纳米颗粒的细胞摄取机制及其影响因素的研究

摘要/Abstract

摘要： 由于纳米材料具有独特的性能优势，近年来纳米颗粒在临床诊断、药物载体、基因转染、组织工程、细胞示踪等方面被广泛地应用。研究纳米颗粒穿透细胞膜进入细胞内的过程以及相关的摄取机制，将有助于了解纳米材料对机体和器官的影响和作用机理，评价其对生物体潜在的危害性，这为纳米材料更安全有效地应用于生物医学领域提供了重要依据。本文将对细胞摄取纳米颗粒有关方面的近期研究做一综述。

关键词: 纳米颗粒, 摄取机制, 影响因素, 研究方法

Abstract: Due to their special properties, nanoparticles have been widely used in clinical diagnosis, drug delievery, gene transfection, tissue engineering and cell tracking. A detailed understanding of the process and mechanisms underlying the uptake of nanoparticles is important for exploring the effects and action mechanisms of nanomaterials on the biological systems and evaluating their potential harm to organisms, which will provide insight about their biological safety. This article reviewed the most recent development in the celluar uptake of nanoparticles.

Key words: Nanoparticles, Uptake mechanism, Influencing factors, Research methods

中图分类号:

R783.1

施星星周凯王琛. 纳米颗粒的细胞摄取机制及其影响因素的研究[J]. , 2016, 36(6): 573-576.

