TNF-α刺激髁突软骨细胞产生白细胞介素-34的研究

›› 2016, Vol. 36 ›› Issue (11): 968-971.

TNF-α刺激髁突软骨细胞产生白细胞介素-34的研究

吴立立¹,史秋涛²,徐高丽²,谷志远²

1. 浙江省中医药大学
2. 浙江中医药大学

收稿日期:2016-06-03 修回日期:2016-07-16 出版日期:2016-11-28 发布日期:2016-11-29
通讯作者: 谷志远 E-mail:gzy@zju.edu.cn
基金资助:
国家自然科学基金

A study of the production of interleukin-34 in condylar cartilage cells stimulated by TNF-α

Received:2016-06-03 Revised:2016-07-16 Online:2016-11-28 Published:2016-11-29

摘要/Abstract

摘要： 目的通过观察TNFα对髁突软骨细胞分泌白细胞介素-34（IL34）及表达IL-34 mRNA基因的影响，探讨IL34在颞下颌关节紊乱病中的致病机制。方法取第三代髁突软骨细胞，培养过程于培养液中加入不同浓度（0、1、10、20、50 ng/mL）的TNFα培养24 h以及同一浓度（10 ng/mL）TNFα分别培养1、3、6、10、24 h后，收集培养上清液及细胞，分别用ELISA和RTPCR方法测定IL-34浓度以及IL34 mRNA的表达情况。结果 ①实验组和对照组髁突软骨细胞上清液中均能检测到IL-34。②TNFα刺激髁突软骨细胞IL-34mRNA基因表达，并呈正相关关系。结论 TNF-α能刺激髁突软骨细胞产生IL34。

关键词: 白细胞介素-34, 髁突软骨细胞, 肿瘤坏死因子-α, 大鼠

Abstract: Objective To address the expression of IL-34 regulated by TNF-α in SD rats mandibular condylar cartilage (MCC) cells and to investigate its regulation and pathogenic role in temporomandibular disorders. Methods The third generation of condylar cartilage cells were taken. A variety of concentrations (0, 1, 10, 20, 50 ng/mL) of TNFα were added to the culture medium for 24h and cells were cultured at increasing timepoints (1, 3, 6, 10, 24 h) in the presence of TNF-α (10 ng/mL). The production of IL34 was measured using enzymelinked immunosorbent assay (ELISA)and IL-34 mRNA levels were determined by realtime reverse transcriptionpolymerase chain reaction (RTPCR). Results ①IL-34 was detected in MCC cells; ②The production of IL-34mRNA was enhanced by TNF-α in a timedependent and dosedependent manner. Conclusion This data provides novel information about the production of IL-34 stimulated by TNF-α in MCC cells.

Key words: interleukin-34, condylar cartilage cells, tumor necrosis factor-α, rat

中图分类号:

R782.6

吴立立史秋涛徐高丽谷志远. TNF-α刺激髁突软骨细胞产生白细胞介素-34的研究[J]. , 2016, 36(11): 968-971.

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