牙颌发育模式及分子机制

doi:10.13591/j.cnki.kqyx.2023.01.001

口腔医学 ›› 2023, Vol. 43 ›› Issue (1): 1-10.doi: 10.13591/j.cnki.kqyx.2023.01.001

• 院士专栏 • 下一篇

牙颌发育模式及分子机制

张然^1,²,沈宗杉^1,³,吴晓珊^1,⁴,王松灵¹()

1 首都医科大学口腔健康北京实验室,北京(100069)
2 北京大学口腔医院病理科,北京(100034)
3 中山大学附属口腔医院牙周病科,广东广州(510095)
4 中南大学湘雅医院口腔颌面外科,湖南长沙(410008)

修回日期:2022-09-10 出版日期:2023-01-28 发布日期:2023-01-11
通讯作者: 王松灵 E-mail:slwang@ccmu.edu.cn
作者简介:王松灵,中国科学院院士,中国医学科学院学部委员,全国政协委员优秀履职奖获奖者,教授、主任医师。本硕博就读于北京大学医学部,1989年获口腔医学博士学位。现任首都医科大学副校长,中华口腔医学会副会长,北京医学会副会长,口腔健康北京实验室主任。Current Medicine主编,Oral Diseases及JOR副主编,《医学教育管理》主编、《今日口腔》主编。发表论文249篇,其中以主要作者发表英文论文167篇。第一完成人获2003及2010年国家科技进步二等奖两项;获威廉盖茨(William J. Gies)奖、吴阶平医药创新奖、中源协和生命医学奖-成就奖、何梁何利科学技术奖;英国皇家外科学院(爱丁堡)Fellowship ad hominem。研究方向为唾液腺与牙再生。发现人细胞膜硝酸盐转运通道,该通道与硝酸盐对维持机体稳态有重要作用,提出稳态医学的概念,研发基于硝酸盐的耐瑞特新药;揭示牙发育新机制,研发牙髓干细胞新药,成功实现生物性牙齿再生。主编《唾液腺非肿瘤疾病》《口腔颌面疾病临床X线表现及其病理学基础》等学术专著和《口腔医学》《口腔分子生物学与口腔实验动物模型》等本科生和研究生规划教材。获国家发明专利授权19项,美国发明专利授权4项。主持国家杰出青年科学基金、国家自然科学基金重点项目、973、863课题、北京学者及北京市科技重大专项等研究基金。
基金资助:
国家自然科学基金重点项目(82030031);国家自然科学基金重大项目(81991504);国家自然科学基金合作项目(92149301);国家自然科学基金面上项目(82270945);中国医学科学院医学与健康科技创新工程项目创新单元(2019-12M-5-031);北京市科学技术委员会项目(Z181100001718208);北京市教委项目(119207020201);北京大数据精准医疗创新项目(PXM2021_014226_000026);北京市政府“北京学者”项目(PXM2020_014226_000005);北京口腔医院创新团队(CXTD202201)

Molecular mechanisms of tooth, maxilla and mandible development

ZHANG Ran^1,²,SHEN Zongshan^1,³,WU Xiaoshan^1,⁴,WANG Songling¹()

Beijing Laboratory of Oral Health, Capital Medical University, Beijing 100069, China

Revised:2022-09-10 Online:2023-01-28 Published:2023-01-11
Contact: WANG Songling E-mail:slwang@ccmu.edu.cn
Supported by:
PXM2021_014226_000020

摘要/Abstract

摘要：

牙颌发育模式及分子机制是理解牙颌结构功能的前提,也是再生牙颌组织器官的基础。牙发育分为牙胚发育期、牙冠形成期和牙根形成期。在这一过程中,关键基因具有时间空间的序列性表达。牙源性上皮和间充质相互作用以及釉结等特殊细胞群对牙冠形态精细化、个性化调控,发育成牙。在生物应力、信号调控机制下,牙顺利萌出并发挥功能。牙和颌骨同处发育之中,相互独立又相互依存,相互调控共同发育成为一个有机的整体。牙和颌骨均起源于第一鳃弓,牙或颌骨的发育异常通常会导致彼此的发育缺陷。本文评述牙和颌骨发育的过程,首次阐述稳态微环境在牙发育中的作用以及重点关注颌骨发育中以梅克尔软骨为代表的典型结构和特殊的信号调控机制,提出牙颌一体化发育模式,动态解析牙和颌骨一体化发育过程,并期待未来将这些新模式和机制运用于组织再生。

