脂联素受体表达与牙周炎

摘要/Abstract

摘要： 　目的　研究健康人牙龈成纤维细胞（human gingival fibroblasts,HGFs）中脂联素受体mRNA的表达及肿瘤坏死因子-α（tumor necrosis factor alpha,TNF-α）、牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g) 脂多糖（lipopolysaccharide,LPS）、脂联素（Adiponectin，APN）对HGFs中脂联素受体1（adiponectin receptor 1 ,AdipoR1）、脂联素受体2（adiponectin receptor 2,AdipoR2）信使核糖核酸（messenger ribonucleic acid , mRNA）表达的影响，探讨牙周炎相关因素与HGFs脂联素受体表达的相关性。方法　运用组织块贴壁法体外培养健康HGFs, 反转录聚合酶链式反应（reverse transcription polymerase chain reaction,RT-PCR）法检测其脂联素受体的表达,将细胞随机分为4组,分别用TNF-α 50ng/ml，P.g LPS 0.1μg/ml，APN 1μg/ml，空白对照（10%FBS培养基）刺激,持续24小时,然后运用实时定量聚合酶链式反应（real time polymerase chain reaction ,real-time PCR）检测不同时间点（0、0.5h、12h、24h）HGFs中AdipoR1、AdipoR2 mRNA表达水平。结果　RT-PCR显示HGFs均表达AdipoR1和AdipoR2,其中AdipoR2条带较AdipoR1更亮, 与空白对照组相比，TNF-α下调了HGFs的AdipoR2 mRNA表达（P <0.05）,P.g LPS与APN明显上调了HGFs的AdipoR1/R2 mRNA的表达（P <0.05）。结论　脂联素受体（AdipoR1、AdipoR2）在人牙龈成纤维细胞中均有表达，且主要以AdipoR2为主，并且在炎症状态下，脂联素受体表达下降。

关键词: 牙周炎, 脂联素受体 , 脂联素, 肿瘤坏死因子α, 牙龈卟啉单胞菌脂多糖

Abstract: Objective　 To study the expression of adiponectin receptor mRNA in the human gingival fibroblasts(HGFs) of healthy subjects and the effect of tumor necrosis factor alpha,（TNF-α）, Porphyromonas gingivalis（P.g.）lipopolysaccharide (LPS) and adiponectin(APN )on the expression of adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2) mRNA in HGFs to investigate the correlation between the factors related to periodontitis and the expression of adiponectin receptors and. Methods　 The HGFs were cultured in vitro by tissue-adherent method, using reverse transcription polymerase chain reaction(RT-PCR) to detect the expression of adiponectin receptors， and the cells were randomly divided into 4 groups, stimulated with TNF-α50ng/ml ,P.g LPS 0.1mg/ml ,APN 1μg/ml, and a blank control (10% FBS medium) respectively ,lasting for 24 hours, then using real time polymerase chain reaction （real-time PCR） to detect the expression of adiponectin receptor (AdipoR1, AdipoR2) mRNA in HGFs at different time points (0, 0.5, 12, 24h). Results　 RT-PCR showed that HGFs expressed both AdipoR1 and AdipoR2, in which AdipoR2 bands had a higher luminance than AdipoR1, and TNF-a decreased AdipoR2 mRNA expression (p<0.05) in HGFs compared with the blank control group, P.g LPS and APN significantly raised the expression of AdipoR1 and AdipoR2 mRNA (p<0.05).Conclusions　 Adiponectin receptors (AdipoR1, AdipoR2) are both expressed in human gingival fibroblasts, and primarily the AdipoR2. In the condition of periodontal inflammation, the expression of adiponectin receptor decreased, therefore the effect of adiponectin decreased, auxiliary APN receptor agonist and other local drug treatment for the control of inflammation is of great significant to prevent aggravating the disease.

Key words: Periodontitis, Adiponectin recepteor, Adiponectin, TNF-α, Porphyromonas gingivalis lipopolysaccharide

王丽美支敏吕佩颖高鹏飞蔺晨杨艳艳王永兰. 脂联素受体表达与牙周炎[J]. 口腔医学, 2020, 40(3): 204-208.

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