基质金属蛋白酶-2在人慢性牙周炎肥大细胞上表达的研究

摘要/Abstract

摘要： [摘要] 目的：观察基质金属蛋白酶-2（matrix metalloproteinase-2, MMP-2）在慢性牙周炎牙龈组织中肥大细胞上的表达，探讨MMP-2-tryptase双阳性肥大细胞（mast cells, MCs）在慢性牙周炎发病机制中的作用。方法：将45例参试者依据慢性牙周炎的病变程度分成3组：健康对照组15例；轻度牙周炎组15例；重度牙周炎组15例。牙龈标本经10%福尔马林液固定48 h；制作牙龈组织连续切片，HE染色，光学显微镜下观察组织学改变；采用免疫荧光双染色法，荧光显微镜下观察牙龈组织中MMP-2-tryptase双阳性MCs的表达情况。结果：与健康对照组相比，轻度和重度慢性牙周炎组牙龈组织MMP-2-tryptase双阳性MCs密度均显著升高（P<0.01）；重度慢性牙周炎组牙龈组织MMP-2-tryptase双阳性MCs密度显著高于轻度牙周炎组（P<0.05）。结论：慢性牙周炎牙龈组织MMP-2-tryptase双阳性MCs密度与牙周炎病变程度的趋势相一致，MMP-2-tryptase双阳性MCs在牙周炎的进展中可能有促进作用。

关键词: 慢性牙周炎, 肥大细胞, Tryptase, 基质金属蛋白酶-2

Abstract: Abstract Objective To investigate the expression of the matrix metalloproteinase-2 (MMP-2)-tryptase double positive mast cells (MCs) in periodontal tissues in different stages of chronic periodontitis using double immunofluorescence staining. Methods A total of 45 patients including healthy controls (n = 15), those with slight chronic periodontitis (n = 15), and those with severe chronic periodontitis (n = 15) were involved. The gingival specimens were fixed in 10% buffered formalin for 48h, stained with hematoxylin and eosin for the observation of histological changes under optical microscope, and stained with double immunofluorescence for the identification of MMP-2-tryptase double positive MCs in gingival tissues under fluorescence microscope. Results Compared with the healthy controls, the densities (cells/mm2) of MMP-2-tryptase double positive MCs were significantly increased in chronic periodontitis groups (P <0.01). In addition, the density of MMP-2-tryptase double positive MCs in severe chronic periodontitis group was significantly higher than that of the slight chronic periodontitis group (P <0.05). Conclusion The density of MMP-2-tryptase double positive MCs is positively correlated with the severity of periodontitis inflammation. MMP-2-tryptase double positive MCs may play a positive role in the development of periodontitis.

Key words: Chronic periodontitis, Mast cell, Tryptase, Matrix metalloproteinase-2

中图分类号:

R781.4

顾思成李娟黄世光. 基质金属蛋白酶-2在人慢性牙周炎肥大细胞上表达的研究[J]. , 2016, 36(5): 411-415.