参考文献

1.Bleeker EA, de Jong WH, Geertsma RE,et al.Considerations on the EU definition of a nanomaterial: science to support policy making.Regul Toxicol Pharmacol,2013,65(1):119-25.
2.Bolocan A,Mihaiescu DE, Mesterca AR,et al.In vitro and in vivo applications of 3D dendritic gold nanostructures[J].Rom J Morphol Embryol,2015,56(3): 915-24.
3.Pinkernelle J, Raffa V, Calatayud MP,et al.Growth factor choice is critical for successful functionalization of nanoparticles[J].Front Neurosci,2015,9: 305.
4.Wu X, Lin Q, Chen G,et al.Sentinel Lymph Node Detection Using Carbon Nanoparticles in Patients with Early Breast Cancer[J].PloS one,2015,10(8): e0135714.
5.Tautzenberger A,?Kovtun A,?Ignatius A.Nanoparticles?and their potential
for?application?in?bone[J].Int J Nanomedicine,2012,7(1):4545-57.
6.Feng X, Chen A, Zhang Y,et al.Central nervous system toxicity of metallic nanoparticles[J]. Int J Nanomedicine,2015,10: 4321-40.
7.Lesniak A, Salvati A,Santos-Martinez MJ,et al.Nanoparticle adhesion to the cell membrane and its effect on nanoparticle uptake efficiency[J].J Am Chem Soc,2013,135(4):1438-44.
8.Zhang H, Burnum KE, Luna ML,et al. Quantitative proteomics analysis of adsorbed plasma proteins classifies nanoparticles with different surface properties and size[J].Proteomics,2011,11(23):4569-77.
9.Deng ZJ, Liang M, Toth I,et al.Molecular interaction of poly(acrylic acid) gold nanoparticles with human fibrinogen[J].ACS nano,2012,6(10):8962-9.
10.Fleischer CC,Payne CK.Nanoparticle surface charge mediates the cellular receptors used by protein-nanoparticle complexes[J].J Phys Chem B,2012,116 (30): 8901-7.
11.Deng ZJ, Mortimer G, Schiller T,et al.Differential plasma protein binding to metal oxide nanoparticles[J].Nanotechnology,2009,20(45):455101.
12.Kumari S, Mg S,Mayor S.Endocytosis unplugged: multiple ways to enter the cell[J].Cell Res,2010,20(3):256-75.
13.Owens DE, Peppas NA.Opsonization, biodistribution, and pharmacokinetics of polymeric nanoparticles[J].Int J Pharm,2006,307(1):93-102.
14.Lunov O, Syrovets T,Loos C,et al.Differential uptake of functionalized polystyrene nanoparticles by human macrophages and a monocytic cell line[J]. ACS nano,2011,5(3):1657-69.
15.Chithrani BD,Ghazani AA, Chan WC. Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells[J].Nano Lett,2006,6(4):662-8.
16.Xia Y, You P, Xu F,et al.Novel Functionalized Selenium Nanoparticles for Enhanced Anti-Hepatocarcinoma Activity In vitro[J].Nanoscale Res Lett,2015,10 (1):1051.
17.Kuhn DA, Vanhecke D, Michen B,et al.Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages[J].Beilstein J Nanotechnol,2014,5:1625-36.
18.Wang Z, Tiruppathi C, Cho J,et al. Delivery of nanoparticle: complexed drugs across the vascular endothelial barrier via caveolae[J].IUBMB Life,2011,63 (8): 659-67.
19.Wang Z.Caveolae-mediated Delivery of Therapeutic Nanoparticles across Blood-endothelial Barrier[J].Austin J Anal Pharm Chem,2014,1(4).
20.Bengali Z, Rea JC,Shea LD.Gene expression and internalization following vector adsorption to immobilized proteins: dependence on protein identity and density[J].J Gene Med,2007,9(8):668-78.
21.Nobes C, Marsh M.Dendritic cells: new roles for Cdc42 and Rac in antigen uptake?[J].Curr Biol,2000,10(20):R739-41.
22.Palaniyandi K, Pockaj BA, Gendler SJ,et al.Human Breast Cancer Stem Cells Have Significantly Higher Rate of Clathrin-Independent and Caveolin-Independent Endocytosis than the Differentiated Breast Cancer Cells[J].J Cancer Sci Ther,2012,4(7):214-222.
23.Kong H, Zhang Y, Li Y,et al. Size-dependent cytotoxicity of nanocarbon blacks[J].Int J Mol Sci,2013,14 (11):22529-43.
24.Jiang L, Li X, Liu L,et al.Cellular uptake mechanism and intracellular fate of hydrophobically modified pullulan nanoparticles[J].Int J Nanomedicine, 2013,8:1825-34.
25.Ha HK, Kim JW, Lee MR,et al.Cellular Uptake and Cytotoxicity of beta-Lactoglobulin Nanoparticles: The Effects of Particle Size and Surface Charge[J].Asian-Australas J Anim Sci,2015,28(3):420-7.
26.Hanada S, Fujioka K, Inoue Y,et al.Cell-based in vitro blood-brain barrier model can rapidly evaluate nanoparticles' brain permeability in association with particle size and surface modification[J].Int J Mol Sci,2014,15(2):1812-25.
27.Kong H, Zhang Y, Li Y,et al.Size-dependent cytotoxicity of nanocarbon blacks[J].Int J Mol Sci,2013,14(11):22529-43.
28.Landgraf L, Muller I, Ernst P,et al.Comparative evaluation of the impact on endothelial cells induced by different nanoparticle structures and functionalization[J].Beilstein J Nanotechnol,2015,6:300-12.
29.Karaman DS, Desai D, Senthilkumar R,et al.Shape engineering vs organic modification of inorganic nanoparticles as a tool for enhancing cellular internalization[J].Nanoscale Res Lett,2012,7(1):358.
30.Dausend J,?Musyanovych A,?Dass M.,Uptake?mechanism?of?oppositely charged?fluorescent?nanoparticles?in?HeLa?cells[J].Macromol Biosci,2008,8(12):1135-43.
31.Vighi E,Leo E.Studying the in vitro behavior of cationic solid lipid nanoparticles as a nonviral vector[J].Int J Nanomedicine,2012,7(1):9-12.
32.Sahay G, Kim JO, Kabanov A V,et al. The exploitation of differential endocytic pathways in normal and tumor cells in the selective targeting of nanoparticulate chemotherapeutic agents[J].Biomaterials,2010,31(5):923-33.
33.Qaddoumi MG, Ueda H,Yang J,et al. The characteristics and mechanisms of uptake of PLGA nanoparticles in rabbit conjunctival epithelial cell layers[J].Pharm Res,2004,21(4):641-8.
34.Hanot CC, Choi YS, Anani TB,et al.Effects of Iron-Oxide Nanoparticle Surface Chemistry on Uptake Kinetics and Cytotoxicity in CHO-K1 Cells[J].Int J Mol Sci,2016,17(1).
35.Ruenraroengsak P, Tetley TD. Differential bioreactivity of neutral, cationic and anionic polystyrene nanoparticles with cells from the human alveolar compartment: robust response of alveolar type 1 epithelial cells[J].Part Fibre Toxicol,2015,12:19.
36.Yang H, Lou C, Xu M,et al.Investigation of folate-conjugated fluorescent silica nanoparticles for targeting delivery to folate receptor-positive tumors and their internalization mechanism[J].Int J Nanomedicine ,2011,6:2023-32.
37.Guedj AS, Kell AJ, Barnes M,et al. Preparation, characterization, and safety evaluation of poly(lactide-co-glycolide) nanoparticles for protein delivery into macrophages[J].Int J Nanomedicine,2015,10:5965-79.
38.Echevarria-Uraga JJ, Garcia-Alonso I, Plazaola F,et al.Study of the intra-arterial distribution of Fe(3)O(4) nanoparticles in a model of colorectal neoplasm induced in rat liver by MRI and spectrometry[J].Int J Nanomedicine, 2012,7:2399-410.

