Nestin阳性细胞过表达DMP1对小鼠颌骨密度的影响

›› 2016, Vol. 36 ›› Issue (10): 870-875.

Nestin阳性细胞过表达DMP1对小鼠颌骨密度的影响

潘敏¹,孙瑶²

1. 同济大学附属口腔医院
2. 同济大学口腔医学院

收稿日期:2016-05-30 修回日期:2016-07-05 出版日期:2016-10-28 发布日期:2016-10-25
通讯作者: 孙瑶 E-mail:Sunyao919@126.com
基金资助:
国家自然基金;国家自然基金;2013年教育部博士点基金;上海市卫生系统新优青项目

Influence of over expression of DMP1 in Nestin positive cells on the changes of jaw bone density in mice

PAN Min,

Received:2016-05-30 Revised:2016-07-05 Online:2016-10-28 Published:2016-10-25

摘要/Abstract

摘要： 目的探讨Nestin阳性细胞中牙本质基质蛋白1（DMP1)的作用。方法 DMP1是牙齿和骨中重要的矿化蛋白，但是Nestin阳性细胞中DMP1的作用仍不清楚。我们构建了Nestin 阳性细胞中过表达DMP1的转基因小鼠。以野生型（wild type,WT)小鼠为对照组，通过H&E染色，Micro CT检测DMP1-Tg小鼠颌骨的变化，进一步通过TRAP染色检测DMP1-Tg小鼠颌骨破骨方面的变化。结果相对于WT小鼠， DMP1-Tg小鼠颌骨骨量下降；TRAP染色显示DMP1-Tg小鼠下颌骨破骨细胞增多。结论在下颌骨发育过程中，Nestin阳性细胞中过表达DMP1对骨形成发挥抑制作用。

关键词: Nestin, Dental matrix protein 1, 颌骨

Abstract: Objective To discuss the role of dental matrix protein 1（DMP1） in Nestin(+) cells. Methods DMP1 is an important mineralization protein of teeth and bones, but the role of DMP1 in Nestin(+) cells is still not clear. A transgenic mouse model was constructed in which DMP1 over expressed in Nestin(+) cells. With wild mice as the control group, the DMP1-Tg mouse’s jaw phenotypes by H&E staining and micro-CT were observed. Besides, the change of bone resorption in DMP1-Tg mouse by TRAP staining was also observed. Results Compared with WT mouse, the mandibular bone mass of DMP1-Tg mouse decreased; TRAP staining showed increased number of osteoclasts in DMP1-Tg mouse. Conclusion During the process of jaw development, the over expression of DMP1 in Nestin(+) cell can inhibit osteogenesis.

Key words: Nestin, Dental matrix protein 1, Jaw bone

中图分类号:

R329.24

潘敏孙瑶. Nestin阳性细胞过表达DMP1对小鼠颌骨密度的影响[J]. , 2016, 36(10): 870-875.

PAN Min. Influence of over expression of DMP1 in Nestin positive cells on the changes of jaw bone density in mice [J]. , 2016, 36(10): 870-875.

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