EGCG阻断FZD3和MAPK10基因表达调控颌骨牙源性角化囊性瘤转归

›› 2017, Vol. 37 ›› Issue (5): 403-407.

EGCG阻断FZD3和MAPK10基因表达调控颌骨牙源性角化囊性瘤转归

杨银辉¹,王耀钟¹,袁荣涛²,贾暮云²,林锡江¹

1. 青岛市口腔医院
2. 青岛大学附属医院

收稿日期:2016-10-21 修回日期:2017-02-05 出版日期:2017-05-28 发布日期:2017-05-19
通讯作者: 袁荣涛 E-mail:yuanrongtao@163.com
基金资助:
青岛市公共领域科技支撑计划项目

The role of blocking of FZD3 and MAPK10 with EGCG in the treatment of jaw keratocystic odontogenic tumor

Received:2016-10-21 Revised:2017-02-05 Online:2017-05-28 Published:2017-05-19

摘要/Abstract

摘要： 目的研究Wnt信号通路阻断剂表没食子儿茶素没食子酸酯（epigallocatechin-3-gallate，EGCG）在颌骨牙源性角化囊性瘤开窗灌洗治疗中的调控作用。方法收集颌骨牙源性角化囊性瘤标本25例，其中开窗灌洗治疗前病例20例，开窗灌洗治疗术后病例5例，3例生理盐水灌洗，2例EGCG灌洗。应用实时荧光定量PCR及免疫组化方法，检测Wnt信号通路相关基因FZD3及MAPK10的表达，以正常口腔黏膜作为对照。结果 FZD3和MAPK10基因在颌骨牙源性角化囊性瘤中的表达高于口腔正常黏膜，应用生理盐水开窗灌洗治疗后，FZD3和MAPK10的表达降低，但仍高于正常口腔黏膜，而应用EGCG开窗灌洗治疗后，FZD3和MAPK10的表达与正常黏膜差异不大。结论 FZD3和MAPK10在颌骨牙源性角化囊性瘤中高表达，应用EGCG开窗灌洗治疗可使FZD3和MAPK10的表达降低并趋于正常。

关键词: 牙源性角化囊性瘤, 表没食子儿茶素没食子酸酯, Wnt信号通路

Abstract: Objective To study the role of epigallocatechin-3-gallate(EGCG), the inhibitor of Wnt signaling pathway, in the marsupialization treatment of jaw keratocystic odontogenic tumor(KCOT). Methods Twenty-five specimens of KCOT were obtained, twenty before marsupialization, five after marsupialization, three treated with EGCG solution and two with normal saline(NS). The expressions of FZD3 and MAPK10, Wnt signaling pathway genes, were detected. The normal oral mucosa of the same patient was as control. Result Compared to normal oral mucosa, the expressions of FZD3 and MAPK10 in KCOT were up-regulated, which decreased after the treatment of marsupialization with normal saline, but were still higher than those of normal oral mucosa. However, the expressions of FZD3 and MAPK10 in KCOT sharply decreased after the treatment with EGCG and were similar to normal oral mucosa. Conclusion FZD3 and MAPK10 genes are over-expressed in KCOT. Marsupialization with EGCG can block the expressions of FZD3 and MAPK10, which play a role in the treatment of jaw KCOT.

Key words: Epigallocatechin-3-gallate, Keratocystic odontogenic tumors, Wnt signaling pathway

中图分类号:

R739.82

杨银辉王耀钟袁荣涛贾暮云林锡江. EGCG阻断FZD3和MAPK10基因表达调控颌骨牙源性角化囊性瘤转归[J]. , 2017, 37(5): 403-407.

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