[1]	许珂张圆缪伟男陈文静. 上气道分析的研究进展[J]. , 2018, 38(6): 573-576.
[2]	李雪琦. 口腔正畸治疗患者下颌第三磨牙状态改变的相关危险因素分析[J]. , 2018, 38(5): 445-448.
[3]	沈红沈家平钱岷江. 南京市低龄儿童龋风险的影响因素研究[J]. , 2018, 38(12): 1128-1131.
[4]	范佳燕张玲傅柏平. 嵌体临床疗效的影响因素[J]. , 2018, 38(10): 956-960.
[5]	刘芳芳王志峰朱丽娜郝丹蓝菁. 济南市3~6岁儿童患龋风险预测及影响因素分析[J]. , 2017, 37(3): 245-247.
[6]	王靖宇王秀梅. 氧化锌纳米颗粒抗菌活性在医学中的应用及研究进展[J]. , 2017, 37(11): 1045-1048.
[7]	罗佳杨森. 唾液腺黏液表皮样癌的预后及影响因素分析[J]. , 2017, 37(1): 65-68.
[8]	陈玲. 妊娠妇女孕前口腔检查的调查状况及影响因素分析[J]. , 2016, 36(1): 74-76.
[9]	许正元常阳阳伍翔谢广平. 羟基磷灰石纳米颗粒对BMP-2的体外吸附和释放[J]. , 2015, 35(7): 517-520.
[10]	汤辉陈汉帮徐阳周怡章非敏夏阳. LbL法制备磁性纳米复合纤维膜生物相容性的研究[J]. , 2015, 35(5): 321-325.
[11]	邹茵,潘永初,王林. 上气道在正畸学中的研究进展[J]. , 2012, 32(8): 499-501.
[12]	姚国栋,江银华. 正畸治疗中牙根吸收的影响因素[J]. , 2012, 32(8): 502-504.
[13]	成凡平,林焕彩,武玉海. 影响妊娠妇女孕前口腔检查的多因素综合分析[J]. , 2012, 32(7): 433-436.
[14]	王娟王金平周荣静申丽丽盖菁菁. 上颌前磨牙的根管系统解剖形态特点及研究方法[J]. , 2012, 32(4): 0-0.
[15]	王娟；王金平；周荣静；申丽丽；盖菁菁. 上颌前磨牙的根管系统解剖形态特点及研究方法的研究进展[J]. , 2012, 32(4): 244-246.