柚皮苷对脂多糖诱导的人牙周膜成纤维细胞增殖及炎症因子表达的影响

›› 2020, Vol. 40 ›› Issue (3): 198-203.

柚皮苷对脂多糖诱导的人牙周膜成纤维细胞增殖及炎症因子表达的影响

刘景

新疆医科大学第五附属医院

收稿日期:2019-05-06 修回日期:2019-06-17 出版日期:2020-03-28 发布日期:2020-03-31
通讯作者: 刘景 E-mail:duanchenfor@163.com
基金资助:
新疆维吾尔自治区自然科学基金项目

Effects of naringin on proliferation and expression of inflammatory cytokines in human periodontal ligament fibroblast induced by lipopolysaccharide

Received:2019-05-06 Revised:2019-06-17 Online:2020-03-28 Published:2020-03-31

摘要/Abstract

摘要： 摘要：目的探讨柚皮苷（naringin，NRG）对脂多糖诱导的人牙周膜成纤维细胞增殖及炎症因子表达的影响。方法以脂多糖（lipopolysaccharide，LPS）（100 μg?mL-1）诱导人牙周膜成纤维细胞（human periodontal ligament fibroblast，HPDLF）损伤，采用MTT法检测NRG对LPS诱导HPDLF损伤的保护作用，利用ELISA法和Western blotting法检测NRG对LPS诱导的HPDLF中白介素（interleukin，IL）?6、IL?8、IL?1β等炎症因子蛋白表达的影响，同时利用Real-time PCR方法检测NRG对LPS诱导的HPDLF组IL?6、IL?8、IL?1β等炎症因子基因表达的影响。结果经LPS(100 μg?mL-1）刺激后，HPDLF增殖受到抑制，与CON组比较，LPS组细胞存活率显著降低，差异具有统计学意义（P<0.05）；不同浓度的NRG（40，20，10μg?mL-1）可显著抑制LPS诱导的HPDLF损伤，并可降低细胞内炎症因子表达；与LSP组比较，LPS+NRG 40μg?mL-1组、LPS+ NRG 20μg?mL-1组、LPS+NRG 10μg?mL-1组HPDLF存活率显著提升，IL?6、IL?8、IL?1β等炎症因子蛋白及基因表达水平均显著降低，差异具有统计学意义（P<0.05）。结论 NRG对LPS诱导的HPDLF损伤具有保护作用，并可通过抑制LPS诱导的HPDLF中IL?6、IL?8、IL?1β等炎症因子蛋白及基因表达水平，促进HPDLF增殖。

关键词: 柚皮苷, 脂多糖, 人牙周膜成纤维细胞, IL?6, IL?8, IL?1β

Abstract: ABSTRACT: Objective To investigate the effects of naringin (NRG) on the proliferation and expression of inflammatory factors of human periodontal ligament fibroblast (HPDLF) induced by lipopolysaccharide (LPS). Methods: HPDLF was induced by lipopolysaccharide (LPS) (100 μg?mL-1). The protective effect of NRG on LPS induced HPDLF injury was detected by MTT assay. The effects of NRG on the expression of IL-6, IL-8, IL?1β and other inflammatory factors in LPS-induced HPDLF were detected by ELISA and Western blotting. The effects of NRG on the expression of IL-6, IL-8 and IL?1β in LPS-induced HPDLF were also detected by Real-time PCR. Results: The proliferation of HPDLF was inhibited by LPS (100 μg?mL-1). Compared with the CON group, the survival rate of cells in LPS group was significantly decreased (P<0.05). Different concentrations of NRG (40, 20, 10 μg?mL-1) could significantly inhibit LPS-induced HPDLF injury and reduce the expression of inflammatory factors in cells. (P<0.05). Compared with LSP group, the survival rate of HPDLF in LPS+NRG 40 μg?mL-1 group, LPS+NRG 20 μg?mL-1 group, LPS+NRG 10 μg?mL-1 group was significantly increased, and the expression levels of inflammatory factors such as IL 6, IL-8, IL?1β were significantly decreased, with significant difference (P<0.05). Conclusion: NRG has protective effect on LPS-induced HPDLF injury, and can promote the proliferation of HPDLF cells by inhibiting the expression levels of IL-6, IL-8, IL?1β and other inflammatory factors in LPS-induced HPDLF.

Key words: naringin, lipopolysaccharide, human periodontal ligament fibroblast, IL-6, IL-8, IL?1β

中图分类号:

R781.4

刘景. 柚皮苷对脂多糖诱导的人牙周膜成纤维细胞增殖及炎症因子表达的影响[J]. 口腔医学, 2020, 40(3): 198-203.

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