Nd:YAG激光对口腔鳞癌CAL-27细胞迁移的影响

›› 2016, Vol. 36 ›› Issue (2): 109-111.

Nd:YAG激光对口腔鳞癌CAL-27细胞迁移的影响

徐诗语¹,吴国英²,李留炀³,尚进³,钱棱³,陶楠楠³,王培³,仇嘉颖³

1. 南京医科大学附属口腔医院牙体牙髓粘膜科
2. 南京医科大学附属口腔医院-江苏省口腔医院
3. 南京医科大学

收稿日期:2015-10-21 修回日期:2015-11-26 出版日期:2016-02-28 发布日期:2016-02-29
通讯作者: 吴国英 E-mail:wuguoying888@163.com
基金资助:
大学生实践创新训练计划基金

The effect of 1064nm Nd:YAG laser irradiation on the migration of oral squamous cell carcinoma CAL-27 cells

Received:2015-10-21 Revised:2015-11-26 Online:2016-02-28 Published:2016-02-29
Contact: wu guoYING E-mail:wuguoying888@163.com

摘要/Abstract

摘要： ［摘要］目的探讨1064 nm Nd:YAG激光对口腔鳞癌CAL-27细胞迁移的影响。方法体外培养鳞癌CAL-27细胞，取处于对数生长期的细胞接种于24孔板中并继续培养24 h，分0、120、240、360、480 s五组接受激光照射，对应剂量为0、44.4、88.8、133.2、177.6 J/cm，划痕后分别于0、12、24、48 h在倒置显微镜下观察划痕变化并拍照，以研究激光照射对细胞迁移影响。结果低剂量激光照射后细胞划痕区域愈合程度提高，而高剂量激光照射后，细胞划痕区域愈合程度降低。结论低剂量Nd:YAG激光照射对鳞癌CAL-27细胞迁移有一定促进作用，而高剂量Nd:YAG激光照射则会对鳞癌CAL-27细胞的迁移产生明显抑制作用。

关键词: Nd：YAG激光, 鳞癌细胞, 细胞迁移

Abstract: Abstract: Objective To investigate the effect of 1064nm Nd:YAG laser irradiation on migration of oral squamous cell carcinoma CAL-27 cells. Methods Squamous carcinoma CAL-27 cells were cultured in vitro, and cells in logarithmic growth phase were inoculated in 24-well plates and irradiated with 1064nm Nd:YAG continuous wave laser for different time intervals (0, 120, 240, 360, 480 s) with different corresponding doses (0, 44.4, 88.8, 133.2, 177.6 J/cm2). After the specimens were scratched, the healing change of those scratches were observed under inverted microscope at 0, 12, 24, 48 h. Results After a low dose of laser irradiation, the wound healing degree were improved, while after a high dose of laser irradiation, the degree of wound healing were reduced. Conclusions The squamous carcinoma CAL-27 cell migration can be stimulated by 1064nm Nd:YAG laser at a low intensity, while it can be inhibited by high intensity laser.

Key words: Nd：YAG laser, squamous carcinoma, cell migration

中图分类号:

R781.5

徐诗语吴国英李留炀尚进钱棱陶楠楠王培仇嘉颖. Nd:YAG激光对口腔鳞癌CAL-27细胞迁移的影响[J]. , 2016, 36(2): 109-111.

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