低氧诱导因子-2α在不同应力加载髁突软骨细胞中的表达及意义

›› 2016, Vol. 36 ›› Issue (2): 101-104.

低氧诱导因子-2α在不同应力加载髁突软骨细胞中的表达及意义

李文¹,丁王辉¹,谈龙²,施洁珺¹

1. 浙江大学医学院附属口腔医院
2. 浙江大学附属口腔医学院

收稿日期:2015-08-28 修回日期:2015-11-08 出版日期:2016-02-28 发布日期:2016-02-29
通讯作者: 施洁珺 E-mail:sjiejun2013@163.com
基金资助:
浙江省自然科学基金项目;浙江省科技厅公益技术应用研究项目

The expression and significance of HIF2α in condyle chondrocytes under different stress loadings

Received:2015-08-28 Revised:2015-11-08 Online:2016-02-28 Published:2016-02-29

摘要/Abstract

摘要： ［摘要］目的研究低氧诱导因子-2α（HIF-2α）及其下游分解代谢因子（MMP3，MMP13，ADAMTs-4）在不同应力加载髁突软骨细胞中的表达变化情况。方法对培养到第3代的新生SD大鼠髁突软骨细胞采用5%的低氧培养箱培养。0.5 Hz下分别加载0，1 000，2 000，4 000单位压力，2 h后立即收集样本，台盼蓝染色，光镜下观察细胞形态并计数细胞数，计算死细胞率。采用westernblot及RTPCR技术检测HIF-2α及其下游分解代谢因子（MMP3， MMP13，ADAMTs-4）的蛋白及基因表达。结果随着压力的增加，髁突软骨细胞死亡率显著上升（P＜0.05）。HIF-2α及其下游分解代谢因子（MMP3， MMP13，ADAMTs-4）的表达相应增加（P＜0.05）。结论超负荷压力激活HIF-2α，继而激活下游分解代谢因子可能是导致髁突软骨损伤的一个机制。

关键词: 低氧诱导因子-2α, 分解代谢因子, 低氧培养, 应力加载, 髁突软骨

Abstract: Abstract: Objective To explore the expression of HIF-2α and its downstream catabolic factors （MMP3， MMP13， ADAMTs-4） in the mandibular condyle chondrocytes （MCC） under different stress loadings. Methods The third generation of MCC were cultured in 5% hypoxia. The uniaxial pressure of 0，1000，2000，4000 units strain （ust） were exerted on the MCC for 2 h. Then the specimens were collected and dyed by trypan blue staining. The morphology and number of cells were observed under a light microscope， and the cell mortality was calculated. Westernblot and RTPCR were performed to detect the changes of expression of HIF-2α and its downstream catabolic factors （MMP3， MMP13， ADAMTs-4）. Results With the increase of pressure， the cell mortality increased （P＜0.05）. The expression of HIF-2α and its downstream catabolic factors （MMP3， MMP13， ADAMTs-4） increased with the increase of pressure （P＜0.05）. Conclusion The activation of HIF-2α and its downstream catabolic factors by overload pressure may be responsible for the damage of condyle chondrocytes.

Key words: HIF-2α, Catabolic factor, Hypoxia, Stress loading, Condyle chondrocyte

中图分类号:

R782.6

李文丁王辉谈龙施洁珺. 低氧诱导因子-2α在不同应力加载髁突软骨细胞中的表达及意义[J]. , 2016, 36(2): 101-104.

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