转录因子Brn3a及胰岛因子1在三个时期中三叉神经节的表达情况

›› 2016, Vol. 36 ›› Issue (4): 294-298.

转录因子Brn3a及胰岛因子1在三个时期中三叉神经节的表达情况

王瑶¹,姬晓炜¹,贺瑞²,张琼²,张远³,王进涛²,张鹏涛²,张蕾²,史芳川²,钟良军²

1. 新疆医科大学第一附属医院
2. 杭州师范大学附属医院
3. 杭州师范大学附属医院口腔科

收稿日期:2015-11-16 修回日期:2016-01-11 出版日期:2016-04-28 发布日期:2016-04-24
通讯作者: 钟良军 E-mail:zymdyy@163.com
基金资助:
国家自然科学基金

Expression of transcription factor Brn3a and Isl-1 in trigeminal ganglia during three different periods

Received:2015-11-16 Revised:2016-01-11 Online:2016-04-28 Published:2016-04-24

摘要/Abstract

摘要： 目的探讨转录因子Brn3a及胰岛因子1（Islet1,Isl-1）转录因子在生长发育的三个时期中三叉神经节中的表达情况。方法取发育不同时期的小鼠胚胎头部第13.5天、15.5天、17.5天，各个时期为相互对照。用免疫荧光的方法标记相关蛋白，观察比较不同时期的Brn3a及Isl-1在三叉神经节的表达情况。结果三个时期中Brn3a及Isl-1在三叉神经节均有共表达，在生长发育早期Isl-1没有明显增殖，在生长发育晚期Brn3a没有明显凋亡。结论在发育后期Brn3a及Isl-1标记的神经元，依旧共表达于神经元中，且随生长发育，标记Brn3a及Isl-1的神经元逐渐减少。Brn3a及Isl-1标记的神经元，在生长发育过程中分化为亚型神经元，对上下游转录因子起调控作用。

关键词: Brn3a, Isl-1, 三叉神经节

Abstract: Objective To investigate the expression of transcription factor Brn3a and Isl-1 in trigeminal ganglion during the three periods of growth and development. Methods Mouse brains at 13.5, 15.5 and 17.5 days after being born were used for contrast. The immunofluorescence technique was applied to investigate the expression patterns of Brn3 and Isl-1 in trigeminal ganglion between different periods. Results In all three periods, Brn3a and Isl-1 were co-expressed in the trigeminal ganglia. In the early stage of growth and development, Isl-1 showed no significant proliferation, and in the late stage of growth and development, Brn3a showed no significant apoptosis. Conclusion In the late stage of development, Brn3a and Isl-1 marked neurons were still expressed in the neurons, and with the growth and development, the marked Brn3a and Isl-1 neurons gradually decreased. Besides, during the process of growth and development, Brn3a and Isl-1 marked neurons differentiated into subtypes of neurons, which regulated the upstream and downstream transcription factors.

Key words: Brn3a, Isl-1, trigeminal ganglion

中图分类号:

R393

王瑶姬晓炜贺瑞张琼张远王进涛张鹏涛张蕾史芳川钟良军. 转录因子Brn3a及胰岛因子1在三个时期中三叉神经节的表达情况[J]. , 2016, 36(4): 294-298.

参考文献

[1]Badea TC,Williams J,Smallwood P,et al.Combinatorial exprwssion of Brn3 transcription factors in somatosensory neurons:genetic and morphologic analysis[J].J Neurosci,2012,32(3):995-1007
[2]Zou M,Li S,Klein WH,et al.Brn3a/Pou4f4f1 regulates dorsal root ganglion sensory neuron specification and anonal projection into the spinal cord[J].Dev Biol,2012,364(2):144-127
[3]Sajgo S,Ali S,Popescu O,et,al.Dynamic expressiom of transcription factor Brn3b during mouse cranial nerve development[J].[J].J Comp Neuro,2015,29:23890-
[4]Ichikawa H.Qiu FXiang M,et al.Brn-3a is required for the generation of proprioceptors in the mesencephalic trigeminal tract nucleus[J]..Brain Res,2005,1053(1-2):203-206
[5]Lanier J,Dykes IM,Nissen S,et al.Brn3a regulates the transition from neurogenesis titerminal differentiation and repress non-neural gene expression in the teigeminal ganglion[J].Dev Dyn,2009,238(12):3065-3079
[6]Eng SR,Dykes IM,Lanier J,et al.POU-domain factor Brn3a regulates both distinct and common programs of gene expression in the spinal and trigeminal sensory ganglia[J].Neural Dev,2007,2:3-
[7]Ichikawa H,Schulz S,Hollt V,et al.Effect of Brn-3a deficiency on primary nociceptors in the trigeminal ganglion[J].Nrurosci Res,2005,51(4):445-451
[8]Kanafi MM,Mamidi MK,Sureshbabu SK,et al.Generation of islet-like cell aggregates from human non-pancreatic cancer cell lines[J].Biotechol Lett,2014,37(1):227-233
[9]Deng M,Yang H,Xie X,et al.Comparative expression analysis of POU4F1,POU4F2 and ISL1 in developing mouse cochlevestibular ganglion neurons[J].Gene Expr Patterns,2014,15(1):31-37
[10]Dykes IM,Tempest L,Lee SI,et al.Brn3a and islet1 act epistatically to regulate the gene expression program of sensory differentitation[J].J Neurosci,2011,31(27):9789-9799
[11]Sun Y,Dykes IM,Liang X,et al.A centeal role for islet1 in sensory neuron development linking sensory and spinal gene regulatory programs[J].Nat Neurosci,2008,11(11):1288-1293
[12]Schulze-Spate U.Battaglion RFu J,et al.Brn3 transcription factors control terminal osteoclastogensis[J]..J Cell Biochem,2007,102(1):1-12
[13]Huang L,Hu F,Xie X,et al.Pouf41 and Pou4f2 are dispensable for the long-Term survival of adult retinal ganglion cells in mice[J].PLoS ONE,2014,9(4):e94173-
[14]Kaku Y,Ohmori T,Kudo K,et al.Islet1 deletion causes kidney agenesis and hydroureter resembling cakut[J].J Am Soc Nephrol,2013,24(8):1242-1249
[15]Senzaki K,Ozaki S,Yoshikawa M,et al.Runx3 is required for the specification of TrkC-expressing mechanoreceptive trigeminal ganglion neuros[J].Mol Cell Neurosci,2010,43(3):296-307
[16]Dykes IM,Lanier J,Eng SR,et al.Brn3a regulates neuronal subtype specification in the trigeminal ganglion by promoting Runx expression during sensory differentitation[J].Neural Dev,2010,5:3-