关键词: 牙发育, 颌骨发育, 稳态医学, 梅克尔软骨

Abstract:

Understanding the pattern and molecular mechanisms of tooth, maxilla and mandible development is the prerequisite for studying their regeneration. Tooth development can be divided into three stages: bud-bell stage, tooth crown development stage and tooth root development stage. During these processes, key genes show spatial and temporal expression pattern. Tooth development is a complex process involving interactions between dental epithelium and mesenchyme, precise regulations of enamel knots in cusp patterning, as well as successful eruption into the oral cavity under proper biomechanical stress and signaling transductions. The development of tooth, maxilla and mandible, all of which originate from the first branchial arch, is independent and regulates each other to form a whole during development. Any developmental defects of them will ultimately cause defects to the others. In this paper, we briefly reviewed the development of tooth, maxilla and mandible, proposed that the homeostasis of microenvironment is critical for their development. Moreover, we reviewed the role of Meckel’s cartilage, a special structure and signaling mechanism during mandible development. At last, we proposed an integrated development model of tooth, maxilla and mandible. We also hope that the regeneration of fully functional tooth, maxilla and mandible in human can be achieved based on fundamental knowledge we have gained so far.

Key words: tooth development, maxilla and mandible development, homeostatic medicine, Meckel’s cartilage

中图分类号:

张然,沈宗杉,吴晓珊,王松灵. 牙颌发育模式及分子机制[J]. 口腔医学, 2023, 43(1): 1-10.

ZHANG Ran,SHEN Zongshan,WU Xiaoshan,WANG Songling. Molecular mechanisms of tooth, maxilla and mandible development[J]. Stomatology, 2023, 43(1): 1-10.

