多样本人腮腺间充质干细胞诱导iPS细胞的对比研究

›› 2016, Vol. 36 ›› Issue (11): 972-975.

多样本人腮腺间充质干细胞诱导iPS细胞的对比研究

颜兴

首都医科大学附属北京友谊医院

收稿日期:2016-06-13 修回日期:2016-07-13 出版日期:2016-11-28 发布日期:2016-11-29
通讯作者: 颜兴 E-mail:happytooth@yeah.net
基金资助:
国家自然科学基金;北京市卫生系统高层次卫生技术人才培养计划项目

Comparative study on the induction of iPS cells by multisample mesenchymal stem cells from human parotid gland

Xing Yan

Beijing friendship hospital，capital medical university

Received:2016-06-13 Revised:2016-07-13 Online:2016-11-28 Published:2016-11-29
Contact: Xing Yan E-mail:happytooth@yeah.net

摘要/Abstract

摘要： 目的研究比较不同样本腮腺间充质干细胞（hPMSCs）生成诱导多潜能干细胞（iPSCs）的效果。方法分别分离培养3个患者的hPMSCs，应用游离质粒混合因子（OCT3/4、SOX2、KLF4、cMYC、LIN28、TP53 shRNA）电转导法重编程诱导获得iPS细胞（hPMSCiPSCs）。比较在相同条件下3个不同供体来源腮腺细胞诱导iPS细胞的差异。结果 3个不同样本人腮腺间充质干细胞都成功诱导iPSCs，形成率分别为0.11%、0.10%、0.09%，无显著性差异（P＞0.05）；诱导形成iPS克隆时间有差异（P<0.05）；形成iPSCs后维持培养倍增时间无差异（P＞0.05）。结论不同患者可分离获得hPMSCs后成功诱导hPMSCiPSCs，诱导效率无差异，诱导时间有差异。

关键词: 诱导多潜能干（iPS）细胞, 腮腺, 间充质干细胞

Abstract: Objective To study and compare the effects of the generation of induced pluripotent stem cells (iPSCs) from different samples of parotid mesenchymal stem cells (hPMSCs). Methods The hPMSCs of three patients were isolated and cultured, then were used by a combination of sufcient episomal reprogramming vectors that consist of OCT3/4, SOX2, KLF4, LMYC, LIN28 and shRNA targeting TP53 to generate iPSCs. The difference of iPS cells induced among three sources of parotid cells on the same condition was compared. Results Three samples of parotid gland mesenchymal stem cells were successful in inducing iPSCs, and their formation rates were respectively 0.11%, 0.10% and 0.09%, no significant difference (P＞ 0.05); formation time of iPSC clones induced was different (P< 0.05); the doubling time of hPMSCiPSCs was no difference in maintaining culture after iPSCs formation (P＞ 0.05). Conclusion The hPMSCiPSCs can be successfully induced by hPMSCs from different patients, and there is no difference in induction efficiency, while exits the difference in induction time.

Key words: induced pluripotent stem (iPS) cell, parotid gland, mesenchymal stem cell

中图分类号:

R329.28

颜兴. 多样本人腮腺间充质干细胞诱导iPS细胞的对比研究[J]. , 2016, 36(11): 972-975.

Xing Yan. Comparative study on the induction of iPS cells by multisample mesenchymal stem cells from human parotid gland[J]. , 2016, 36(11): 972-975.

参考文献

参考文献
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