miRNA-448在肿瘤中作用的研究进展

›› 2015, Vol. 35 ›› Issue (10): 889-892.

miRNA-448在肿瘤中作用的研究进展

沈林汉¹,李宏卫²,詹甜甜³,谢龙²

1. 南京医科大学研究生院
2. 南京医科大学附属口腔医院
3. 南京医科大学

收稿日期:2015-02-03 修回日期:2015-04-15 出版日期:2015-10-28 发布日期:2015-10-27
通讯作者: 沈林汉 E-mail:334130939@qq.com
基金资助:
江苏省高校优势学科建设工程资助项目

The research progress of functions of microRNA-448 in the tumor

Received:2015-02-03 Revised:2015-04-15 Online:2015-10-28 Published:2015-10-27

摘要/Abstract

摘要： microRNA（miRNA）是广泛存在于真核生物中的一种具有高度保守序列的非编码蛋白，长度为20到24个核苷酸，它参与细胞增殖、分化、凋亡等多种细胞活动的调控。其中miRNA448会降低脂肪细胞的分化，与甘油三酯的堆积和脂肪细胞的表达相关，参与肥胖的自我调控机制。而且miRNA-448在研究中被发现通过影响丙型肝炎病毒的复制，来影响机体被微生物感染后的免疫反应。同时在肝癌、乳腺癌、胶质瘤等肿瘤中的表达异常，成为一种新的生物标记物，并通过与多个靶基因SATB1，NF-κB和5-HT2B等的作用参与癌症分化、侵袭、转移。近来我们在口腔鳞状细胞癌（OSCC）中通过高通量深度测序技术构建miRNA和mRNA表达谱,发现miRNA-448在OSCC中对比癌旁组织增高10倍，为数百种检测的miRNA中增高最多，miRNA-448可能在OSCC中起着十分重要的作用，但是miRNA-448在OSCC中尚无任何研究报道，为了奠定miRNA-448在OSCC中作用的深化认识，因此在此对miRNA-448的研究进展进行综述。

关键词: miRNA, miRNA-448, 口腔鳞状细胞癌, 研究进展

Abstract: MicroRNA (miRNA) are highly conservative sequence of the coding protein widely existing in eukaryotic organism .The mature miRNA are 20~24 nucleotides, which can regulate a variety of cellular activities including proliferation, differentiation, and apoptosis. miRNA-448 can regulate adipocytes differentiation; it influences the accumulation of triglycerides and expression adipocytes, and also gets involved in the self-regulatory mechanisms of obesity. Moreover, it was found in a study that miRNA-448 could impact the immune response of the organism after microorganism infection through influencing the replication of hepatitis C virus. MiRNA-448 becomes a new biomarker due to its abnormal expression in the liver cancer、breast cancer、glioma and other tumors, and miRNA-448 is involved in cancer differentiation, invasion, transfer by the action of a plurality of target genes like STAB1、κB and5-HT2B . Recently we built a expression profile of miRNA and mRNA through a high-throughput deep sequencing technology in oral squamous cell carcinoma (OSCC) and found that miRNA-448 increased 10-fold compared to adjacent tissues and became the most elevated one in the hundreds of detected miRNA, so it may play an important role in OSCC. However, there is no research reporting miRNA-448 in OSCC yet. To enhance deep understanding of the role of miRNA-448 in OSCC, we are reviewing the progress of miRNA-448.

Key words: miRNA, oral squamous cell carcinoma, research progress

中图分类号:

沈林汉李宏卫詹甜甜谢龙. miRNA-448在肿瘤中作用的研究进展[J]. , 2015, 35(10): 889-892.

参考文献

[1]Bartel DP.MicroRNAs: target recognition and regulatory functions[J].Cell,2009,136(2):215-233
[2]Siomi H,Siomi MC.Posttranscriptional regulation of microRNAbiogenesis in animals[J].Mol Cell,2010,38(3):323-332
[3]Bhayani MK,Calin GA,Lai SY.Functional relevance of miRNA sequences in human disease[J].Mutat Res,2012,731(1-2):14-19
[4]Lee RC,Feinbaum RL,Ambros V.The Celegans heterochronic genelin-4 encodes small RNAs with antisense complementarity to lin-14[J].. Cell,1993,75(5):843-854
[5]Pasquinelli AE,Reinhart BJ,Slack F,et al.Conservation of the sequence and temtral expression of let-7 heterechronic regulatory[J].Nature,2000,408(6808):86-89
[6]Kinoshita M,Ono K,Horie T,et al.Regulation ofadipocyte differentiation by activation of serotonin (5-HT)receptors 5-HT2AR and 5-HT2CR and involvement of miRNA-448-mediated repression of Klf5[J].Mol Endocrinol,2010,24(10):1978-1987
[7]侯召华,张建,田志刚.MicroRNA 调控固有免疫应答的分子机制[J].生物化学与生物物理进展.2008,35(10):1131-113
[8]Xu G,Zhang Y,Jia H,et al.Cloning and identification of microRNAs in bovine alveolar macrophages[J].Mol Cell Biochem,2009,332(1-2):9-16
[9]Pedersen IM,Cheng G,Wieland S，et al.Interferon modulation of cellular microRNAs as an antiviral mechanism[J].Nature,2007,449(7164):919-92
[10]Pedersen I,David M.MicroRNAs in the immuneresponse[J].Cytokine,2008,43(3):391-394
[11]Conrad KD,Niepmann M.The role of microRNAs in hepatitis C virus RNA replication[J].Arch Virol,2014,159(5):849-862
[12]An J,Cai T,Che H,et al.The changes of miRNA expression in rat hippocampus following chronic lead exposure[J].Toxicol Lett,2014,229(1):158-166
[13]Katayama Y,Maeda M,Miyaguchi K,et al.Identification of pathogenesis-related microRNAs in hepatocellular carcinoma by expression profiling[J].Oncol Lett,2012,4(4):817-823
[14]Huber MA,Kraut N,Beug H.Molecular requirements for epithelial-mesenchymal transitionduring tumor progression[J].Curr Opin Cell Biol,2005,17(5):1-11
[15]Li QQ,Chen ZQ,Cao XX,et al.Involvement of NF-jB/miR-448 regulatory feedback loop in chemotherapy-inducedepithelial–mesenchymal transition of breast cancer cells[J].Cell Death and Differentiation,2011,18(1):16-25
[16]刘思玉,谢龙,祁兵,马长艳,桑磊,李宏卫.口腔鳞状细胞癌组织中差异微小RNA/mRNA表达谱的对接研究[J].华西口腔医杂志,2014,32(4):400-403