图/表 3

参考文献 73

[1]	Antonio N. Ten Cate’s oral histology: development, structure, and function[M]. St Louis: Mosby Inc and affiliate of Elsevier Inc, 2017.
[2]	Yu TS, Klein OD. Molecular and cellular mechanisms of tooth development, homeostasis and repair[J]. Development, 2020, 147(2):dev184754. doi: 10.1242/dev.184754
[3]	Li JY, Parada C, Chai Y. Cellular and molecular mechanisms of tooth root development[J]. Development, 2017, 144(3):374-384. doi: 10.1242/dev.137216 pmid: 28143844
[4]	Zhang R, Teng Y, Zhu L, et al. Odontoblast beta-catenin signaling regulates fenestration of mouse Hertwig’s epithelial root sheath[J]. Sci China Life Sci, 2015, 58(9):876-881. doi: 10.1007/s11427-015-4882-8 pmid: 26208822
[5]	Zhang R, Li TJ. Modulation of microRNAs in tooth root and periodontal tissue development[J]. Curr Stem Cell Res Ther, 2018, 13(2):118-124.
[6]	Clevers H, Nusse R. Wnt/β-catenin signaling and disease[J]. Cell, 2012, 149(6):1192-1205. doi: 10.1016/j.cell.2012.05.012 pmid: 22682243
[7]	Arzoo PS, Klar J, Bergendal B,et al. WNT10A mutations account for 1/4 of population-based isolated oligodontia and show phenotypic correlations[J]. Am J Med Genet, 2014, 164(2):353-359. doi: 10.1002/ajmg.a.36243
[8]	Sasaki T, Ito Y, Xu X, et al. LEF1 is a critical epithelial survival factor during tooth morphogenesis[J]. Dev Biol, 2005, 278(1):130-143. pmid: 15649466
[9]	Chen JQ, Lan Y, Baek JA, et al. Wnt/beta-catenin signaling plays an essential role in activation of odontogenic mesenchyme during early tooth development[J]. Dev Biol, 2009, 334(1):174-185. doi: 10.1016/j.ydbio.2009.07.015 pmid: 19631205
[10]	Liu F, Chu EY, Watt B, et al. Wnt/beta-catenin signaling directs multiple stages of tooth morphogenesis[J]. Dev Biol, 2008, 313(1):210-224. doi: 10.1016/j.ydbio.2007.10.016 pmid: 18022614
[11]	Järvinen E, Shimomura-Kuroki J, Balic A, et al. Mesenchymal Wnt/β-catenin signaling limits tooth number[J]. Development, 2018. doi:10.1242/dev.158048. doi: 10.1242/dev.158048
[12]	Xiong Y, Fang Y, Qian Y, et al. Wnt production in dental epithelium is crucial for tooth differentiation[J]. J Dent Res, 2019, 98(5):580-588. doi: 10.1177/0022034519835194 pmid: 30894046
[13]	Nygaard R, Yu J, Kim J, et al. Structural basis of WLS/evi-mediated Wnt transport and secretion[J]. Cell, 2021, 184(1):194-206.e14. doi: 10.1016/j.cell.2020.11.038 pmid: 33357447
[14]	Lohi M, Tucker AS, Sharpe PT. Expression of Axin2 indicates a role for canonical Wnt signaling in development of the crown and root during pre- and postnatal tooth development[J]. Dev Dyn, 2010, 239(1):160-167.
[15]	Bae CH, Lee JY, Kim TH, et al. Excessive Wnt/β-catenin signaling disturbs tooth-root formation[J]. J Periodontal Res, 2013, 48(4):405-410. doi: 10.1111/jre.12018 pmid: 23050778
[16]	Zhang R, Yang G, Wu XM, et al. Disruption of Wnt/β-catenin signaling in odontoblasts and cementoblasts arrests tooth root development in postnatal mouse teeth[J]. Int J Biol Sci, 2013, 9(3):228-236. doi: 10.7150/ijbs.5476 pmid: 23494738
[17]	Du Y, Ling JQ, Wei X, et al. Wnt/β-catenin signaling particip-ates in cementoblast/osteoblast differentiation of dental follicle cells[J]. Connect Tissue Res, 2012, 53(5):390-397. doi: 10.3109/03008207.2012.668980
[18]	Cam Y, Lesot H, Colosetti P, et al. Distribution of transforming growth factor beta1-binding proteins and low-affinity receptors during odontoblast differentiation in the mouse[J]. Arch Oral Biol, 1997, 42(5):385-391. doi: 10.1016/s0003-9969(97)00017-4 pmid: 9233848
[19]	Li JY, Huang XF, Xu X, et al. SMAD4-mediated WNT signaling controls the fate of cranial neural crest cells during tooth morphogenesis[J]. Development, 2011, 138(10):1977-1989. doi: 10.1242/dev.061341 pmid: 21490069
