过氧化氢银离子消毒剂控制微生物污染的效果检测

摘要/Abstract

摘要： 目的研究过氧化氢银离子消毒剂对江苏省口腔医院牙周科的口腔科综合治疗台水路系统的微生物污染的控制效果。方法选取牙周科4台椅位进行3 d/次的管道消毒，共6 d。消毒前后分别采集三用枪，漱口位，超声洁治机柄出水口的水样10 mL在R2A培养基上进行菌落培养，统计消毒前后菌落密度，数据用SPSS 19.0进行统计分析。结果消毒前三用枪、漱口位、高速手机3个位点出水的平均菌落密度分别为34425、13116.67、42858.33 CFU/mL，无统计学差异。消毒之后灭菌率在92~100%之间，且三个位点的消毒效果无统计学差异。合格率在消毒结束当天早晨平均为91.67%，但消毒结束后第三天早晨，合格率仅为45.83%。结论应用过氧化氢银离子消毒剂对口腔科综合治疗台水路系统微生物污染改善效果显著，但持久性方面有欠缺。

关键词: 口腔科综合治疗台水路系统, 消毒剂, 过氧化氢, 微生物

Abstract: Objective To observe the contamination control of microorganisms in dental unit waterlines after using the disinfectant agent containing hydrogen peroxide and silver ion. Methods 4 dental units in the department of periodontics were chosen to install disinfection devices and disinfected every three days in six days. before and after the disinfectant,10ml water samples were collected from the operator’s three-in-one air/water syringe, ultrasonic scaler waterlines and water for rinsing the mouth, cultured on R2A ager plates, recorded the Colony-Forming Units, compared them with 500 CFU/ml, analyzed the data with SPSS 19.0.Results Before the application of disinfectant, the bacterial colony reached to 34425CFU/ml from air/water syringe, 42858.33CFU/ml from ultrasonic scalers waterline and 13116.67CFU/ml from rinse water, there is no significant difference of bacterial colony between water samples before disinfecting. After using the disinfectant agent, the sterilization rates were from 92% to 100% and no significant difference was discovered between them. However, on the third morning after disinfecting, the level of bacterial colony has a clear recovery. Conclusion The disinfection effect of disinfectant within hydrogen peroxide and silver ion is significant but the maintenance of microorganisms in dental unit waterlines is not so good.

Key words: dental unit waterline system, disinfectant, hydrogen peroxide, microorganism

中图分类号:

R187.2

赵奇韬周如玉梅予锋王娟. 过氧化氢银离子消毒剂控制微生物污染的效果检测[J]. , 2018, 38(8): 694-698.

