BMSCs和FG作为种子细胞和支架材料修复大鼠牙槽骨缺损的效果

›› 2017, Vol. 37 ›› Issue (4): 307-310.

BMSCs和FG作为种子细胞和支架材料修复大鼠牙槽骨缺损的效果

刘杨¹,孙大磊²,王毅³,胡磊⁴,赵玺⁵,曹卫彬¹

1. 新疆医科大学第六附属医院口腔科
2. 新疆医科大学第一附属医院儿牙科
3. 新疆医科大学附属中医医院口腔科
4. 乌鲁木齐市友谊医院口腔科
5. 乌鲁木齐市口腔医院

收稿日期:2016-08-22 修回日期:2016-09-12 出版日期:2017-04-28 发布日期:2017-04-11
通讯作者: 赵玺 E-mail:417955104@qq.com
基金资助:
新疆维吾尔自治区自然科学基金

Effects of BMSCs and FG as seed cells and scaffold materials on repairing alveolar bone defect in rats

Received:2016-08-22 Revised:2016-09-12 Online:2017-04-28 Published:2017-04-11

摘要/Abstract

摘要： 目的探讨骨髓间充质干细胞（BMSCs）和纤维蛋白胶（FG）作为种子细胞和支架材料修复大鼠牙槽骨缺损的效果。方法选取SD大鼠30只，建立牙槽骨缺损模型，随机分为观察组、阴性对照组和空白对照组，每组10只。观察组植入BMSCs和FG修复大鼠牙槽骨缺损，阴性对照组植入FG，空白对照组不植入，采用HE染色及MicroCT扫描观察各组成骨效应。结果观察组植入6周后，骨愈合情况良好，愈合面较平整；阴性对照组和空白对照组骨缺损仍存在，无明显成骨；观察组新生骨面积百分比为（76.23±7.11）%，明显高于阴性对照组和空白对照组，差异比较有统计学意义（P＜0.05）；MicroCT横断扫描显示：观察组骨缺损处新骨形成，骨缺损基本愈合，与周边无明显界限，阴性对照组和空白对照组无明显成骨，骨缺损仍明显。结论 BMSCs和FG作为种子细胞和支架材料修复大鼠牙槽骨缺损有较好的效果，提高了新骨生成量，是牙槽骨缺损修复的理想材料。

关键词: 骨髓间充质干细胞, 纤维蛋白胶, 牙槽骨缺损, 大鼠

Abstract: Objective To investigate the effects of bone marrow mesenchymal stem cells (BMSCs) and fibrin glue (FG) as seed cells and scaffold materials in repairing alveolar bone defect in rats. Methods 30 SD rats were selected to establish the alveolar bone defect model， randomly divided into observation group， negative control group and blank control group， with 10 rats in each group. In the observation group， BMSCs and FG were implanted to repair the alveolar bone defect of the rats; while the negative control group was implanted with FG， and the blank control group was not implanted. HE staining and MicroCT scanning were used to observe the osteogenesis effect of each group. Results After 6 weeks in the observation group， the bone healing was good， and the healing surface was smooth; while in the negative control group and blank control group， the bone defect still existed， and there was no obvious bone formation. The percentage of new bone area in the observation group was (76.23±7.11)%， significantly higher than that of the negative control group and blank control group， and the difference was statistically significant (P<0.05). MicroCT scanning showed that in the observation group， new bone formed in the area where bone defect existed， bone defect basically healed， and there was no obvious boundaries around. The negative control group and blank control group had no obvious osteogenesis， and the bone defect was still obvious. Conclusion BMSCs and FG as seed cells and scaffold materials for repairing alveolar bone defects have good effects， which can improve the new bone quantity， and are the ideal materials for the repair of alveolar bone defects.

Key words: bone marrow mesenchymal stem cells, fibrin glue, alveolar bone defect, rat

中图分类号:

R782.4

刘杨孙大磊王毅胡磊赵玺曹卫彬. BMSCs和FG作为种子细胞和支架材料修复大鼠牙槽骨缺损的效果[J]. , 2017, 37(4): 307-310.

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