[20]	Zhang R, Lin JT, Liu Y, et al. Transforming growth factor-βsignaling regulates tooth root dentinogenesis by cooperation with Wnt signaling[J]. Front Cell Dev Biol, 2021, 9: 687099. doi: 10.3389/fcell.2021.687099
[21]	Yu M, Wang H, Fan ZZ, et al. BMP4 mutations in tooth agenesis and low bone mass[J]. Arch Oral Biol, 2019, 103:40-46. doi: S0003-9969(19)30386-3 pmid: 31128441
[22]	Jia SH, Zhou J, Gao Y, et al. Roles of Bmp4 during tooth morphogenesis and sequential tooth formation[J]. Development, 2013, 140(2):423-432. doi: 10.1242/dev.081927 pmid: 23250216
[23]	Huang XF, Xu X, Bringas PJr, et al. Smad4-Shh-Nfic signaling cascade-mediated epithelial-mesenchymal interaction is crucial in regulating tooth root development[J]. J Bone Miner Res, 2010, 25(5):1167-1178. doi: 10.1359/jbmr.091103 pmid: 19888897
[24]	Lim JC, Bae SH, Lee G, et al. Activation of β-catenin by TGF-β1 promotes ligament-fibroblastic differentiation and inhibits cementoblastic differentiation of human periodontal ligament cells[J]. Stem Cells, 2020, 38(12):1612-1623. doi: 10.1002/stem.3275
[25]	Thesleff I. Epithelial-mesenchymal signalling regulating tooth morphogenesis[J]. J Cell Sci, 2003, 116(Pt 9):1647-1648. pmid: 12665545
[26]	Khan M, Seppala M, Zoupa M, et al. Hedgehog pathway gene expression during early development of the molar tooth root in the mouse[J]. Gene Expr Patterns, 2007, 7(3):239-243. pmid: 17095302
[27]	Nakatomi M, Morita I, Eto K, et al. Sonic hedgehog signaling is important in tooth root development[J]. J Dent Res, 2006, 85(5):427-431. pmid: 16632755
[28]	Kettunen P, Laurikkala J, Itäranta P, et al. Associations of FGF-3 and FGF-10 with signaling networks regulating tooth morphogenesis[J]. Dev Dyn, 2000, 219(3):322-332. doi: 10.1002/1097-0177(2000)9999:9999<::AID-DVDY1062>3.0.CO;2-J
[29]	Yokohama-Tamaki T, Ohshima H, Fujiwara N, et al. Cessation of Fgf10 signaling, resulting in a defective dental epithelial stem cell compartment, leads to the transition from crown to root formation[J]. Development, 2006, 133(7):1359-1366. pmid: 16510502
[30]	Fujihara C, Yu KN, Masumoto R, et al. Fibroblast growth factor-2 inhibits CD40-mediated periodontal inflammation[J]. J Cell Physiol, 2019, 234(5):7149-7160. doi: 10.1002/jcp.27469 pmid: 30370560
[31]	Chen HL, Kang J, Zhang FP, et al. SIRT4 regulates rat dental papilla cell differentiation by promoting mitochondrial functions[J]. Int J Biochem Cell Biol, 2021, 134:105962. doi: 10.1016/j.biocel.2021.105962
[32]	Hall BE, Zhang L, Sun ZJ, et al. Conditional TNF-α overexpression in the tooth and alveolar bone results in painful pulpitis and osteitis[J]. J Dent Res, 2016, 95(2):188-195. doi: 10.1177/0022034515612022 pmid: 26503912
[33]	Kimura S, Takeshita N, Oyanagi T, et al. HIF-2α inhibits ameloblast differentiation via Hey2 in tooth development[J]. J Dent Res, 2022: 220345221111971.
[34]	杜文, 罗天, 于海洋. 机械应力相关调控蛋白在小鼠磨牙发育中的时空表达[J]. 四川大学学报(医学版), 2021, 52(1):82-86.
[35]	Wu X, Wang S. Biomechanical stress regulates mammalian tooth replacement[J]. Cell Stress, 2020, 4(3):64-65. doi: 10.15698/cst2020.03.215 pmid: 32190821
[36]	Ravindran S, George A. Multifunctional ECM proteins in bone and teeth[J]. Exp Cell Res, 2014, 325(2):148-154. doi: 10.1016/j.yexcr.2014.01.018 pmid: 24486446
[37]	Hu X, Lin C, Shen B, et al. Conserved odontogenic potential in embryonic dental tissues[J]. J Dent Res, 2014, 93(5):490-495. doi: 10.1177/0022034514523988 pmid: 24554539
[38]	Ye YE, Jiang ZW, Pan YQ, et al. Role and mechanism of BMP4 in bone, craniofacial, and tooth development[J]. Arch Oral Biol, 2022, 140:105465. doi: 10.1016/j.archoralbio.2022.105465
