferation and invasion of oral squamous cell carcinoma cells. METHODS: The expression levels of GPNMB in oral squamous cell carcinoma tissues and oral squamous cell carcinoma cells were determined by RT-PCR and Western blot, respectively. GPNMB-siRNA was constructed, and then transfected into Tca-8113 cells. MTT assay and transwell chamber were used to determine the effect of GPNMB-siRNA on Tca-8113 cell proliferation and invasion. RESULTS: GPNMB were up-regulated in oral squamous cell carcinoma tissues and oral squamous cell carcinoma cells. In addition, MTT assay indicated that the proliferation of Tca-8113 cells was significantly inhibited by GPNMB-siRNA, and GPNMB-siRNA also markedly inhibited Tca-8113 cell invasion. CONCLUSION: GPNMB may play an important in Tca-8113 cell proliferation and invasion, which laid the foundation for studying the mechanism of GPNMB in oral squamous cell carcinoma.

Key words: GPNMB, oral squamous cell carcinoma, proliferation, invasion

中图分类号:

R739.8

刘欣谢鑫. GPNMB对口腔鳞癌细胞增殖、侵袭影响的研究[J]. , 2015, 35(10): 818-821.

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