三维打印超顺磁性氧化铁纳米组织工程支架

›› 2017, Vol. 37 ›› Issue (1): 6-10.

三维打印超顺磁性氧化铁纳米组织工程支架

周怡¹,胡姝颖¹,陈汉帮¹,夏阳²,秦天牧¹,章非敏³

1. 南京医科大学口腔疾病研究江苏省重点实验室
2. 南京医科大学附属口腔医院
3. 南京医科大学口腔医学院

收稿日期:2016-08-08 修回日期:2016-09-02 出版日期:2017-01-28 发布日期:2017-01-17
通讯作者: 章非敏 E-mail:fmzhang@njmu.edu.cn
基金资助:
国家自然科学基金;江苏省自然科学基金;中国博士后基金;江苏省博士后基金;江苏高校优势学科建设工程资助项目

Three dimensional printing SPIONs hydrogel scaffolds for tissue engineering

Received:2016-08-08 Revised:2016-09-02 Online:2017-01-28 Published:2017-01-17

摘要/Abstract

摘要： 目的　利用先进的三维打印技术，制备含有超顺磁性氧化铁纳米颗粒（superparamagnetic iron oxide nanoparticles，SPIONs）的支架，以期获得生物相容性更佳的组织工程支架。方法　通过三维打印的方法分别制备添加以及不添加SPIONs的明胶和海藻酸钠水凝胶支架，用体视显微镜、透射电镜、振动样品磁强计以及流式细胞仪对其形貌、磁学性能和生物学性能进行比较。结果　体视显微镜显示通过三维打印的方法可以制备出形态规则、孔径均匀的三维支架，透射电镜显示SPIONs在水凝胶基体中均匀分布。振动样品磁强计结果显示添加了SPIONs的支架具有超顺磁性。流式细胞仪检测结果显示实验组支架细胞增殖指数升高，具有促进细胞增殖的作用，表现出更好的生物相容性。结论　利用三维打印的方法可以成功制备出含SPIONs的组织工程支架，且该新型支架表现出更加优良的生物相容性。

关键词: 三维打印, 超顺磁性氧化铁, 组织工程, 支架

Abstract: Objective To fabricate scaffolds containing superparamagnetic iron oxide nanoparticles（SPIONs） by 3D printing technology in order to make scaffolds with better biocompatibility for tissue engineering. Methods Gelatin-alginate hydrogel scaffolds adding SPIONs and not adding SPIONs were both fabricated by 3D printing technology. Stereomicroscope, transmission electron microscope（TEM）,vibrating sample magnetometer（VSM）and flow cytometry（FCM）were used to characterize the surface topography, internal structure, magnetic properties and biological properties. Results Stereomicroscope showed that scaffolds with regular structure and uniform pore could be successfully fabricated by 3D printing technology, and TEM showed that SPIONs were distributed evenly in the hydrogel matrix .The VSM results showed that scaffolds containing SPIONs exhibited superparamagnetism. The FCM results showed that compared with the control scaffolds, the cell proliferation index in the scaffolds in experimental group elevated, and the magnetic scaffolds could promote cell proliferation, which indicated that the magnetic ones had better biocompatibility. Conclusion Tissue engineering scaffolds can be successfully fabricated by 3D printing technology and scaffolds with SPIONs show better compatibility compared with those without SPIONs.

Key words: 3D printing, superparamagnetic iron oxide nanoparticles, tissue engineering, scaffolds

中图分类号:

R783.1

周怡胡姝颖陈汉帮夏阳秦天牧章非敏. 三维打印超顺磁性氧化铁纳米组织工程支架[J]. , 2017, 37(1): 6-10.

