口腔鳞状细胞癌预后相关基因标志物的分析

摘要/Abstract

摘要： ［摘要］目的通过生物信息分析途径对口腔鳞状细胞癌患者与正常人群的差异基因进行分析，从分子水平探讨关键基因以及参与的信号通路，初步探索口腔鳞状细胞癌发生、发展的基因标志物。方法从公共数据库基因表达数据库（GEO）中下载口腔鳞状细胞癌的相关芯片数据（GSE3524和GSE6631），筛选出口腔鳞状细胞癌组织和对照组织表达有显著差异的基因。并对其功能及预后进行分析。结果共筛选出129个差异表达基因，其中表达上调45个，下调84个，对其进行基因本体、京都基因与基因组百科全书和蛋白互作网络分析，发现分泌型焦磷酸蛋白（secreted phosphoprotein 1， SPP1）、金属基质蛋白酶（matrix metalloproteinase 1，MMP1）、丝氨酸蛋白酶抑制剂（serpin family E member 1，SERPINE1）、纤溶酶原激活剂（plasminogen activator urokinase，PLAU）处于基因核心节点位置。同时，根据这些关键基因表达水平的高低对口腔鳞状细胞癌患者进行分组，高表达组患者生存时间均低于低表达组，差异有统计学意义（PSPP1=0.045、PMMP1=0.046、PSERPINE1=0.0024和PPLAU=0.00049）。结论基因组学分析方法筛选出的关键基因和信号通路有助于研究口腔鳞状细胞癌发生、发展的机制，也进一步为治疗靶点及预后分子标志物的选择提供了依据。

关键词: 口腔鳞状细胞癌, 差异基因, 信号通路, 预后, 分子标志物

Abstract: Objective　To analyze the differentially expressed genes of patients with oral squamous cell carcinoma (OSCC) from normal people through biological information analysis, to discuss the key genes and pathways from molecular level, and to preliminarily investigate the gene landmarks of the occurrence and development of OSCC. Methods Differentially expressed genes (DEGs) between OSCC and control tissue samples from the GSE3524 and GSE6631 gene expression profile dataset were screened. The Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and protein-protein interaction (PPI) network analyses were performed. Furthermore, key genes on the survival of patients with OSCC were analyzed. Results A total of 129 DEGs were identified, including 45 up-regulated and 84 down-regulated genes. GO, KEGG and PPI network analyses were performed. In addition, the high expression of the hub genes from the PPI network (including secreted phosphoprotein 1, matrix metalloproteinase 1, serpin family E member 1 and plasminogen activator, urokinase; P= 0.045, 0.046, 0.0024 and 0.00049, respectively) was associated with a decrease in overall survival for patients with OSCC as identified using survival and expression data. Conclusion The identified hub genes and pathways may help to elucidate the molecular mechanisms of OSCC occurrence and development. Additionally, they may be useful as therapeutic targets or serve as novel biomarkers for OSCC prognosis prediction.

Key words: oral squamous cell carcinoma, differentially expressed genes, Signaling pathway, survival analysis, biomarker

中图分类号:

R739.8

汤辉杜月明陈奇峰张宇. 口腔鳞状细胞癌预后相关基因标志物的分析[J]. , 2019, 39(8): 679-684.

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