茶黄素预防龋病的动物实验

›› 2016, Vol. 36 ›› Issue (11): 976-979.

茶黄素预防龋病的动物实验

高佳雨¹,王伊娜¹,庞景威¹,傅露¹,黎红²

1. 浙江中医药大学
2. 杭州口腔医院

收稿日期:2016-04-15 修回日期:2016-08-19 出版日期:2016-11-28 发布日期:2016-11-29
通讯作者: 黎红 E-mail:lihong8689@163.com
基金资助:
国家自然科学基金;杭州市重点专科专病科研攻关专项

Effects of TFs on caries’ development in rats

Received:2016-04-15 Revised:2016-08-19 Online:2016-11-28 Published:2016-11-29

摘要/Abstract

摘要： 目的通过动物实验探讨茶黄素（Theaflavins，TFs）对龋病的预防作用。方法选择SD大鼠24只，随机分成TFs组、NaF组、蒸馏水组(对照组)，每组8只，雌雄各半。接种变形链球菌（S.mutans）且给予致龋饲料2000#和5%蔗糖水，分别用浓度为最小抑菌浓度（minimum inhibitory concentration，MIC）的TFs、250 mg/L NaF溶液及蒸馏水冲洗。连续处理5周后处死大鼠，Keyes记分法观察，评估大鼠磨牙患龋情况。实验数据用SPSS 22.0统计软件进行分析。结果经龋齿Keyes记分发现TFs对大鼠龋齿产生不同程度抑制，使光滑面牙本质龋浅龋和窝沟龋牙本质中龋患病率分别下降85.0%、62.5%。结论 MIC浓度的TFs对大鼠牙本质龋损具有明显的抑制作用。

关键词: 龋病, 茶黄素, 动物模型, 变形链球菌

Abstract: Objective Through the animal experiment to observe the effectiveness of TFs on the prevention of the dental caries. Methods Twentyfour 23-dayold SD rats were divided randomly into three groups (TFs、NaF、H2O), 8 rats per group, half male and half female. All the rats were inoculated with S.mutans and fed with cariesinducing diet 2000 and the 5% sugar water, and then rats’ were rinsed with the MIC purity TFs、250 mg/L NaF solution and distilled water separately. After 5 weeks’ sequential processing execution, rats were sacrificed for scoring caries molar teeth by Keyes' method, and the rats’ molar caries situation was evaluated. Finally, the data collected were analyzed with SPSS 22.0 version. Results The Keyes scores showed rats under TFs appeared different degrees of caries’ lesion, and it had reduced the prevalence rates of dental smooth surface caries by 85.0% and sulcal surface dentine caries by 62.5%. Conclusions The TFs in MIC has an obvious inhibitory effect on the dentine caries of rats.

Key words: dental caries, Theaflavins, model animal, S.mutans

中图分类号:

R781.1

高佳雨王伊娜庞景威傅露黎红. 茶黄素预防龋病的动物实验[J]. , 2016, 36(11): 976-979.

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