参考文献

[1]Langer R,Vacanti JP.T5100 engineering[J].Science,1993,260(5100):920-926
[2]Sarker B,Hum J,Nazhat SN,et al.Combining collagen and bioactive glasses for bone t2 engineering:a review[J]..Adv Healthc Mater,2015,4(2):176-194
[3]Yan LP,Oliveira JM,Oliveira AL,et al.Macro/microporous silk fibroin scaffolds with potential for articular cartilage and meniscus t1 engineering applications[J].Acta Biomat,2012,8(1):289-301
[4]Bhardwaj N,Kundu SC.Chondrogenic differentiation of rat MSCs on porous scaffolds of silk fibroin/chitosan blends[J].Biomaterials,2012,33(10):2843-2857
[5]Sundraram S,Niklason LE.Smooth muscle and other cell sources for human blood vessel engineering[J].Cell T1/2 Organs,2012,195(1/2):15-25
[6]Li Q,Tao L,Chen B,et al.Extrahepatic bile duct regeneration in pigs using collagen scaffolds loaded with human collagen-binding bFGF[J].Biomaterials,2012,33(17):4298-4308
[7]Miyazawa M,Aikawa M,Okada K,et al.Regeneration of extrahepatic bile ducts of t1 engineering with a bioabsorbable polymer[J].J Artif Organs,2012,15(1):26-31
[8]Pan JF,Liu NH,Sun H,et al.Preparation and characterization of electrospun PLCL/poloxamer nanofibers and dextran/gelatin hydrogels for skin t11 engineering[J]..PLoS ONE,2014,9(11):e112885-
[9]Sachlos E,Czernuszka JT.Making t engineering scaffolds workReview:The application of solid freeform fabrication technology to the production of t engineering scaffolds[J]..Eur Cell Mater,2015,5:29-39
[10]Yang SF,Leong KF,Du ZH,et al.The design of scaffolds for use in t6 engineeringPart 1.Traditional factors[J]..T6 Eng,2001,7(6):679-689
[11]李青峰.3-D打印技术在整形外科的应用[J].中国修复重建外科杂志,2014,28(3):266-267
[12]王燎,戴尅戎.骨科个体化治疗与3D打印技术[J].医用生物力学,2014,9(3):193-199
[13]Sun W,Li J,Li Q,et al.Clinical effectiveness of hemipelvic reconstruction using computer-aided custom-made prostheses after resection of malignant pelvic tumors[J].J Arthroplasty,2011,26(8):1508-1513
[14]Tu TY,Wang Z,Bai J,et al.Rapid prototyping of concave microwells for the formation of 3D multicellular cancer aggregates for drug screening[J].Adv Healthc Mater,2014,3(4):609-616
[15]Lee SJ,Heo DN,Park JS,et al.Characterization and preparation of bio-tubular scaffolds for fabricating artificial vascular grafts by combining electrospinning and a 3D printing system[J].Phys Chem Chem Phys,2015,17(5):2996-2999
[16]Meng J,Zhang Y,Qi X,et al.Paramagnetic nanofibrous composite films enhance the osteogenic response of pre-osteoblast cells[J].Nanoscale,2010,2(12):2565-2569
[17]Meng J,Xiao B,Zhang Y,et al.Super-paramagnetic responsive nanofibrous scaffolds under static magnetic field enhance osteogenesis for bone repair in vivo[J].Sci Rep,2013,3:2655-
[18]Bose S,Vahabzadeh S,Bandyopadhyay A.Bone t12 engineering using 3D printing[J].Mater Today,2013,16(12):496-504
[19]Bandyopadhyay A,Bose S,Das S.3D pringting of biomaterials[J].MRS Bulletin,2015,40(2):108-115
[20]van Lenthe GH,Hagenmüller H,Bohner M,et al.Nondestructive micro-computed tomography for biological imaging and quantification of scaffold-bone interaction in vivo[J].Biomaterials,2007,(28):2479-2490
[21]Jeon H,Simon CG,Kim G.A mini-review:cell response to microscale,nanoscale,and hierarchical patterning of surface structure[J].J Biomed Materials Res Part B:Appl Biomater,2014,102(7):1580-1594
[22]Park S,Im GI.Stem cell responses to nanotopography[J].J Biomed Mater Res Part A,2015,103(3):1238-1245
[23]Singh P,YashRoy RC,Hogue M.Augmented bone-matrix formation and osteogenesis under magnetic field stimulation in vivo XRD,TEM and SEM investigations[J].Indian J Biochem Biophys,2006,43(3):167-172
[24]Bock N,Riminucci A,Dionigi C,et al.A novel route in bone t3 engineering:magnetic biomimetic scaffolds[J].Acta Biomater,2010,6(3):786-796
[25]Ormerod MG.Investigating the relationship between the cell cycle and apoptosis using flow cytometry[J].J Immunol Methods,2002,265:73-80