参考文献

[1] Feng Z, Weinberg A. Role of bacteria in health and disease of periodontal tissues[J]. Periodontol 2000, 2006,40(1):50-76.
[2] Deo V, Bhongade ML. Pathogenesis of periodontitis: role of cytokines in host response[J]. Dent Today, 2010,29(9):60-2, 64-6; quiz 68-9.
[3] Galli SJ. Mast cells and basophils[J]. Curr Opin Hematol, 2000,7(1):32-9.
[4] Shelburne CP, Abraham SN. The mast cell in innate and adaptive immunity[J]. Adv Exp Med Biol, 2011,716:162-85.
[5] Steinsvoll S, Helgeland K, Schenck K. Mast cells--a role in periodontal diseases?[J]. J Clin Periodontol, 2004,31(6):413-9.
[6] Abraham D, Ponticos M, Nagase H. Connective tissue remodeling: cross-talk between endothelins and matrix metalloproteinases[J]. Curr Vasc Pharmacol, 2005,3(4):369-79.
[7] Birkedal-Hansen H, Moore WG, Bodden MK, et al. Matrix metalloproteinases: a review[J]. Crit Rev Oral Biol Med, 1993,4(2):197-250.
[8] Parsons SL, Watson SA, Brown PD, et al. Matrix metalloproteinases[J]. Br J Surg, 1997,84(2):160-6.
[9] Tervahartiala T, Pirila E, Ceponis A, et al. The in vivo expression of the collagenolytic matrix metalloproteinases (MMP-2, -8, -13, and -14) and matrilysin (MMP-7) in adult and localized juvenile periodontitis[J]. J Dent Res, 2000,79(12):1969-77.
[10] Naesse EP, Schreurs O, Helgeland K, et al. Matrix metalloproteinases and their inhibitors in gingival mast cells in persons with and without human immunodeficiency virus infection[J]. J Periodontal Res, 2003,38(6):575-82.
[11] Armitage GC. Development of a classification system for periodontal diseases and conditions[J]. Ann Periodontol, 1999,4(1):1-6.
[12] Buduneli N, Buduneli E, Cinar S, et al. Immunohistochemical evaluation of Ki-67 expression and apoptosis in cyclosporin A-induced gingival overgrowth[J]. J Periodontol, 2007,78(2):282-9.
[13] Steinsvoll S, Halstensen TS, Schenck K. Extensive expression of TGF-beta1 in chronically-inflamed periodontal tissue[J]. J Clin Periodontol, 1999,26(6):366-73.
[14] Batista AC, Rodini CO, Lara VS. Quantification of mast cells in different stages of human periodontal disease[J]. Oral Dis, 2005,11(4):249-54.
[15] Huang S, Lu F, Chen Y, et al. Mast cell degranulation in human periodontitis[J]. J Periodontol, 2013,84(2):248-55.
[16] 潘倩茹, 吕芳丽, 黄博, 等. 肥大细胞脱颗粒在牙周炎病理中的作用[J]. 实用口腔医学杂志, 2012,28(3):316-319.
[17] 李娟, 尹小萍, 蓝田, 等. Tryptase和TIM-1双阳性肥大细胞在不同程度人牙周炎组织中的量化研究[J]. 中国病理生理杂志, 2014,30(5):892-896.
[18] Botero JE, Contreras A, Parra B. Effects of cytomegalovirus infection on the mRNA expression of collagens and matrix metalloproteinases in gingival fibroblasts[J]. J Periodontal Res, 2008,43(6):649-57.
[19] Rai B, Kaur J, Catalina M. Bone mineral density, bone mineral content, gingival crevicular fluid (matrix metalloproteinases, cathepsin K, osteocalcin), and salivary and serum osteocalcin levels in human mandible and alveolar bone under conditions of simulated microgravity[J]. J Oral Sci, 2010,52(3):385-90.
[20] Bildt MM, Bloemen M, Kuijpers-Jagtman AM, et al. Matrix metalloproteinases and tissue inhibitors of metalloproteinases in gingival crevicular fluid during orthodontic tooth movement[J]. Eur J Orthod, 2009,31(5):529-35.
[21] Bachmeier BE, Iancu CM, Jochum M, et al. Matrix metalloproteinases in cancer: comparison of known and novel aspects of their inhibition as a therapeutic approach[J]. Expert Rev Anticancer Ther, 2005,5(1):149-63.
[22] Nagase H. Activation mechanisms of matrix metalloproteinases[J]. Biol Chem, 1997,378(3-4):151-60.
[23] Capelli J Jr, Kantarci A, Haffajee A, et al. Matrix metalloproteinases and chemokines in the gingival crevicular fluid during orthodontic tooth movement[J]. Eur J Orthod, 2011,33(6):705-11.
[24] Fang KC, Wolters PJ, Steinhoff M, et al. Mast cell expression of gelatinases A and B is regulated by kit ligand and TGF-beta[J]. J Immunol, 1999,162(9):5528-35.
[25] Johnson JL, Jackson CL, Angelini GD, et al. Activation of matrix-degrading metalloproteinases by mast cell proteases in atherosclerotic plaques[J]. Arterioscler Thromb Vasc Biol, 1998,18(11):1707-15.