›› 2015, Vol. 35 ›› Issue (11): 897-900.

• 基础研究 •    下一篇

隔山消不同溶剂提取物对脱矿釉质再矿化作用的实验研究

王丽梅1,刘字珍2,吕再玲2,蓝海3   

  1. 1. 1大理州医院口腔科,云南大理(671000);2.大理大学药学与化学学院,云南大理 (671000)
    2. 大理大学药化学院
    3. 云南省大理大学
  • 收稿日期:2015-05-12 修回日期:2015-07-14 出版日期:2015-11-28 发布日期:2015-11-28
  • 通讯作者: 蓝海 E-mail:lanhai8696@126.com
  • 基金资助:
    国家自然科学基金;大理学院应用开发研究基金

Experimental Study on remineralization of demineralized enamel by different extracts of Geranium Strictipes R.Kunth

  • Received:2015-05-12 Revised:2015-07-14 Online:2015-11-28 Published:2015-11-28

摘要: [摘要] 目的 通过测试隔山消不同溶剂提取物处理人工龋齿后的显微硬度,并用SEM观察其表面形态,研究隔山消不同溶剂提取物对人工脱矿釉质再矿化的影响。方法 用化学方法建立人工脱矿釉质龋模型,以乙酸乙酯提取物,正丁醇提取物,水层提取物为实验溶液,20 mg/L的氟化钠为阳性对照,去离子水为阴性对照将其分为5组,经体外pH循环后,测试其显微硬度值,SEM观察表面形态。结果 显微硬度显示隔山消3种不同溶剂提取物和NaF经再矿化处理后,各组釉质硬度较再矿化前明显增高(P<0.05),去离子水组再矿化前后无明显差异(P>0.05),各组组间比较有统计学意义(P<0.05 )。说明隔山消不同溶剂提取物和NaF对脱矿牛切牙有再矿化作用。SEM显示,氟化钠组有紧密均匀的沉淀区,去离子水组表面呈疏松结构,正丁醇组有紧密的矿物沉积,并有很小的空隙,乙酸乙酯组矿物沉淀较少,并有较大空隙,水层组有规则的颗粒沉积物生成。 结论 隔山消不同溶剂提取物对人工脱矿釉质均有再矿化作用,其作用从强到弱为:水层提取部位,正丁醇提取部位,乙酸乙酯提取部位。

关键词: 隔山消, 釉质, 再矿化, 显微硬度, 扫描电镜

Abstract: Abstract: Objective To test microhardness of artificial caries which was treated by different extracts of Geranium Strictipes R.Kunth,to observe its surface morphology by SEM,and to study the effects of different solvent extracts on remineralization of artificial demineralized enamel.Methods 5 groups of artificial demineralized enamel caries models were established by chemical methods:the group of ethyl acetate extract,the group of butanol extract,the group of water layer extract,20 mg/mL NaF positive control group,and deionized water negative control group.After in vitro pH cycling,microhardness was tested and the surface morphology was observed by SEM.Results Microhardness results showed that after the remineralization treatment by three different extracts and NaF,the enamel hardness in each group significantly increased than that before remineralization (P<0.05),while the deionized water group showed no significant remineralization difference (P>0.05),and comparison between groups was statistically significant (P<0.05),denoting the remineralization effects of different extracts of Geranium Strictipes R.Kunth and NaF on demineralized bovine incisors.SEM results showed there was uniform and close precipitation zone in NaF group,loose surface structure in deionized water group,close mineral deposits and a small gap in the group of butanol extract,less mineral deposits and a larger gap in the group of ethyl acetate extract,and regular particle deposits in the group of water layer extract.Conclusions The different extracts of Geranium Strictipes R.Kunth have remineralization effects on artificial demineralized enamel.The best effect was obtained by water layer extract,then butanol extract,and lastly ethyl acetate extract.

Key words: Geranium Strictipes R.Kunth, enamel, remineralization, microhardness, SEM

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