[39]	Jia SH, Kwon HJE, Lan Y, et al. Bmp4-Msx1 signaling and Osr2 control tooth organogenesis through antagonistic regulation of secreted Wnt antagonists[J]. Dev Biol, 2016, 420(1):110-119. doi: S0012-1606(16)30372-4 pmid: 27713059
[40]	Li JJ, Chatzeli L, Panousopoulou E, et al. Epithelial stratification and placode invagination are separable functions in early morphogenesis of the molar tooth[J]. Development, 2016, 143(4):670-681. doi: 10.1242/dev.130187 pmid: 26755699
[41]	Prochazka J, Prochazkova M, Du W, et al. Migration of founder epithelial cells drives proper molar tooth positioning and morphogenesis[J]. Dev Cell, 2015, 35(6):713-724. doi: 10.1016/j.devcel.2015.11.025 pmid: 26702830
[42]	Häärä O, Harjunmaa E, Lindfors PH, et al. Ectodysplasin regulates activator-inhibitor balance in murine tooth development through Fgf20 signaling[J]. Development, 2012, 139(17):3189-3199. doi: 10.1242/dev.079558 pmid: 22833125
[43]	Gao Y, Yang G, Weng T, et al. Disruption of Smad4 in odontoblasts causes multiple keratocystic odontogenic tumors and tooth malformation in mice[J]. Mol Cell Biol, 2009, 29(21):5941-5951. doi: 10.1128/MCB.00706-09 pmid: 19703995
[44]	Feng J, Yang G, Yuan G, et al. Abnormalities in the enamel in bmp2-deficient mice[J]. Cells Tissues Organs, 2011, 194(2/3/4):216-221. doi: 10.1159/000324644
[45]	Li JY, Feng JF, Liu Y, et al. BMP-SHH signaling network controls epithelial stem cell fate via regulation of its niche in the developing tooth[J]. Dev Cell, 2015, 33(2):125-135. doi: 10.1016/j.devcel.2015.02.021
[46]	Bae CH, Kim TH, Ko SO, et al. Wntless regulates dentin apposition and root elongation in the mandibular molar[J]. J Dent Res, 2015, 94(3):439-445. doi: 10.1177/0022034514567198 pmid: 25595365
[47]	Laurikkala J, Kassai Y, Pakkasjärvi L, et al. Identification of a secreted BMP antagonist, ectodin, integrating BMP, FGF, and SHH signals from the tooth enamel knot[J]. Dev Biol, 2003, 264(1):91-105. doi: 10.1016/j.ydbio.2003.08.011 pmid: 14623234
[48]	Wu XS, Hu JR, Li GQ, et al. Biomechanical stress regulates mammalian tooth replacement via the integrin β1-RUNX2-Wnt pathway[J]. EMBOJ, 2020, 39(3):e102374.
[49]	Wang XP. Tooth eruption without roots[J]. J Dent Res, 2013, 92(3):212-214. doi: 10.1177/0022034512474469 pmid: 23345536
[50]	Svandova E, Anthwal N, Tucker AS, et al. Diverse fate of an enigmatic structure: 200 years of meckel’s cartilage[J]. Front Cell Dev Biol, 2020, 8: 821. doi: 10.3389/fcell.2020.00821 pmid: 32984323
[51]	Yuan Y, Chai Y. Regulatory mechanisms of jaw bone and tooth development[J]. Curr Top Dev Biol, 2019, 133: 91-118. doi: S0070-2153(18)30113-3 pmid: 30902260
[52]	Depew MJ, Lufkin T, Rubenstein JLR. Specification of jaw subdivisions by Dlx genes[J]. Science, 2002, 298(5592):381-385. doi: 10.1126/science.1075703 pmid: 12193642
[53]	Wyganowska-Świᶏtkowska M, Przystańska A. The Meckel’s cartilage in human embryonic and early fetal periods[J]. Anat Sci Int, 2011, 86(2):98-107. doi: 10.1007/s12565-010-0093-3 pmid: 20799009
[54]	Mori-Akiyama Y, Akiyama H, Rowitch DH, et al. Sox9 is required for determination of the chondrogenic cell lineage in the cranial neural crest[J]. Proc Natl Acad Sci USA, 2003, 100(16):9360-9365. doi: 10.1073/pnas.1631288100 pmid: 12878728
[55]	Melnick M, Witcher D, Bringas PJr, et al. Meckel’s cartilage differentiation is dependent on hedgehog signaling[J]. Cells Tissues Organs, 2005, 179(4):146-157. doi: 10.1159/000085950
[56]	Yang RT, Zhang C, Liu Y, et al. Autophagy prior to chondrocyte cell death during the degeneration of Meckel’s cartilage[J]. Anat Rec (Hoboken), 2012, 295(5):734-741. doi: 10.1002/ar.22433
[57]	Wang Y, Zheng YQ, Chen D, et al. Enhanced BMP signaling prevents degeneration and leads to endochondral ossification of Meckel’s cartilage in mice[J]. Dev Biol, 2013, 381(2):301-311. doi: 10.1016/j.ydbio.2013.07.016
