微弧氧化钛表面对2型糖尿病大鼠骨髓基质干细胞成骨分化与种植体骨结合的影响

›› 2019, Vol. 39 ›› Issue (12): 1062-1067.

微弧氧化钛表面对2型糖尿病大鼠骨髓基质干细胞成骨分化与种植体骨结合的影响

钱海馨,张富强

上海第九人民医院

收稿日期:2018-07-27 修回日期:2018-11-23 出版日期:2019-12-28 发布日期:2019-12-31
通讯作者: 张富强 E-mail:fqzhang9hospital@aliyun.com

The effect of micro arc oxidation treated Ti surface on osteogenic differentiation of bone marrow mesenchymal stem cells and osseointegration of induced type 2 diabetes mellitus rats.

Received:2018-07-27 Revised:2018-11-23 Online:2019-12-28 Published:2019-12-31

摘要/Abstract

摘要： 研究微弧氧化钛表面对2型糖尿病大鼠骨髓基质干细胞成骨分化与种植体骨结合的影响。方法对TC4表面微弧氧化处理获得微纳米表面，高糖高脂饮食结合腹腔内注射链脲佐菌素溶液建立2型糖尿病大鼠模型，分离大鼠骨髓基质干细胞。接种细胞到微弧氧化钛表面，观察细胞附着形态、增殖活性、碱性磷酸酶（ALP）活性和成骨分化相关基因。植入微弧氧化种植体到2型糖尿病患者股骨内，荧光素标记新骨形成。采用激光共聚焦显微镜（EDS）观察新骨形成速度，苦味酸品红染色观察骨结合率。采用配对t检验（SPSS 2.0软件）分析实验组和对照组之间细胞增殖、成骨分化相关基因表达、碱性磷酸酶半定量活性、新骨形成量和骨结合率差异（α=0.05）。结果 TC4经酸蚀处理后形成粗糙表面，经微弧氧化处理后可以观察到微纳米结构的孔隙。EDS检测到微弧氧化后的钛表面有钠、硅和磷等元素。微弧氧化钛表面的细胞伸展形态由于对照组，ALP活性增加，成骨分化基因表达上调。动物实验显示微弧氧化钛表面种植体新骨形成速度在前两个月快于对照组，骨结合率高于对照组。结论对TC4表面微弧氧化处理后可以促进2型糖尿病大鼠骨髓基质干细胞成骨分化与种植体骨结合。

关键词: 微弧氧化，2型糖尿病，成骨分化，骨结合

Abstract: Objective: The aim of the study was to evaluate the effect of micro arc oxidation treated Ti surface on osteogenic differentiation of bone marrow mesenchymal stem cells and osseointegration of induced type 2 diabetes mellitus rats. Materials and methods. TC4 surface was treated by micro arc oxidation to create a hierarchy structure. Type 2 diabetic rat model was induced by feeding with high-fat diet and followed by injection of streptozotocin. The BMSCs of diabetic rat were cultured in vitro. The cells were seeded on the treated surface to evaluate the effect of the surface on osteogenic differentiation. The implant with the surface was inserted in the diabetic rats’ femurs to evaluate the influence on new bone formation and bone implant contact. Results. In vitro study revealed the treated structure could upregulate the expressions of osteogenic-related markers. In vivo study revealed the enhanced bone regeneration, implant bone contact in diabetic rats. Conclusions. The advantages of micro arc oxidation treated surface could improve osteogenic differentiation and osseointegration in diabetic environment.

Key words: micro-oxidation, type 2 diabetes ,osteogenic differentiation, osseointegration

钱海馨张富强. 微弧氧化钛表面对2型糖尿病大鼠骨髓基质干细胞成骨分化与种植体骨结合的影响[J]. , 2019, 39(12): 1062-1067.

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