NAF与EGCG联合应用对变异链球菌的作用研究

›› 2021, Vol. 41 ›› Issue (8): 673-677.

• 基础研究 • 下一篇

NAF与EGCG联合应用对变异链球菌的作用研究

刘晓璇,方圆,王瑞

吉林大学口腔医院

收稿日期:2021-01-21 修回日期:2021-02-26 出版日期:2021-08-28 发布日期:2021-08-28
通讯作者: 王瑞 E-mail:w-rui@jlu.edu.cn
基金资助:
吉林省12岁儿童口腔健康状况及相关因素的调查;吉林省儿童窝沟封闭项目的实施现况和效果评价;吉林省儿童窝沟封闭项目的实施现况和效果评价

Effects of Combined Application of NAF and EGCG on Streptococcus mutans

Received:2021-01-21 Revised:2021-02-26 Online:2021-08-28 Published:2021-08-28

摘要/Abstract

摘要： 摘要：目的：研究氟化钠（Sodium fluoride，NAF）与表没食子儿茶素没食子酸酯（Epigallocatechingallate，EGCG）联合应用对变异链球菌的影响。方法：采用微量稀释法分别测量NAF与EGCG对变异链球菌的最小细菌抑制浓度MIC值，采用棋盘微量稀释法测量NAF与EGCG联合作用于变异链球菌的MIC值，并计算FIC指数。结果：NAF单独作用于变异链球菌MIC值为100ppm, EGCG单独作用于变异链球菌的MIC值为500ppm，NAF与EGCG联合应用时MIC值分别为25ppm和62.5ppm,FIC指数为 0.375，FIC指数小于0.5。结论：NAF与EGCG联合应用具有协同抑制变异链球菌的作用。

关键词: 关键词： NAF, EGCG, 变异链球菌, 联合药敏实验

Abstract: [Abstract ] PURPOSE：we study the effects of the combined application of NAF and EGCG on the growth and reproduction of Streptococcus mutans there by inhibiting the occurrence and development of dental caries. Methods: The minimum inhibitory concentration (MIC) of EGCG or NAF against Streptococcus mutans was determined by microdilution method. The potential synergistic/antagonistic effects of the NaF/EGCG combination on the growth of Streptococcus mutans was determined by checkerboard microdilution assays as described previously . Cell growth was examined by OD600 nm. The fractional inhibitory concentration (FIC) was defined as the ratio of the minimum inhibitory concentration (MIC) of an agent used in combination to the MIC of the agent used alone. Results : EGCG inhibited growth of Streptococcus mutans planktonic cells at an MIC of 100ppm and NAF inhibited growth of Streptococcus mutans planktonic cells at an MIC of 500ppm. The fraction of inhibitory concentration (ΣFIC) of the NAF/EGCG combination against Streptococcus mutans was 0.375, indicating a synergistic effect of these 2 agents against Streptococcus mutans planktonic culture. Conclusions: It is shown that the combined effect of NAF and EGCG has a synergistic inhibitory effect on Streptococcus mutans planktonic cells. EGCG can cooperate with NAF to enhance the antibacterial effect of sodium fluoride, thus reducing the toxic effect of NAF , and preventing bacteria from producing fluorine-resistant properties.

Key words: Keywords: NAF, EGCG, Streptococcus mutans , Combined Antimicrobic Susceptibility Test

中图分类号:

R780.2

刘晓璇方圆王瑞. NAF与EGCG联合应用对变异链球菌的作用研究[J]. 口腔医学, 2021, 41(8): 673-677.

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NAF与EGCG联合应用对变异链球菌的作用研究

Effects of Combined Application of NAF and EGCG on Streptococcus mutans

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