Nd:YAG激光对鳞状细胞癌CAL-27细胞增殖的影响

›› 2015, Vol. 35 ›› Issue (5): 362-364.

Nd:YAG激光对鳞状细胞癌CAL-27细胞增殖的影响

马浩然¹,吴国英¹,李留炀²,尚进²,钱棱²,陶楠楠²,钱梦柯²,王培²,仇嘉颖²

1. 南京医科大学附属口腔医院-江苏省口腔医院
2. 南京医科大学

收稿日期:2014-11-07 修回日期:2015-01-08 出版日期:2015-05-28 发布日期:2015-05-13
通讯作者: 吴国英 E-mail:wuguoying888@163.com
基金资助:
江苏省高等学校大学生实践创新训练计划

The effect of Nd: YAG laser irradiation on squamous carcinoma CAL-27 cell proliferation

Received:2014-11-07 Revised:2015-01-08 Online:2015-05-28 Published:2015-05-13
Contact: wu guoYING E-mail:wuguoying888@163.com

摘要/Abstract

摘要： 目的研究Nd:YAG激光对鳞状细胞癌（鳞癌）CAL-27细胞的生物学效应。方法用脉冲1064 nm Nd:YAG激光连续照射体外培养处于对数生长期的鳞癌CAL-27细胞,分组照射后倒置显微镜下观察细胞形态，培养1、3、5 d后利用MTT法测定OD值，以此来研究激光对鳞癌CAL-27细胞增殖的影响。结果低剂量激光照射时，CAL-27细胞的OD值随着激光照射剂量的增加而升高（P＜0.05）；而高剂量激光照射时，CAL-27细胞的OD值随着激光照射剂量的增加而降低（P＜0.05）。结论 1 064 nm Nd:YAG激光低剂量照射鳞癌CAL-27细胞，细胞表现形态规则、密度增大，增殖活跃；而高剂量照射鳞癌CAL-27细胞后，细胞形态不规则、密度降低，增殖受到抑制，对指导临床应用有一定参考价值。

关键词: Nd:YAG激光, 鳞癌, 增殖

Abstract: Objective To investigate the biological effect of Nd:YAG laser irradiation on the squamous carcinoma CAL-27 cell proliferation.Methods Squamous carcinoma CAL-27 cells were developed in the vitro,and when they were in the logarithmic phase,the cells were irradiated with different doses of Nd:YAG laser and observed under inverted microscopes.Then,the OD value was measured after 1,3,and 5 days.Results A significant stimulative and inhibitive effect was found respectively of the low and high intensity 1 064 nm laser (P＜0.05) on squamous carcinoma CAL-27 cell proliferation.Conclusions By low intensity 1064 nm Nd:YAG laser,the squamous carcinoma CAL-27 cell proliferation can be stimulated,the cells are morphologically ruled and their density increases.In contrast,the proliferation can be inhibited by high intensity laser,the cells are irregularly shaped and their density decreases.This finding will have a good reference value for the clinical application.

Key words: Nd:YAG laser, squamous carcinoma, proliferation

中图分类号:

R780.2

马浩然吴国英李留炀尚进钱棱陶楠楠钱梦柯王培仇嘉颖. Nd:YAG激光对鳞状细胞癌CAL-27细胞增殖的影响[J]. 口腔医学, 2015, 35(5): 362-364.

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