关键词: 人牙髓干细胞, 组织工程, I 型胶原 , 肽段水凝胶

Abstract: To evaluate the effects of two kinds of injectable gel-like scaffolds with different concentrations on the proliferation of dental pulp stem cells in dental pulp tissue engineering. Methods:Different concentrations of type I collagen and peptide hydrogel scaffolds were prepared, and dental pulp stem cells were inoculated on each group of scaffolds respectively. Cell proliferation was detected by CCK-8 assay; cell number and morphology were observed by staining with dead cells. Results The dental pulp stem cells inoculated with different concentrations of scaffolds all grew well. 1% collagen scaffolds and 0.25% hydrogel scaffolds could promote the proliferation of dental pulp stem cells. The viable cells stained dental pulp stem cells Both scaffold materials at various concentrations grew well, and the number of cells showed an increasing trend with the prolongation of culture time. The cells were fully extended and showed a spindle type.

Key words: Human dental pulp stem cells, Tissue engineering, type I collagen, peptide hydrogel