去甲肾上腺素对FaDu鳞癌细胞系生物学行为的影响

›› 2016, Vol. 36 ›› Issue (1): 6-11.

去甲肾上腺素对FaDu鳞癌细胞系生物学行为的影响

马超,孙沫逸,倪前伟,柴娟,申志远,杨向明,鞠骏,高涛,李昀,赵振彦

第四军医大学口腔医学院

收稿日期:2015-07-28 修回日期:2015-09-20 出版日期:2016-01-28 发布日期:2016-01-08
通讯作者: 孙沫逸 E-mail:moyisun@163.com
基金资助:
p12CDK2-AP1与新发现的结合蛋白Nbp相互作用的相关研究。;涎腺腺样囊性癌嗜神经侵袭模型的建立及相关研究

Effects of norepinephrine on the biological behavior of human pharyngeal squamous cell carcinoma cell line FaDu

Received:2015-07-28 Revised:2015-09-20 Online:2016-01-28 Published:2016-01-08

摘要/Abstract

摘要： 目的　研究去甲肾上腺素对人咽鳞状细胞癌细胞系FaDu增殖、侵袭及迁移等细胞生物学行为的影响。方法　分别采用MTT实验、Transwell细胞侵袭及迁移实验和划痕实验检测不同浓度去甲肾上腺素对人咽鳞状细胞癌细胞系FaDu增殖、侵袭及迁移能力的影响，同时观察普萘洛尔预处理对去甲肾上腺素作用的干预效果。结果　MTT实验、Transwell实验和划痕实验结果表明浓度为10 μmo/L的去甲肾上腺素可以显著促进人咽鳞状细胞癌细胞系FaDu的增殖、侵袭及迁移（P＜0.001），而浓度为1 μmol/L的普萘洛尔预处理可以有效抑制去甲肾上腺素对人咽鳞状细胞癌细胞系FaDu增殖、侵袭及迁移的促进作用（P＜0.001）。结论　去甲肾上腺素对人咽鳞状细胞癌细胞系FaDu的增殖、侵袭及迁移有促进作用，而非选择性β肾上腺素能受体阻断剂普萘洛尔可以抑制该促进作用。

关键词: 人咽鳞状细胞癌细胞系FaDu, 去甲肾上腺素, 普萘洛尔, 增殖, 侵袭, 迁移

Abstract: Objective To investigate the effects of norepinephrine(NE) on thebiological behavior of human pharyngeal squamous cell carcinoma cell line FaDu.MethodsMTT assay was used to evaluate the influence of NEontheproliferation of tumor cells. Transwellinvasion assay was used to evaluate theinfluence of NE on theinvasion of tumor cells.The effects of NE on themigrationof tumor cells wasmeasuredby Transwellmigration assayand wound-healing assay.Additionally, theeffects of the β-adrenergic antagonist propranolol on the NE-induced proliferation, invasion and migrationof tumor cellswere measured.Results10μmol/LNE promotedtheproliferation, invasion and migration of tumor cells significantly(P<0.001). However,pretreatment with 1μmol/L propranolol effectivelyinhibitedthe NE-induced proliferation, invasion and migration(P<0.001). ConclusionNEpromotestheproliferation, invasion and migration of human pharyngeal squamous cell carcinoma cells.These effectscan be suppressed by propranolol.

Key words: human pharyngeal squamous cell carcinoma cell line FaDu, norepinephrine, propranolol, proliferation, invasion, migration

中图分类号:

R739.8

马超孙沫逸倪前伟柴娟申志远杨向明鞠骏高涛李昀赵振彦. 去甲肾上腺素对FaDu鳞癌细胞系生物学行为的影响[J]. , 2016, 36(1): 6-11.

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