复合骨修复材料HAPw/nmZnO-nmCaO的抗菌性能研究

›› 2019, Vol. 39 ›› Issue (3): 199-202.

复合骨修复材料HAPw/nmZnO-nmCaO的抗菌性能研究

李靖¹,张文云²,常加贺¹,袁艳波¹,和丽佳³,苏军³,赵强¹,陈江容⁴

1. 解放军昆明总医院
2. 成都军区昆明总医院口腔科
3. 成都军区昆明总医院
4. 昆明医科大学

收稿日期:2018-09-19 修回日期:2018-10-19 出版日期:2019-03-28 发布日期:2019-03-22
通讯作者: 李靖 E-mail:515384248@qq.com
基金资助:
羟基磷灰石晶须的抗菌改性及机理研究

Antibacterial properties research on HAPw/nmZnO-nmCaO composite bone restorative material in vitro

Received:2018-09-19 Revised:2018-10-19 Online:2019-03-28 Published:2019-03-22
Contact: Jing LI E-mail:515384248@qq.com

摘要/Abstract

摘要： 目的：研究HAPw/nmZnO-nmCaO对金黄色葡萄球菌、铜绿假单胞菌的体外抗菌性能。方法：配制OD595=0.1的金黄色葡萄球菌、铜绿假单胞菌悬液。用灭菌纯水配制浓度为1mg/ml的HAPw、Bio-oss、HAPw/nmZnO-nmCaO材料悬浊液。A组：5ml培养基+1ml HAPw/nmZnO-nmCaO+200ul菌悬液；B组：5ml培养基+1ml HAPw+200ul菌悬液；C组：5ml培养基+1ml Bio-oss+200ul菌悬液；D空白组：5ml培养基+1ml灭菌纯水+200ul菌悬液。各组37℃恒温培养20h，取样进行浓度梯度稀释。稀释后的菌液取100ul涂布于琼脂平板，37℃恒温培养20h行平板菌落计数。结果：金黄色葡萄球菌和铜绿假单胞菌HAPw/nmZnO-nmCaO组的菌落数量少于HAPw、Bio-oss、空白对照组（P＜0.001），HAPw、Bio-oss、空白对照组三组之间无差异（P＞0.05）。结论：HAPw/nmZnO-nmCaO对金黄色葡萄球菌、铜绿假单胞菌的生长均有抑制作用，对金黄色葡萄球菌的抑制作用强于铜绿假单胞菌。

关键词: HAPw/nmZnO-nmCaO, 无机抗菌剂, 抗菌性能

Abstract: Researched on antibacterial properties of HAPw/nmZnO-nmCaO against Staphylococcus aureus and Pseudomonas aeruginosa in vitro. Material and methods:Prepared Staphylococcus aureus and Pseudomonas aeruginosa suspension,OD595=0.1.HAPw、Bio-oss、HAPw/nmZnO-nmCaO material suspension prepared with 1mg/ml of sterile pure water.Group A: 5 ml medium + 1 ml HAPw/nmZnO-nmCaO + 200ul bacterial suspension; Group B: 5 ml medium + 1 ml HAPw + 200ul bacterial suspension; Group C: 5 ml medium + 1 ml Bio-oss + 200ul bacterial suspension; D control group: 5 ml medium + 1 ml sterilized pure water + 200 ul bacterial suspension.Each group was incubate 20h in thermostat with temperature of 37℃,then the samples were diluted with a concentration gradient. The diluted mycelium was coated on the agar plate with 100ul and incubate 20h in thermostat with temperature of 37℃,the plate colonies were counted..Results:Staphylococcus aureus and Pseudomonas aeruginosa HAPw/nmZnO-nmCaO group had fewer colonies than HAPw, Bio-ose, control group(P＜0.001),there was no siginificant difference between HAPw, Bio-oss, and control group(P＞0.05).Conclusions:HAPw/nmZnO-nmCaO inhibited the growth of Staphylococcus aureus and Pseudomonas aeruginosa. The inhibition of Staphylococcus aureus was stronger than that of Pseudomonas aeruginosa.

Key words: HAPw/nmZnO-nmCaO, inoganic antiseptic, antibacterial property

中图分类号:

R318.08

李靖张文云常加贺袁艳波和丽佳苏军赵强陈江容. 复合骨修复材料HAPw/nmZnO-nmCaO的抗菌性能研究[J]. , 2019, 39(3): 199-202.

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