关键词: 钛, 微纳米, 拓扑结构, 锌, 成骨细胞, 抗菌性能

Abstract: Objective To prepare zinccontaining micronano patterned titanium surface and preliminarily study its effects on osteoblast behavior and antibacterial properties. Methods Hydrofluoric acidetching, alkali and heattreatment, and hydrothermal treatment with a ZnSO4 solution were performed in sequence to synthesize two zinccontaining micronano topological structures on pure titanium surfaces (15 and 60 minute treatment groups). The SLA titanium surface was used as the control group, and two zinccontaining micronano patterned titanium surfaces were used as the experiment groups. The microtopography and surface elemental compositions of different titanium surfaces were observed and analyzed. MC3T3E1 cells were seeded in the samples of three groups to evaluate the effects of different titanium surfaces on osteoblast behavior. Staphylococcus aureus were seeded in the samples of three groups to investigate antibacterial properties of different titanium surfaces. Results Two micronano topological structures, where uniform networks and contained trace zinc appeared, were prepared on pure titanium surfaces. Compared with the control SLA titanium surface, the titanium surfaces modified by two zinccontaining micronano topological structures significantly upregulated the spreading, proliferation and expressions of osteogenic related proteins of MC3T3E1 cells and had prominent antibacterial properties, especially the 15minute treatment group. Conclusion The zinccontaining micronano topological titanium surface can promote the adhesion, proliferation and differentiation of osteoblasts and has satisfactory antibacterial property.

Key words: titanium, micronano, topological structure, zinc, osteoblast, antibacterial property