[58]	Yahiro K, Higashihori N, Moriyama K. Histone methyltransferase Setdb1 is indispensable for Meckel’s cartilage development[J]. Biochem Biophys Res Commun, 2017, 482(4):883-888. doi: 10.1016/j.bbrc.2016.11.128
[59]	Farahat M, Kazi GAS, Hara ES, et al. Effect of biomechanical environment on degeneration of meckel’s cartilage[J]. J Dent Res, 2021, 100(2):171-178. doi: 10.1177/0022034520960118
[60]	Shimada M, Yamamoto M, Wakayama T, et al. Different expression of 25-kDa heat-shock protein (Hsp25) in Meckel’s cartilage compared with other cartilages in the mouse[J]. Anat Embryol, 2003, 206(3):163-173. doi: 10.1007/s00429-002-0297-y pmid: 12592567
[61]	Sakakura Y, Tsuruga E, Irie K, et al. Immunolocalization of receptor activator of nuclear factor-kappaB ligand (RANKL) and osteoprotegerin (OPG) in Meckel’s cartilage compared with developing endochondral bones in mice[J]. J Anat, 2005, 207(4):325-337. doi: 10.1111/j.1469-7580.2005.00466.x
[62]	Zhang ZC, Wlodarczyk BJ, Niederreither K, et al. Fuz regulates craniofacial development through tissue specific responses to signaling factors[J]. PLoS One, 2011, 6(9):e24608. doi: 10.1371/journal.pone.0024608
[63]	Biosse Duplan M, Komla-Ebri D, Heuzé Y, et al. Meckel’s and condylar cartilages anomalies in achondroplasia result in defective development and growth of the mandible[J]. Hum Mol Genet, 2016, 25(14):2997-3010.
[64]	Mansour S, Offiah AC, McDowall S, et al. The phenotype of survivors of campomelic dysplasia[J]. J Med Genet, 2002, 39(8):597-602. pmid: 12161603
[65]	Ricks JE, Ryder VM, Bridgewater LC, et al. Altered mandibular development precedes the time of palate closure in mice homozygous for disproportionate micromelia: An oral clefting model supporting the Pierre-Robin sequence[J]. Teratology, 2002, 65(3):116-120. pmid: 11877774
[66]	Sato T, Kurihara Y, Asai R, et al. An endothelin-1 switch specifies maxillomandibular identity[J]. Proc Natl Acad Sci USA, 2008, 105(48):18806-18811. doi: 10.1073/pnas.0807345105 pmid: 19017795
[67]	Abe M, Cox TC, Firulli AB, et al. GATA3 is essential for separating patterning domains during facial morphogenesis[J]. Development, 2021, 148(17):dev199534. doi: 10.1242/dev.199534
[68]	Marchant C, Anderson P, Schwarz Q, et al. Vessel-derived angiocrine IGF1 promotes Meckel’s cartilage proliferation to drive jaw growth during embryogenesis[J]. Development, 2020, 147(11):dev190488.
[69]	Sugito H, Shibukawa Y, Kinumatsu T, et al. Ihh signaling regulates mandibular symphysis development and growth[J]. J Dent Res, 2011, 90(5):625-631. doi: 10.1177/0022034510397836 pmid: 21297010
[70]	Oka K, Oka S, Sasaki T, et al. The role of TGF-β signaling in regulating chondrogenesis and osteogenesis during mandibular development[J]. Dev Biol, 2007, 303(1):391-404. doi: 10.1016/j.ydbio.2006.11.025
[71]	Shibukawa Y, Young B, Wu CS, et al. Temporomandibular joint formation and condyle growth require Indian hedgehog signaling[J]. Dev Dyn, 2007, 236(2):426-434. doi: 10.1002/dvdy.21036
[72]	Alfaqeeh SA, Gaete M, Tucker AS. Interactions of the tooth and bone during development[J]. J Dent Res, 2013, 92(12):1129-1135. doi: 10.1177/0022034513510321 pmid: 24155263
[73]	Stig H, Anders H. Alveolar ridge resorption after tooth extraction: A consequence of a fundamental principle of bone physiology[J]. J Dent Biomech, 2012(3):1758736012456543.

牙颌发育模式及分子机制

Molecular mechanisms of tooth, maxilla and mandible development

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[3]	吴可明,施洁珺,谷志远. 非综合征型先天性牙发育不全的临床研究[J]. , 2012, 32(2): 103-106.
[4]	吴可明，施洁珺，谷志远. 非综合征型先天性牙发育不全的临床研究[J]. , 2012, 32(02): 103-106.
[5]	徐海艇,王健,徐晓斐,余力,张波,朱昌. 三维CT评价腭裂骨缺损对羊上颌骨发育的影响[J]. , 2011, 31(7): 385-388.