关键词: 丝素蛋白, 3D打印, 纳米羟基磷灰石

Abstract: Objective　 To prepare silk fibroin(SF)/polyvinyl alcohol(PVA) / nano-hydroxyapatite (nHA) scaffold and polyvinyl alcohol(PVA) / nano-hydroxyapatite (nHA) scaffold using 3D printing technology and detect their performances. Methods　3D printing technology was used to print SF/PVA/nHA scaffold and PVA/nHA scaffold. Porosity, morphology, mechanical properties and cytotoxicity of different scaffolds were detected. Results　 ①Under scanning electron microscope, SF/PVA/nHA scaffold had regular scaffold structure and clear network structure, exhibited continuous communicating branch, well-connected overlap joint, and uniform scaffold space. Under the same multiples, the network structure continuity of PVA/nHA scaffold was poorer. ②Compressive mechanical properties: Under the same stress(10 Mpa), the strain of SF/PVA/nHA scaffold was larger than that of PVA/nHA scaffold. ③The pore size of group SF/PVA/nHA scaffold was significantly larger than PVA/nHA scaffold. ④Cell toxicity detection: The deformation of SF/PVA/nHA scaffold ,under the same stress(10 MPa),was larger than PVA/nHA scaffold. There was no significant difference in cell appreciation rate between two groups of scaffolds at different time points. Conclusions　3D printing SF/PVA/nHA scaffold and PVA/nHA scaffold hold good physicochemical performance and cytocompatibility.

Key words: silk fibroin solution, 3D printing, nano-hydroxyapatite