参考文献

[1].［参　考　文　献］
[2]O' Donnell MJ, Boyle M, Swan J, et al.A centralised,automated dental hospital water quality and biofilm management system using neutral Ecasol maintains dental unit waterline output at better than potable quality: a 2-year longitudinal study[J].J Dent, 2009, 37(10):748-762
[3]Ji XY, Fei CN, Zhang Y, et al.Evaluation of bacterial contamination of dental unit waterlines and use of a newly designed measurement device to assess retraction of a dental chair unit[J].Int Dent J, 2016, 66(4):208-214
[4]Szymanska J.Exposure to bacterial endotoxin during conservative dental treatment[J].Ann Agric Environ Med, 2005, 12(1):137-139
[5]Huntington MK, Williams JF, Mackenzie CD.Endotoxin contamination in the dental surgery[J].J Med Microbiol, 2007, 56(Pt 9):1230-1234
[6]O' Donnell MJ, Shore AC, Coleman D C.A novel automated waterline cleaning system that facilitates effective and consistent control of microbial biofilm contamination of dental chair unit waterlines: a one-year study[J].J Dent, 2006, 34(9):648-661
[7]辛鹏举, 黄凝, 孙惠惠, 等.微酸性电解水对口腔综合治疗台水路持续消毒效果研究[J]. 中国消毒学杂志, 2017(05):422-425.
[8]刘本来, 陈敏, 李国庆, 等.乌鲁木齐市医疗机构口腔综合台用水污染调查分析[J]. 世界最新医学信息文摘, 2016(30):141-187.
[9]高丽君, 赵奇, 李素华, 等.河南省医疗机构口腔科综合治疗台不同时段水污染状况调查[J]. 中华医院感染学杂志, 2016(21):4990-4991.
[10]王一梅, 刘德丰, 许慧琼, 等.弱酸性次氯酸水溶液用于口腔综合治疗台水路消毒的效果观察[J]. 中国消毒学杂志, 2016(11):1041-1043.
[11]张元, 平逸帆, 时玉洁, 等.影响口腔综合治疗台管道水质的相关因素研究[J]. 南京医科大学学报(自然科学版), 2016(07):846-849.
[12]周如玉, 平逸帆, 张元, 等.口腔综合治疗台水路系统中假单胞菌属污染状况的检测[J]. 南京医科大学学报(自然科学版), 2017(04):452-457.
[13]Ricci M L, Fontana S, Pinci F, et al.Pneumonia associated with a dental unit waterline[J].Lancet, 2012, 379(9816):684-
[14]Peralta G, Tobin-D' Angelo M, Parham A, et al.Notes from the Field: Mycobacterium abscessus Infections Among Patients of a Pediatric Dentistry Practice--Georgia,2015[J].MMWR Morb Mortal Wkly Rep, 2016, 65(13):355-356
[15]Cobb CM, Martel CR, McKnight S, et al.How does time-dependent dental unit waterline flushing affect planktonic bacteria levels?[J].J Dent Educ, 2002, 66(4):549-555
[16]Fiehn NE, Larsen T.The effect of drying dental unit waterline biofilms on the bacterial load of dental unit water[J].Int Dent J, 2002, 52(4):251-254
[17]Berlutti F, Testarelli L, Vaia F, et al.Efficacy of anti-retraction devices in preventing bacterial contamination of dental unit water lines[J].J Dent, 2003, 31(2):105-110
[18]Luo J, Porteous N, Sun Y.Rechargeable biofilm-controlling tubing materials for use in dental unit water lines[J].ACS Appl Mater Interfaces, 2011, 3(8):2895-2903
[19]Tuttlebee CM, O' Donnell MJ, Keane CT, et al.Effective control of dental chair unit waterline biofilm and marked reduction of bacterial contamination of output water using two peroxide-based disinfectants[J].J Hosp Infect, 2002, 52(3):192-205
[20]McDowell JW, Paulson DS, Mitchell JA.A simulated-use evaluation of a strategy for preventing biofilm formation in dental unit waterlines[J].J Am Dent Assoc, 2004, 135(6):799-805
[21]Gawande PV, LoVetri K, Yakandawala N, et al.Antibiofilm activity of sodium bicarbonate,sodium metaperiodate and SDS combination against dental unit waterline-associated bacteria and yeast[J].J Appl Microbiol, 2008, 105(4):986-992
[22]Kohno S, Kawata T, Kaku M, et al.Bactericidal effects of acidic electrolyzed water on the dental unit waterline[J].Jpn J Infect Dis, 2004, 57(2):52-54
[23]Linley E, Denyer S P, McDonnell G, et al.Use of hydrogen peroxide as a biocide: new consideration of its mechanisms of biocidal action[J].J Antimicrob Chemother, 2012, 67(7):1589-1596
[24]Smith C A, Pepose J S.Disinfection of tonometers and contact lenses in the office setting: are current techniques adequate?[J].Am J Ophthalmol, 1999, 127(1):77-84
[25]Barbut F, Menuet D, Verachten M, et al.Comparison of the efficacy of a hydrogen peroxide dry-mist disinfection system and sodium hypochlorite solution for eradication of Clostridium difficile spores[J].Infect Control Hosp Epidemiol, 2009, 30(6):507-514
[26]Szymanska J.Antifungal efficacy of hydrogen peroxide in dental unit waterline disinfection[J].Ann Agric Environ Med, 2006, 13(2):313-317
[27]Yang HL, Lin JC, Huang C.Application of nanosilver surface modification to RO membrane and spacer for mitigating biofouling in seawater desalination[J].Water Res, 2009, 43(15):3777-3786
[28]Park H J, Kim J Y, Kim J, et al.Silver-ion-mediated reactive oxygen species generation affecting bactericidal activity[J].Water Res, 2009, 43(4):1027-1032
[29]Gitipour A, Al-Abed SR, Thiel S W, et al.Nanosilver as a disinfectant in dental unit waterlines: Assessment of the physicochemical transformations of the AgNPs[J]. Chemosphere, 2017, 173:245-252.
[30]Luo J, Sun Y.Acyclic N-halamine-based biocidal tubing: preparation,characterization,and rechargeable biofilm-controlling functions[J].J Biomed Mater Res A, 2008, 84(3):631-642